Author(s):
M. Mayuri, B. Pushpa Kumari, M. Kishore Babu, M. Hima Saila, Sk. Niha Faiz, D. Ranganayakulu
Email(s):
mayuri.jayanag@gmail.com , pushpahema3@gmail.com
DOI:
10.52711/0974-360X.2022.00562 
Address:
M. Mayuri1*, B. Pushpa Kumari2, M. Kishore Babu3, M. Hima Saila4, Sk. Niha Faiz5, D. Ranganayakulu6
1Department of Pharmacology, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur - 603203, Tamil Nadu, India.
2Department of Pharmacology, Krishna Teja Pharmacy College, Tirupati, Andhra Pradesh, India.
3Department of Pharmaceutics, Krishna Teja Pharmacy College, Tirupati, Andhra Pradesh, India.
4,5,6Department of Pharmacology, Sri Padmavathi School of Pharmacy, Tirupati, Andhra Pradesh, India.
*Corresponding Author
Published In:
Volume - 15,
Issue - 8,
Year - 2022
ABSTRACT:
Osteoporosis is a life-threatening condition that primarily affects women. Mice, rats, dogs, and rabbits have all been used as research animals. Bone strength, bone micro architectural characteristics, and osteoporosis risk are all affected by genetic factors. In this sequence, Type I collagen is the primary bone protein encoded by the COLIA1 and COLIA2 genes. The goal of the study is to develop new model for osteoporosis in rats by using N-Ethyl-N- Nitrosourea (ENU) which particularly targets COL1A1. Further, we treated the osteoporotic rats with Collagen type protein, in order to cross check the disease induction. And Rats induced with ENU, exhibited mutation in COL1A1 gene increased in the levels of both serum and urine biochemical parameters like calcium, phosphorus, ALP and creatinine. PCR analysis reported the mutation in COL1A1 gene. The use of animal models of osteoporosis is required to better understand the complex nature of this condition and to create new preventive and treatment approaches.
Cite this article:
M. Mayuri, B. Pushpa Kumari, M. Kishore Babu, M. Hima Saila, Sk. Niha Faiz, D. Ranganayakulu. N-Ethyl-N-Nitrosourea (ENU) induced Osteoporosis in Rats by inducing mutation in Collagen Type 1 protein–A Novel Method Development. Research Journal of Pharmacy and Technology. 2022; 15(8):3360-4. doi: 10.52711/0974-360X.2022.00562
Cite(Electronic):
M. Mayuri, B. Pushpa Kumari, M. Kishore Babu, M. Hima Saila, Sk. Niha Faiz, D. Ranganayakulu. N-Ethyl-N-Nitrosourea (ENU) induced Osteoporosis in Rats by inducing mutation in Collagen Type 1 protein–A Novel Method Development. Research Journal of Pharmacy and Technology. 2022; 15(8):3360-4. doi: 10.52711/0974-360X.2022.00562 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-8-4
REFERENCES:
1. Taichman RS. Blood and bone: two tissues whose fates are intertwined to create the hematopoietic stem-cell niche. Blood. 2005; 105 (7):2631-2639. DOI: 10.1182/blood-2004-06-2480
2. Grant SF. Ralston SH. Genes and osteoporosis. Trends Endocrinol Metab. 1997; 8 (6):232-236. DOI: 10.1016/s1043-2760(97)00058-1
3. Marini JC. Lewis MB. Wang Q. Chen KJ. Orrison BM. Serine for glycine substitution in type I collagen in two cases of type IV Osteogenesis imperfecta (OI): additional evidence for a regional model of OI Pathophysiology. J Biol Chem. 2010; 268: 2667–2673. PMID: 8094076
4. Muyonga JH. Cole CG. Duodu KG. Fourier transforms infrared (FTIR) spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Latesniloticus). Food Chem. 2004; 86: 325-332. DOI 10.1016/j.foodchem.2003.09.038
5. Christodoulou S. Goula T. Ververidis A. Drosos G. Vitamin D and Bone Disease. Dragana: Hindawi Publishing Corporation; 2013. https://doi.org/10.1155/2013/396541
