Author(s): Ravi Kumar Vakkalagadda, Srinivas Lankalapalli

Email(s): vakkalagaddaravikumar@gmail.com

DOI: 10.52711/0974-360X.2022.00670   

Address: Ravi Kumar Vakkalagadda1*, Srinivas Lankalapalli2
1School of Pharmacy, Guru Nanak Institutions Technical Campus (Autonomous), Hyderabad, Telangana - 501506, India.
2GITAM Institute of Pharmacy, GITAM Deemed to be University, Rushikonda, Visakhapatnam, Andhra Pradesh - 530045, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 9,     Year - 2022


ABSTRACT:
Objective: The objective behind this study was to expose the prospective of ethanolic extract of Ochna obtusata (EEOO) for its in vitro anti-cancer anti-inflammatory and anti-arthritic activities. Methods: The fresh leaves were used to prepare the extracts using petroleum ether, chloroform, ethanol, methanol and water in increasing order of the polarity of solvents. The EEOO was reported to have the presence of a few major phytoconstituents such as, Flavonoids, Phenolics, Glycosides, and Reducing Sugars which was confirmed by preliminary phytochemical screening. The in vitro MTT assay method has been performed with EEOO to evaluate the anti-cancer activity. Similarly, the HRBC Membrane Stabilization assay, Protein denaturation assay, and Protease inhibition assay methods were performed to evaluate the anti-inflammatory and anti-arthritic activity of EEOO. Results and Discussion: From the study, it was confirmed that the various phytoconstituents such as Flavonoids, Phenolics, and Terpenoids were responsible for the anti-cancer activity. Similarly, the prevention of RBC Membrane from its lysis, decrease in albumin denaturation, and protease inhibition was observed which significantly confirmed the anti-inflammatory and anti-arthritic activity of EEOO. Conclusion: It was finally concluded that the phytoconstituents present in the extract were responsible for its anti-cancer, anti-inflammatory, and anti-arthritic activities.


Cite this article:
Ravi Kumar Vakkalagadda, Srinivas Lankalapalli. In vitro Anti-cancer, Anti-inflammatory and Anti-arthritic activity of Ethanolic extract of Ochna obtusata leaves. Research Journal of Pharmacy and Technology. 2022; 15(9):3999-4. doi: 10.52711/0974-360X.2022.00670

Cite(Electronic):
Ravi Kumar Vakkalagadda, Srinivas Lankalapalli. In vitro Anti-cancer, Anti-inflammatory and Anti-arthritic activity of Ethanolic extract of Ochna obtusata leaves. Research Journal of Pharmacy and Technology. 2022; 15(9):3999-4. doi: 10.52711/0974-360X.2022.00670   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-9-30


