Author(s): Dwi Murtiastutik, Afif Nurul Hidayati, Septiana Widyantari, Astindari, Bernadya Yogatri A. Saputri, Lunardi Bintanjoyo, Evy Ervianti, Damayanti, Maylita Sari

Email(s): dwimurtiastutik@yahoo.co.id

DOI: 10.52711/0974-360X.2022.00630   

Address: Dwi Murtiastutik1*, Afif Nurul Hidayati1,2, Septiana Widyantari1, Astindari1, Bernadya Yogatri A. Saputri1, Lunardi Bintanjoyo1, Evy Ervianti1, Damayanti1, Maylita Sari1
1Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya 60285, Indonesia.
2Universitas Airlangga Teaching Hospital, Surabaya 60115, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 8,     Year - 2022


ABSTRACT:
Background: Oral candidiasis is an infection due to the activity of Candida albicans in the oral cavity. Oral candidiasis is one of the most common opportunistic infections occuring among Human Immunodeficiency Virus (HIV)/Acquired immune deficiency syndrome (AIDS) patients. Due to increasing resistance and adverse effects to commonly used antifun¬gal drugs, many recent studies have examined the use of herbal essential oils as antifungal agents. In this study, essential oil of Rosmarinus officinalis (Lamiaceae) and nystatin were examined for in vitro antifungal activ¬ity against Candida species. Aim: To evaluate antifungal activity of essential oil of Rosmarinus officinalis (Lamiaceae) and nystatin by comparing inhibition zone diameters. Methods: This study was an experimental laboratory study with a posttest only design conducted in Dr. Soetomo General Academic Hospital, Surabaya. Forty isolates consisted of 20 isolates of Candida albicans and Candida non-albicans were subjected to test for antifungal activity using the diffusion disk method using paper discs or blank discs and inhibitory zones were recorded. Results: Diffusion test results revealed stronger antifungal effect of nystatin against all analyzed Candida strains. This study showed the mean diameter of the inhibitory zone for Candida albicans formed by rosemary essential oil is 2.25 mm and the average inhibition zone formed by rosemary essential oil for Candida non-albicans is 1.5 mm. Conclusion: The antifungal activity of nystatin is stronger when compared to rosemary essential oil as seen from a greater inhibition zone than rosemary essential oil in the diffusion method.


Cite this article:
Dwi Murtiastutik, Afif Nurul Hidayati, Septiana Widyantari, Astindari, Bernadya Yogatri A. Saputri, Lunardi Bintanjoyo, Evy Ervianti, Damayanti, Maylita Sari. Evaluation of the Antifungal effects of Rosemary Oil and comparison with Nystatin on the Growth of Candida species isolates from HIV/AIDS patients with Oral Candidiasis. Research Journal of Pharmacy and Technology. 2022; 15(8):3755-8. doi: 10.52711/0974-360X.2022.00630

Cite(Electronic):
Dwi Murtiastutik, Afif Nurul Hidayati, Septiana Widyantari, Astindari, Bernadya Yogatri A. Saputri, Lunardi Bintanjoyo, Evy Ervianti, Damayanti, Maylita Sari. Evaluation of the Antifungal effects of Rosemary Oil and comparison with Nystatin on the Growth of Candida species isolates from HIV/AIDS patients with Oral Candidiasis. Research Journal of Pharmacy and Technology. 2022; 15(8):3755-8. doi: 10.52711/0974-360X.2022.00630   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-8-72


