Author(s): Asma Abid, Messaouda Dekmouche, Lazhar Bechki, Kamilia Bireche, Hakim Belkhalfa, Abdeldjabbar Messaoudi, Mohamed Lakhder Belfar

Email(s): asma.abid94@gmail.com

DOI: 10.52711/0974-360X.2022.00564   

Address: Asma Abid1*, Messaouda Dekmouche1,2, Lazhar Bechki1,2, Kamilia Bireche1, Hakim Belkhalfa3, Abdeldjabbar Messaoudi1, Mohamed Lakhder Belfar1,2
1Laboratoire de Valorisation et Promotion des Ressources Sahariennes (VPRS), Université de Kasdi Merbah, Ouargla, Algérie.
2Département de Chimie, Université de Kasdi Merbah, Ouargla, Algérie.
3Centre de Recherche Scientifique et Technique en Analyse Physico-chimique (CRAPC), Ouargla, Algérie.
*Corresponding Author

Published In:   Volume - 15,      Issue - 8,     Year - 2022


ABSTRACT:
Phytochemical compounds are known to be components of many plants and herbs; they have received great interest from the public and scientists due to their health benefits as antioxidants. The aim of the present study was to reveal the bioactive compounds and evaluation of antioxidant activity of the dichloromethane, ethyl acetate, n-butanol and residual water extracts of Atractylis aristata. The bioactive compounds analysis was investigated using HPLC-UV analysis obtained at 254nm and optimized with 16 standards, ABTS and DPPH methods were used for estimating the antioxidant capacity. Thirteen bioactive compounds were identified in the extracts by comparing the retention time. Major compounds detected in the extracts were Acetylsalicylic acid, Ascorbic acid, Gallic acid, Quercetin and Vanillin. All extracts give an antioxidant capacity varied with polarity of solvents. The residual water extract demonstrated a significant amount of total phenolics, flavonoids and condensed tannins (3.544±0.738mg of GAE/g DW, 3.104±0.6760mg of QE/g DW and 2.692±0.561mg of CE/g DW, respectively). In two methods tested to evaluate the antioxidant activity, ethyl acetate extract displayed the highest antioxidant capacity (IC50 value: 0.097±0.003mg/ml in DPPH assay and IC50 value: 0.077±0.003mg/ml in ABTS assay).


Cite this article:
Asma Abid, Messaouda Dekmouche, Lazhar Bechki, Kamilia Bireche, Hakim Belkhalfa, Abdeldjabbar Messaoudi, Mohamed Lakhder Belfar. Bioactive Composition Analysis using HPLC-UV Profile and Evaluation of Antioxidant activities of different extracts from Aerial parts of Atractylis aristata batt. Research Journal of Pharmacy and Technology. 2022; 15(8):3370-6. doi: 10.52711/0974-360X.2022.00564

Cite(Electronic):
Asma Abid, Messaouda Dekmouche, Lazhar Bechki, Kamilia Bireche, Hakim Belkhalfa, Abdeldjabbar Messaoudi, Mohamed Lakhder Belfar. Bioactive Composition Analysis using HPLC-UV Profile and Evaluation of Antioxidant activities of different extracts from Aerial parts of Atractylis aristata batt. Research Journal of Pharmacy and Technology. 2022; 15(8):3370-6. doi: 10.52711/0974-360X.2022.00564   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-8-6


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