Ali M. Abbed, Muna A. Shakir, Ali abdulrasool Hussein, Shaema H. Abdulsada, Lubna F. Mohammed, Ahmed M. Lifatah
email@example.com , firstname.lastname@example.org , AliMa84@uomustansiriyah.edu.iq
Ali M. Abbed1, Muna A. Shakir1, Ali abdulrasool Hussein2, Shaema H. Abdulsada1, Lubna F. Mohammed1, Ahmed M. Lifatah1
1Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq.
2College of medicine, Mustansiriyah University, Baghdad, Iraq.
Volume - 15,
Issue - 8,
Year - 2022
Many researches have focused on the health and therapeutics properties and behaviour of phytochemical products, that including bee products. Bee pollen comprises on very large compounds such as flavonoids, phenolic acids and terpenoids, which have inhibiting effect on some enzymes. Monoamine oxidase (MAO) was an important metabolic enzyme. The aims of this study was to evaluation the effects of aqueous and ethanolic extracts of bee pollen on MAO and activities in human sera. The identification of extracts constituents was carried out by GC-Ms analysis technique. The results of the present study manifested the effectiveness of these two extracts which have an inhibitory effects on MAO. Different extracts and volumes have been used in this study which shows the inhibition percentage at maximum level equal to (79, 57) % in MAO and (57.33) % in ethanolic and aqueous extracts respectively. The recorded Km values in ethanolic extract were higher than Km in the aqueous extract, whereas the recorded Vmax values in aqueous extract were higher than Vmax in ethanolic extract. On the other hand, GC-Ms analysis of bee pollen extracts revealed the existence of 40 compounds.
Cite this article:
Ali M. Abbed, Muna A. Shakir, Ali abdulrasool Hussein, Shaema H. Abdulsada, Lubna F. Mohammed, Ahmed M. Lifatah. Effect of Aqueous and Alcoholic Bee Pollen Extracts on Monoamine Oxidase Activity. Research Journal of Pharmacy and Technology. 2022; 15(8):3731-5. doi: 10.52711/0974-360X.2022.00625
Ali M. Abbed, Muna A. Shakir, Ali abdulrasool Hussein, Shaema H. Abdulsada, Lubna F. Mohammed, Ahmed M. Lifatah. Effect of Aqueous and Alcoholic Bee Pollen Extracts on Monoamine Oxidase Activity. Research Journal of Pharmacy and Technology. 2022; 15(8):3731-5. doi: 10.52711/0974-360X.2022.00625 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-8-67
1. Anna Rzepecka-Stojko, Jerzy Stojko, Krzysztof Jasik and Ewa Buszman. Anti-Atherogenic Activity of Polyphenol-Rich Extract from Bee Pollen. Nutrients J. 2017, pp.1-17.
2. Almaraz-Abarca N., Campos M.D.G., Ávila-Reyes J. A., Naranjo-Jiménez N., Herrera Corral J., González-Valdez L.S. Variability of antioxidant activity among honeybee collected pollen of different botanical origin. Interciencia J. 2004, p:574–578.
3. Karsten Munsttedt, Benjamin Voss, UWE Kullmer, Ursula Schneider R,and Jutta Hubner. Bee pollen and honey for the alleviation of hot flushes and other menopausal symptoms in breast cancer patients. Mol Clin Oncol. 2015, p:869–874.
4. Stefan Bogdanov, Pollen: Production, Nutrition and Health: A Review, Bee Product Science, 2014, p. 3.
5. Katarzyna Komosinska-Vassev, Pawel Olczyk, Justyna Kafmierczak,1 Lukasz Mencner and Krystyna Olczyk, "Bee Pollen: Chemical Composition and Therapeutic Application", Hindawi Publishing Corporation, 2015, p.1-6.
6. Estevinho LM, Rodrigues S, Pereira AP, Feás X. Portuguese bee pollen: palynological study, nutritional and microbiological evaluation. International Journal of Food Science and Technology, 2012; vol .47. p. 429–35.