6. Laird E. Ward M. McSorley E. Strain J. Wallace J. Vitamin D and Bone Health; Potential Mechanisms. Nutrients. 2010;2(7):693-724. doi: 10.3390/nu2070693
7. Brzezińska O. Łukasik Z. Makowska J. Walczak K. Role of Vitamin C in Osteoporosis Development and Treatment-A Literature Review. Nutrients. 2020;12(8):2394. Published 2020 Aug 10. Doi:10.3390/nu120823. doi: 10.3390/nu12082394
8. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983; 16 (2):109-110. DOI: 10.1016/0304-3959(83)90201-4
9. Justice MJ. Carpenter DA. Favor J. Neuhauser-Klaus A. Hrabé de Angelis M. Soewarto D. Moser A. Cordes S. Miller D. Chapman V. Weber JS. Rinchik EM. Hunsicker PR. Russell WL. Bode VC. Effects of ENU dosage on mouse strains. Mamm Genome. 2000; 11 (7):484-488. DOI: 10.1007/978-1-60761-974-1_18
10. Tabeta K. Du X. Arimatsu K. Yokoji M. Takahashi N. Amizuka N. Hasegawa T. Crozat K. Maekawa T. Miyauchi S. Matsuda Y. Ida T. Kaku M. Hoebe K. Ohno K. Yoshie H. Yamazaki K. Moresco EMY.Beutler B. An ENU-induced splice site mutation of mouse Col1a1 causing recessive osteogenesis imperfecta and revealing a novel splicing rescue. Sci Rep. 2017; 7(1):11717. doi: 10.1038/s41598-017-10343-9
11. Helena M. Silva RM. Dumont VC. Neves JS. Mansur HS. Guilherme L and Heneine D. Extraction and characterization of highly purified collagen from bovine pericardium for potential bioengineering applications. Mater SciEng C Mater Biol Appl. 2013;33(2):790-800. DOI: 10.1016/j.msec.2012.11.003
12. Park JK. Lee EM. Kim AY. Lee EJ. Min CW. Kang KK. Lee MM. Jeong KS. Vitamin C deficiency accelerates bone loss inducing an increase in PPAR-gamma expression in SMP30 knockout mice. Int J Exp Pathol. 2012; 93(5):332-340. doi: 10.1111/j.1365-2613.2012.00820.x
13. Peterkofsky B. Ascorbate requirement for hydroxylation and secretion of procollagen: relationship to inhibition of collagen synthesis in scurvy. Am J Clin Nutr. 1991;54(Suppl 6):S1135–S1140. DOI: 10.1093/ajcn/54.6.1135s
14. Sugimoto T. Nakada M. Fukase M. Imai Y. Kinoshita Y. Fujita TL. Effects of ascorbic acid on alkaline phosphatise activity and hormone responsiveness in the osteoblastic osteosarcoma cell line UMR-106. Calcif Tissue Int. 1986; 39:171–174. DOI: 10.1016/j.jinorgbio.2017.03.006
15. Kipp DE. McElvain M. Kimmel DB. Akhter MP. Robinson RG. Lukert BP. Scurvy results in decreased collagen synthesis and bone density in the guinea pig animal model. Bone. 1996;18:281–288. DOI: 10.1016/8756-3282(95)00481-5
16. Baylink DJ. Finkelman RD. Mohan S. Growth factors to stimulate bone formation. J Bone Miner Res. 1993; 8 Suppl 2:S565–572. DOI: 10.1002/jbmr.5650081326
17. Temu TM. Wu KY. Gruppuso PA. Phornphutkul C. The mechanism of ascorbic acid-induced differentiation of ATDC5 chondrogenic cells. Am J Physiol Endocrinol Metab. 2010; 299 (2):E325–334. doi: 10.1152/ajpendo.00145.2010
18. Hooshmand S. Brisco JR. Arjmandi BH. The effect of dried plum on serum levels of receptor activator of NF-kappaB ligand, osteoprotegerin and sclerostin in osteopenic postmenopausal women: a randomised controlled trial. Br J Nutr. 2014; 112:55–60. doi: 10.1017/S0007114514000671
19. Takeuchi Y. Nakayama K. Matsumoto T. Differentiation and cell surface expression of transforming growth factor-beta receptors are regulated by interaction with matrix collagen in murine osteoblastic cells. J Biol Chem 1996;271(7):3938–3944. DOI: 10.1074/jbc.271.7.3938