Author(s): Noer Ulfah, Samuel Rehuel Santoso, Lambang Bargowo, Shafira Kurnia, Chiquita Prahasanti

Email(s): chiquita-p-s@fkg.unair.ac.id

DOI: 10.52711/0974-360X.2022.00586   

Address: Noer Ulfah1, Samuel Rehuel Santoso2, Lambang Bargowo1, Shafira Kurnia1, Chiquita Prahasanti3
1Department of Periodontology, Faculty of Dental Medicine Universitas Airlangga, Jl. Prof. Dr. Moestopo No. 47 Surabaya – Indonesia.
2Periodontology Specialist Program Student, Faculty of Dental Medicine Universitas Airlangga, Jl. Prof. Dr. Moestopo No. 47 Surabaya – Indonesia.
3Professor of Department of Periodontology, Faculty of Dental Medicine Universitas Airlangga, Jl. Prof. Dr. Moestopo No. 47 Surabaya – Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 8,     Year - 2022


ABSTRACT:
Background: Damage to the periodontium tissue requires a regenerative treatment to increase the dimensions of the lost bone. This research was conducted to find another regenerative material with the use of type 1 collagen peptides derived from gourami fish scales. This study was conducted to test the viability of gourami scales collagen peptides on Human Gingival Fibroblast cells for 24 hours. Purpose: To determine the viability of Human Gingival Fibroblasts (HGF) after the administration of collagen peptides of gourami fish scales. Method: HGF was taken from healthy gingiva and planted in 96 well plates. The type 1 collagen peptides of gourami fish scales with concentrations of 0.32 mg / ml, 0.16 mg / ml, 0.04 mg / ml, 0.02 mg / ml and 0.01 mg / ml were inserted into each well and incubated for 24 hours. MTT Assay was performed to see the viability of fibroblast cells. Results: There was an increase in the viability value from a concentration of 0.32 mg/ml to 0.01 mg/ml. The concentration of 0.01 mg/ml showed the highest viability. Conclusion: The collagen peptide is a potential substance for tissue engineering. The concentration of 0.01 mg/ml collagen peptides shows the highest HGF viability.


Cite this article:
Noer Ulfah, Samuel Rehuel Santoso, Lambang Bargowo, Shafira Kurnia, Chiquita Prahasanti. The Viability of Collagen Peptide from Osphronemus goramy Fish Scale Extract on Human Gingival Fibroblast. Research Journal of Pharmacy and Technology. 2022; 15(8):3497-1. doi: 10.52711/0974-360X.2022.00586

Cite(Electronic):
Noer Ulfah, Samuel Rehuel Santoso, Lambang Bargowo, Shafira Kurnia, Chiquita Prahasanti. The Viability of Collagen Peptide from Osphronemus goramy Fish Scale Extract on Human Gingival Fibroblast. Research Journal of Pharmacy and Technology. 2022; 15(8):3497-1. doi: 10.52711/0974-360X.2022.00586   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-8-28


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