Author(s): Ivon Diah Wittiarika, Agus Sulistyono, Budi Prasetyo, Lunardhi Susanto, Rize Budi Amalia, Erni Rosita Dewi


DOI: 10.52711/0974-360X.2022.00610   

Address: Ivon Diah Wittiarika1*, Agus Sulistyono2, Budi Prasetyo1,2, Lunardhi Susanto3, Rize Budi Amalia1, Erni Rosita Dewi1
1School of Midwifery, Faculty of Medicine, Universitas Airlangga, Surabaya Indonesia.
2Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Airlangga/Soetomo Teaching Hospital, Surabaya, Indonesia.
3School of Pharmacy, University of Hang Tuah, Surabaya Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 8,     Year - 2022

Introduction: Maternal stress is harmful to the placenta. It induces apoptosis. Maternal folic acid (FA) supplementation is mandatory in Indonesia and other countries. FA is known as an antioxidant and probably reduces the apoptosis index in placenta with maternal stress. Aim: To investigate the folic acid supplementation in pregnant stress mice (Mus Musculus) on the apoptosis index and placental TNFa expression. Methods: Twenty-one Mus musculus strainded balb/c female mice were randomly divided into three groups. The first group was normal mice, the second group was given stress, and the third group was given stress and folic acid supplementation (3 mg/kg body weight/day in 0,5ml suspension). The ovulation was induced by pregnant mare's serum gonadotropin and HCG. The stress was given by immobilizing the mice in a transparent pipe and giving bright light twice a day on gestational day 10-15. On gestational day 16, the mice were sacrificed, and the placenta tissue was taken. The expression of TNF-a and apoptotic index was calculated by multiplying the score for % stained cells and staining intensity. Result: The expression of TNF-a (mean±SD) of the normal, second, and third groups were 2.1±0.37, 5.9±1.01, and 3.2±1.78. The apoptotic index (mean±SD) of the normal, second, and third groups were 2.7±1.09, 6.3±0.87, and 3.2±0.65. Conclusion: Folic acid supplementation reduces TNF-a and apoptotic index expression in the placenta with maternal stress, and there is a positive correlation between TNF-a and apoptotic index.

Cite this article:
Ivon Diah Wittiarika, Agus Sulistyono, Budi Prasetyo, Lunardhi Susanto, Rize Budi Amalia, Erni Rosita Dewi. Folic Acid Supplementation in Pregnant Mice: An Approach to reduce the Expression of TNF-A and Placental Apoptosis Index in Maternal Stress. Research Journal of Pharmacy and Technology. 2022; 15(8):3645-0. doi: 10.52711/0974-360X.2022.00610

Ivon Diah Wittiarika, Agus Sulistyono, Budi Prasetyo, Lunardhi Susanto, Rize Budi Amalia, Erni Rosita Dewi. Folic Acid Supplementation in Pregnant Mice: An Approach to reduce the Expression of TNF-A and Placental Apoptosis Index in Maternal Stress. Research Journal of Pharmacy and Technology. 2022; 15(8):3645-0. doi: 10.52711/0974-360X.2022.00610   Available on:

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