REFERENCES:
1.    Leber Felix M, Efferth T. Molecular principles of cancer invasion and metastasis (Review). Int J Oncol. 2009;34(4):881-895. doi:10.3892/ijo_00000214
2.    Fry WA, Phillips JL, Menck HR. Ten-year survey of lung cancer treatment and survival in hospitals in the United States. Cancer. 1999;86(9):1867-1876. doi:https://doi.org/10.1002/(SICI)1097-0142(19991101)86:9<1867::AID-CNCR31>3.0.CO;2-9
3.    Stewart BW, Kleihues P. New_Drug.pdf. 2003:181-188.
4.    Adami H-O, Trichopoulos D. Obesity and Mortality from Cancer. N Engl J Med. 2003;348(17):1623-1624. doi:10.1056/nejmp030029
5.    Faridi R, Zahra A, Khan K, Idrees M. Oncogenic potential of Human Papillomavirus (HPV) and its relation with cervical cancer. Virol J. 2011;8(1):269. doi:10.1186/1743-422X-8-269
6.    Gowri SS. In vitro anti-cancer activity of Majidea zanquebarica J. Krik. ex Oliv. (Sapindaceae). Res J Sci Technol. 2020;12(3):173. doi:10.5958/2349-2988.2020.00023.6
7.    Tanavade SS, Naikwade N, Chougule DD. In vitro anti-cancer activity of ethanolic and aqueous extracts of Peristrophe bivalvis Merrill. Res J Pharm Technol. 2012;5(10):1324-1327.
8.    Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol. 2005;100(1):72-79. doi:https://doi.org/10.1016/j.jep.2005.05.011
9.    Bandyopadhyay U, Das D, Banerjee RK. Reactive oxygen species: Oxidative damage and pathogenesis. Curr Sci. 1999;77(5):658-666. http://www.jstor.org/stable/24102839.
10.    Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-126. doi:10.4103/0973-7847.70902
11.    Freeman BA, Crapo JD. Biology of disease: free radicals and tissue injury. Lab Invest. 1982;47(5):412-426.
12.    Jain S, Sherlekar B, Barik R. Evaluation of Antioxidant Potential of Tinospora Cordifolia and Tinospora Sinensis. Ijpsr. 2010;1(11):122-128.
13.    Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 2002;7(9):405-410. doi:10.1016/s1360-1385(02)02312-9
14.    Dua D, Srivastava NS. A study on antioxidant and anti-aging properties of few medicinal plants. Int J Pharm Pharm Sci. 2016;8(1):344-347.
15.    Sheela Rani T, Vadivelu A, Devi L, Teja R, Chitra K, Uma Maheswara Reddy C. In vitro anti arthritic activity of Grewia tiliaefolia (Aerial parts). Res J Pharm Technol. 2011;4(12):1833-1834.
16.    Kamble AA, Khan ND, Khan ZH, Mular SM, Sohail S. In vitro anti-arthritic arthritic activity of vitex negundo and punica granatum. 2017;6(2):5-7.
17.    Saleem TM, Azeem A, Dilip C, Sankar C, Prasanth N, Duraisami R. Anti–inflammatory activity of the leaf extacts of Gendarussa vulgaris Nees. Asian Pac J Trop Biomed. 2011;1(2):147-149. doi:10.1016/S2221-1691(11)60014-2
18.    Oyedapo OA, Adewunmi CO, Iwalewa EO, Makanju VO. Analgesic, antioxidant and anti-inflammatory related activities of 21-hydroxy-2, 41-dimethoxychalcone and 4-hydroxychalcone in mice. J Biol Sci. 2008;8(1):131-136.
19.    Sripradha, Lakshmi T. In vitro Anti- Arthritic activity of Sesbania grandiflora Ethyl acetate extract. Res J Pharm Technol. 2015;8(11):1509-1511. doi:10.5958/0974-360X.2015.00269.3
20.    Hossain MS, Alam MB, Chowdhury NS, et al. Antioxidant, analgesic and anti-inflammatory activities of the herb Eclipta prostrata. J Pharmacol Toxicol. 2011;6(5):468-480.
21.    Hadjigogos K. The role of free radicals in the pathogenesis of rheumatoid arthritis. Panminerva Med. 2003;45(1):7-13.
22.    Alam B, Akter F, Parvin N, et al. Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves. Avicenna J phytomedicine. 2013;3(2):112-125. https://pubmed.ncbi.nlm.nih.gov/25050265.
23.    Liu T, Zhang L, Joo D, Sun S-C. NF-κB signaling in inflammation. Signal Transduct Target Ther. 2017;2:17023. doi:10.1038/sigtrans.2017.23
24.    Guicciardi ME, Gores GJ. Apoptosis: a mechanism of acute and chronic liver injury. Gut. 2005;54(7):1024 LP - 1033. doi:10.1136/gut.2004.053850