REFERENCES:
1.     Abdulaziz SM, Shaswary IA, Muhammad AA. In vitro antifungal activity of essential oils from local plants against fluconazole-resistant oral Candida albicans isolates. Zanco J Med Sci. 2015; 19(2):965–71.
2.     da Silva ICG, de Pontes Santos HB, Cavalcanti YW, Nonaka CFW, de Sousa SA, de Castro RD. Antifungal Activity of Eugenol and its Association with Nystatin on Candida albicans. Pesqui Bras Odontopediatria Clin Integr. 2017; 17(1):e3235.
3.     Sakkas H, Gousia P, Economou V, Petsios S, Papadopoulou C. Antifungal activity of four essential oils against Candida clinical isolates. Asian J Ethnopharmacol Med Foods. 2016; 2(1):22–5.
4.     Jahani S, Bazi S, Shahi Z, Asadi MS, Mosavi F, Baigi GS. Antifungal Effect of the Extract of the Plants Against Candida albicans. Int J Infect. 2017; 4(2):e36807.
5.     Carvalhinho S, Costa AM, Coelho AC, Martins E, Sampaio A. Susceptibilities of Candida albicans Mouth Isolates to Antifungal Agents, Essentials Oils and Mouth Rinses. Mycopathologia. 2012; 174:69–76.
6.     Cavalcanti YW, de Almeida L de FD, Padilha WWN. Anti-adherent activity of Rosmarinus officinalis essential oil on Candida albicans : an SEM analysis. Rev Odonto Cienc. 2011; 26(2):139–44.
7.     Patturaja K, Geetha R V. Evaluation of Antimycotic Activity of Three Essential Oils on Candida Albicans -An Invitro Study. J Pharm Sci Res. 2017; 9(4):480–2.
8.     Nejati H, Farahpour MR, Nagadehi MN. Topical Rosemary officinalis essential oil improves wound healing against disseminated Candida albicans infection in rat model. Comp Clin Path. 2015; 24:1377–83.
9.     Moghtader M, Salari H, Farahmand A. Evaluation of the antifungal effects of rosemary oil and comparison with synthetic borneol and fungicide on the growth of Aspergillus flavus. J Ecol Nat Environ. 2011; 3(6):210–4.
10.     Andayani N, Susilowati A, Pangastuti A. Anti candida minyak atsiri lengkuas putih (Alpinia galanga) terhadap Candida albicans penyebab candidiasis secara invitro. EL-Vivo. 2014; 2(2):1–9.
11.     Kengne M, Shu SV, Nwobegahay JM, Achonduh O. Antifungals susceptibility pattern of Candida spp. isolated from female genital tract at the Yaoundé Bethesda Hospital in Cameroon. Pan Afr Med J. 2017; 28:294.
12.     Murtiastutik D, Maharani CS, Rahmadewi, Listiawan MY. Nystatin Profile on Candida Species in HIV / AIDS Patients with Oral Candidiasis : A Phenomenology Study. J Pure Appl Microbiol. 2019; 13(4):2013–9.
13.     Lely N, Pratiwi RI, Imanda YL. Efektivitas Antijamur Kombinasi Ketokonazol dengan Minyak Atsiri Sereh Wangi (Cymbopogon nardus ( L .) Rendle). Indones J Appl Sci. 2017; 7(2):10–5.
14.     Nenoff P, Krüger C, Neumeister C, Schwantes U, Koch D. In vitro susceptibility testing of yeasts to nystatin – low minimum inhibitory concentrations suggest no indication of in vitro resistance of Candida albicans, Candida species or non-Candida yeast species to nystatin. Clin Med Investig. 2016; 1(3).
15.     Khorram Z, Hakimaneh SM, Naeini A, Rafieinezhad R, Salari AM, Shayegh SS. The Antifungal Effects of Two Herbal Essences in Comparison with Nystatin on the Candida Strains Isolated from the Edentulous Patients. J Contemp Dent Pract. 2019; 20(6):716–9.
16.     Ćosić J, Vrandečić K, Postić J, Jurković D, Ravlić M. In vitro antifungal activity of essential oils on growth of phytopathogenic fungi. Poljoprivreda. 2010; 16(2):25–8.
17.     Bozin B, Mimica-Dukic N, Samojlik I, Jovin E. Antimicrobial and Antioxidant Properties of Rosemary and Sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) Essential Oils. J Agric Food Chem. 2007; 55:7879–85.
18.     Murtiastutik D, Listiawan MY, Bintanjoyo L, Hidayati AN, Widyantari S, Astindari, et al. Ketoconazole: A Re-emerging choice for Oral candidiasis in patients with human immunodeficiency virus infection/acquired Immunodeficiency Syndrome. Res J Pharm Technol. 2022;15(3):1071–6.
19.     Rezeki S, Pradono SA, Subita GP, Rosana Y, Sunnati S, Gani BA. The antifungal susceptibility of Candida albicans isolated from HIV/AIDS patients. Dent J (Majalah Kedokt Gigi). 2021;54(2):82–6.
20.     Reza NR, Tantari SHW, Basuki S. Uji Kepekaan In Vitro Flukonazol Terhadap Spesies Candida penyebab Kandidiasis Oral Pada Pasien HIV/AIDS dengan Vitek II (In Vitro Susceptibilty Test of Fluconazole to Candida spp in Patients with Oropharyngeal Candidiasis and HIV/AIDS with Vitek II). Berk Ilmu Kesehat Kulit dan Kelamin - Period Dermatology Venereol. 2017;29(3):234–42.

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