Author(s): Karlina Amir Tahir, Khairuddin Djawad, Sartini Sartini, Arief Budiyanto, Ahmad Lalo, Syatirah Jalaluddin, Maulita Indrisari, Syamsuri Syakri, Asrul Ismail, Khaerani, Anshari Masri, Upik A. Miskad

Email(s): upik.miskad@gmail.com

DOI: 10.52711/0974-360X.2022.00688   

Address: Karlina Amir Tahir1, Khairuddin Djawad2, Sartini Sartini3, Arief Budiyanto4, Ahmad Lalo5, Syatirah Jalaluddin6, Maulita Indrisari7, Syamsuri Syakri1, Asrul Ismail1, Khaerani1, Anshari Masri8, Upik A. Miskad9
1Department of Pharmacy, Faculty of Medicine and Health Science, UIN Alauddin Makassar, Makassar, Indonesia.
2Department of Dermatology and Gender, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia.
3Department of Pharmacy and Science, Faculty of Pharmacy, Universitas Hasanuddin, Makassar, Indonesia.
4Department of Dermatology and Gender, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
5National Agency of Drug and Food Control, Makassar, Indonesia.
6Faculty of Medicial and Health Science, UIN Alauddin Makassar, Makassar, Indonesia.
7STIFA, Makassar, Indonesia.
8Department of Pharmacy, Faculty of Medicine and Health Science, Universitas Muhammadiyah Makassar, Indonesia.
9Department of Anatomical Pathology, Faculty of Medicine, Un

Published In:   Volume - 15,      Issue - 9,     Year - 2022


ABSTRACT:
Introduction: Ultraviolet B (UVB) radiation can cause damage to the skin's dermis layer, which can lead to collagen fragmentation. Damage to collagen generates wrinkles, which are a sign of aging. Objective: The purpose of this study was to determine how Siam Weeds cream (Chromolaena odorata L.) altered the thickness and density of collagen in the skin of BALB/c mice after UVB exposure. Methods: There were four groups of 24 BALB/c mice altogether: normal control, negative control (Cream Base with UVB), positive control (UVB), and treatment (C. odorata cream and UVB). Just before and after the mice were exposed to UVB at 500 mJ/cm2, the Siam Weeds cream formulation was applied at a concentration of 1%. Mice were slaughtered, and a back skin excisional biopsy was conducted to analyze collagen. Results: the treatment group differed significantly both in thickness and density of collagen against the positive control group and the negative control group (p<0.05) and There was no statistically significant difference between the treatment and normal control groups (p<0.05). Conclusion: A 1% dosage of C. odorata cream can improve the thickness and density of collagen in UVB-exposed mice's skin.


Cite this article:
Karlina Amir Tahir, Khairuddin Djawad, Sartini Sartini, Arief Budiyanto, Ahmad Lalo, Syatirah Jalaluddin, Maulita Indrisari, Syamsuri Syakri, Asrul Ismail, Khaerani, Anshari Masri, Upik A. Miskad. Collagen Thickness and Density in BALB/c Mice Exposed to UVB Light after using Siam Weeds Cream (Chromolaena odorata L.). Research Journal of Pharmacy and Technology. 2022; 15(9):4099-4. doi: 10.52711/0974-360X.2022.00688

Cite(Electronic):
Karlina Amir Tahir, Khairuddin Djawad, Sartini Sartini, Arief Budiyanto, Ahmad Lalo, Syatirah Jalaluddin, Maulita Indrisari, Syamsuri Syakri, Asrul Ismail, Khaerani, Anshari Masri, Upik A. Miskad. Collagen Thickness and Density in BALB/c Mice Exposed to UVB Light after using Siam Weeds Cream (Chromolaena odorata L.). Research Journal of Pharmacy and Technology. 2022; 15(9):4099-4. doi: 10.52711/0974-360X.2022.00688   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-9-48