25.    Fischer SM, Hawk E, Lubet RA. Non-steroidal Anti-inflammatory Drugs and Coxibs In Chemoprevention: A Commentary Based Primarily on Animal Studies. Cancer Prev Res. July 2011:canprevres.0166.2011. doi:10.1158/1940-6207.CAPR-11-0166
26.    Pal DC, Jain SK. Tribal Medicine. Calcutta: Naya Prokash; 1998.
27.    Indukuri R, Venkata G, Srikanth G, B LP, Raju P. Anti-Diabetic Activity of Ochna obtusata Leaves In Alloxan Induced Rats. 2014;2(1).
28.    B.Venkateswarlu;, Y.Rama R. Evaluation of Diuretic Activity of Ethanol Extract of Ochna obtusata Leaves in Rats. J Pharm Biol. 2013;3(1):14-17. www.jpbjournal.com.
29.    Karimulla S, Kumar BP. Evaluation of Anti-Ulcer Activity of Ochna obtusata in Various Experimental Models. 2012;3(1):14-19.
30.    Gourigari TR, Lepakshi BMD, Kamsala R V., Venkata Raju RR. Evaluation of anticholinergic, antidiabetic and antioxidant activity of leaf extracts of Ochna obtusata DC using in vitro assays. Int J Pharm Pharm Sci. 2016;8(6):82-87.
31.    Vakkalagadda RK, Lankalapalli S. Evaluation of hepatoprotective activity of Ochna obtusata extract on paracetamol induced hepatotoxicity in rats. Int J Pharm Res. 2020;12(4):4886-4894. doi:10.31838/ijpr/2020.12.04.659
32.    Gangoni A, Suneetha B, Sunanda S, Ravindrababu S. Hypolipidemic and Antioxidant Activity of Methanolic Leaf Extract of Ochna obtusata on High Fat Diet Induced Obesity in Rats. Res J Pharmacol Pharmacodyn. 2015;7(1):1. doi:10.5958/2321-5836.2015.00001.4
33.    Penumala M, Zinka RB, Shaik JB, Amooru Gangaiah D. In Vitro Screening of Three Indian Medicinal Plants for Their Phytochemicals, Anticholinesterase, Antiglucosidase, Antioxidant, and Neuroprotective Effects. Chevalot I, ed. Biomed Res Int. 2017;2017:5140506. doi:10.1155/2017/5140506
34.    Plumb JA. Cell Sensitivity Assays: The MTT Assay BT  - Cancer Cell Culture: Methods and Protocols. In: Langdon SP, ed. Totowa, NJ: Humana Press; 2004:165-169. doi:10.1385/1-59259-406-9:165
35.    Ali S, Khan MR, Khan A, Khan R. In vitro anti-cancer activity of extracted oil from Parrotiopsis jacquemontiana (Decne) Rehder. Phytomedicine. 2021;91:153697. doi:10.1016/j.phymed.2021.153697
36.    Lavanya M, Bhaumik A, Reddy AG, Manasa C, Kalyani B, Sushmitha S. Evaluation of Anti-cancer Activity of Ethanolic and Ethylacetoacetate Extracts of Sweet Cherry Against Human Breast Cancer Cell Line MCF-7. Res J Pharmacol Pharmacodyn. 2016;8(2):65. doi:10.5958/2321-5836.2016.00012.4
37.    Kasthuri K, Radha R, Jayshree N, Austin A, Thirugnanasambantham P. Standardization and In Vitro Anti Inflammatory Studies of A Poly Herbal Oil Formulation – Megni. Res J Pharm Technol. 2010;3(3):792-794.
38.    Sureka A, Sharmila CM, Devi RC, Kumar NJM, Banumathi V.  Evaluation of In Vitro Anti Inflammatory activity of Kusta Gaja Kesari-A Siddha Herbo Mineral Formulation against Albumin Protein Denaturation . Asian J Pharm Res. 2018;8(3):145. doi:10.5958/2231-5691.2018.00025.4
39.    Sangeetha G, Vidhya R. In vitro anti-inflammatory activity of different parts of Pedalium murex (L.). Int J Herb Med. 2016;4(3):31-36.
40.    Talbot IC. Pathologic basis of disease. S. L. Robbins, R. S. Cotran and V. Kumar. 3rd edition. W. B. Saunders, Philadelphia, London, Toronto, Mexico City, Rio de Janeiro, Sydney, Tokyo, 1984. No. of pages: x+1467. Price: £49.50. ISBN: 0 7216 7597 2. J Pathol. 1985;147(4):305. doi:https://doi.org/10.1002/path.1711470413
41.    Kumar S, Pandey AK. Chemistry and Biological Activities of Flavonoids: An Overview. Lu KP, Sastre J, eds. Sci World J. 2013;2013:162750. doi:10.1155/2013/162750
42.    Boominathan M, Bakiyalakshmi S V.  In Vitro Anti Arthritic activity of Njavara Rice . Asian J Pharm Res. 2016;6(4):250. doi:10.5958/2231-5691.2016.00035.6
43.    Maleki SJ, Crespo JF, Cabanillas B. Anti-inflammatory effects of flavonoids. Food Chem. 2019;299:125124. doi:https://doi.org/10.1016/j.foodchem.2019.125124

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