7. Bruna Estefânia Diniz Frias, Cosme Damião Barbosa and Anete Pedro Lourenço. Pollen nutrition in honey bees (Apis mellifera): impact on adult health. Apidologie Journal. 2016, vol. 47, p.15–25.
8. Pascoal A, Rodrigues S, Teixeira A, Feás X, Estevinho LM. Biological activities of commercial bee pollens: Antimicrobial, antimutagenic, antioxidant and antiinflammatory. Food and Chemical Toxicology, 2014; 63:233–9.
9. Guiné RPF. Bee Pollen: Chemical Composition and Potential Beneficial Effects on Health. Current Nutrition and Food Science, 2015, p. 301-308.
10. Oktay YJ., Zehra Can, Özlem Saral, Esin Yulu L, Ferhat Öztürk, Rezzan Aliyaz JcJo Llu, Sinan Canpolat and Sevgi Kolayl J. Hepatoprotective potential of chestnut bee pollen on carbon tetrachloride-induced hepatic damages in rats. Evidence-based Complementary and Alternative Medicine Hindawi Publishing Corporation. 2013, p:9.
11. A. Pascoal, S. Rodrigues, A. Teixeira, X. Feas, and L. M. Estev. Biological activities of commercial bee pollens: antimicrobial, antimutagenic, antioxidant and anti-inflammatory. Food and Chemical Toxicology. 2014, vol. 63, p: 233–239.
12. Edmondson, D, E. Binda, C. Monoamine Oxidase. Subcell Biochem. 2018; 87: 117-139.
13. Najlaa Qasim Muftin. The Effect of Some Organic Compounds on Monoamino oxidase Activity. Journal of Al-Nahrain University. 2012, vol.15 (4), p:38-46.
14. Grady Meghan M., Stahl Stephen M. Practical guide for prescribing MAOIs: debunking myths and removing barriers. CNS Spectrums. 2012, 17, p: 2–10.
15. Stefan Bogdanov. Pollen: Production, Nutrition and Health: A Review . Bee Product Science. 2017, p: 1-36.
16. Prabhuv. Effects of nardostachysjatamansi on biogenic amines and inhibitory amino acids in the rat brain. Planta Med. 1994, 60. P:114-117.
17. Leja M, Mareczek A, Wyżgolik G, Klepacz-Baniak J, Czekońska, K. Antioxidative properties of bee pollen in selected plant species. Food Chem. 2007, 100, 237–240.
18. Claudia B, Paige N, Frantisek H, Dale, E and Andrea M. Structure of human monoamine oxidase B, a drug target for the treatment of neurological disorders. Nature Structural Biology. 2002; 9: 22-26.
19. Graikou K, Kapeta S, Aligiannis N, Sotiroudis G, Chondrogianni N, Gonos E, Chinou I. Chemical analysis of Greek pollen—Antioxidant, antimicrobial, and proteasome activation properties. Chem. Cent. J. 2011, 5, doi:10.1186/1752-153X-5-33
20. Gulcin, I. Antioxidant activity of food constituents: An overview. Arch. Toxicol. 2012, 86, 345–391.
21. Robert, S et al. Characterization of Lavender essential oils by using gas chromatography-mass spectrometry with correlation of linear retention indices and comparison with comprehensive two-dimensional gas chromatography. Journal of Chromatography. 2002; A,1: 000-000.
22. W. Jennings, T. Shibamoto. Qualitative Analysis of Flavor and Fragrance Volatiles by Glass Capillary Gas Chromatography, Academic Press, New York, 1980.
23. Wan A.W.O., Noorfatemah Y., Zainab A.G., Nurdianah H.F. GC-Ms analysis of Chemical constituents in ethanolic Bee pollen extracts from three species of Malaysian stingless Bee. J. APIC. SCI. 2018; 62(2): 275-284.