REFERENCES:
1.    Kanitakis, J. Anatomy, Histology and Immunohistochemistry of Normal Human Skin. European Journal of Dermatology. 2002. 12 (4) : 390-401
2.    Rachmasari, A., Kartini, R. A., Alviani, G., Solihah, I. Healing Effect of Spray Gel Collagen Extract from Channa striata Bone on Burn in Rats. Research Journal of Pharmaceutical Dosage Form and Technology. 11(4): 275 – 279.
3.    Kolarisck, P. A. Anatomy and Physiology of the Skin. Journal of the Dermatology Nurses’ Association. 2011. 3 : (4) pp 203 - 2011.
4.    Wahyono, P. Impact of UV-B Rays on Photoaging. International Journal of Innovatios in Engineering Research and Technology. 2020. Vol : 7 Issue : 6 pp : 143 - 147.
5.    Jariashvili, K. et al. UV Damage of Collagen: Insights from Model Collagen Peptides. Biopolymers. 2012. 97(3): 189–198.
6.    Frank, R., Gruiji, D. Photocarcinogenesis:UV vs UB Radiation. Skin Pharmacol Appl Skin Physiol. 2002. 15 : 16 - 20.
7.    Parameswari, G., Suriyavathana, M. 2012. In-Vitro Antioxidant Activity of Chromolaena odorata (L.) King & Robinson. International Research Journal of Pharmacy.
8.    Siswanto, Susila, Suyanto. 2015. Metodologi Penelitian Kesehatan dan Kedokteran. Bursa Ilmu Press. ISBN: 978-602-99035-8-4.Yogyakarta.
9.    Vayalil, P.K., Elmets, C.A., Katiyar, S.K. “Treatment of green tea polyphenols in hydrophilic cream prevents UVB-induced oxidation of lipids and proteins, depletion of antioxidant enzymes and phosphorylation of MAPK proteins in SKH-1 hairless mouse skin”, Journal of Carcinogenesis. 2003. 24 (5), 927-936
10.    Fitri, E. W., Anwar, A. I., Djawad, K., Seweng, A., Changara, H., & Alam, G. (2016). The Effectiveness of Topical Mangosteen Pericarp Extract on the Collagen of Mice Skin Exposed to Ultraviolet B. American Journal of Clinical and Experimental Medicine, 4(3): 88-93.
11.    James, W. D. Andrews_ diseases of the skin: Clinical dermatology (10th ed.). 2006. Philadelphia: Elsevier Saunders.
12.    Afaq, F., Mukhtar, H. Botanical Antioxidants in the Prevention of Photocarcinogenesis and Photoaging. Exp Dermatol. 2006. 15 : 678 - 684.
13.    Pittayapruek, P., Meephansan, J., Prapapan, O., Komine, M., Ohtsuki, M. Role of Matrix Metalloproteinases in Photoaging and Photocarcinogenesis: Review Article. International Journal of Molecular Sciences. 2016. pp. 1- 20.
14.    Fisher, G., Wang, Z., Datta, S., Varani, J., Kang, S. Pathophysiology of Premature Skin Aging. N Eng J Med. 2001. 337: 1419-1429.
15.    Choi, S.H. et al. Anti-photoaging effect of jeju putgyul (unripe citrus) extracts on human dermal fibroblasts and ultraviolet B induced hairless mouse skin. Int J Mol Sci. 2017. 18 : E2052.
16.    Choi, Sun-Il. et al. Anti photoaging effect of fermented agricultural by products on ultraviolet B irradiated hairless mouse skin. International Journal of Molecular medicine. 2019. 44: 559-568.
17.    Sindhi, V., Gupta, V., Sharma, K., B. S., K. R., & D. N. Potential Applications of Antioxidants – a review. J Pharm Res. 2013. 7 : 828–35.
18.    Dunaway, S., Odin, R., Zhou, L., Ji, L., Zhang, Y., Kadekaro, A. Natural Antioxidants : Multiple Mechanisms to Protect Skin from Solar Radiation. Front Pharmacol. 2018. 9 : 14.
19.    Trilokchandran, B., Vijayakumar, G., Thippareddy, K. S. Formulation and Evaluation of Cosmetic Cream from Cabbage Extract. Research J Pharm and Tech. 2019. 12(8) : 3589 – 3594.
20.    Roy, A., Sahu, R. K. Formulation and Development of Herbal Sunscreen Cream. Research J Topical and Cosmetic Sci. 5(1): 12 – 14.
21.    Tahir, K.A., Miskad, U.A., Djawad, K., Sartini, S., Djide, N., Khaerani, K., Indrisari, M. Evaluation of Antioxidant Activity of Botto-Botto Leaf Fraction (Chromolaena odorata L.) Using DPPH and ABTS Methods. Open Access Macedonian Journal of Medical Sciences. 2021. 9(A):183-188.

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