Nedhaal S. Zbar, Zaid N. Abbas, Dhafar N. Al-Ugaili
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Nedhaal S. Zbar, Zaid N. Abbas, Dhafar N. Al-Ugaili*
Department of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq.
Volume - 15,
Issue - 7,
Year - 2022
Tonsillitis which is commonly referred to a strep throat is caused by group A ß-hemolytic streptococci (GABHS) or Streptococcus pyogenes. The bacteria is consider as one of the major human-specific bacterial pathogen and one of its virulence factors is the streptokinase; for this reason, 100 tonsillitis swabs were taken from Iraqi children suffering from symptoms of sore throat. Using conventional cultural methods, 60 bacterial isolates were characterized and subjected to biochemical tests. As a result, 40 isolates were identified as hemolytic bacteria and only 6 of them were identified as Streptococcus. pyogens using Bacitracin (0.04) and Vitek 2 system. The ability of the 6 isolates for streptokinase production was screened and the results showed that all of them were produce the enzyme with variable degrees. Molecular detection of streptokinase gene using real time PCR technique has been done with specific primers and the amplification results were present with all S.pyogens strains.
Cite this article:
Nedhaal S. Zbar, Zaid N. Abbas, Dhafar N. Al-Ugaili. Detection of Streptokinase Production by Streptococcus pyogen using Real Time PCR. Research Journal of Pharmacy and Technology. 2022; 15(7):3251-4. doi: 10.52711/0974-360X.2022.00545
Nedhaal S. Zbar, Zaid N. Abbas, Dhafar N. Al-Ugaili. Detection of Streptokinase Production by Streptococcus pyogen using Real Time PCR. Research Journal of Pharmacy and Technology. 2022; 15(7):3251-4. doi: 10.52711/0974-360X.2022.00545 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-7-67
1. Anderson J. Paterek E. Tonsillitis. Stat Pearls Publishing, Treasure Island (FL): 2020.
2. Nitzsche R. Köhler J. Kreikemeyer B. Oehmcke-Hecht S. Streptococcus pyogenes escapes killing from extracellular histones through plasminogen binding and activation by streptokinase. J. Innate Immun. 2016. 8. 589-600.
3. Monisha V. Preethika G. Devi CS. Screening for Streptokinase producing Streptococcus sp. from Food and Soil samples. Research Journal of Pharmacy and Technology. 2018. 11. 2845-2847.
4. Ahmed YT. Zbar NS. Al-Shaibani AB. Detection of Hyaluronidase and Streptokinase Virulence Factors from Streptococcus pyogenes. Al-Nahrain Journal of Science. 2015. 18. 136-140.
5. Kharaeva ZF. Mustafaev MS. Blieva LZ. Hulaev IV. Gendugova OM. Bozieva DS. Dynamics Persistent Potential of Bacteria as a Treatment Target in Pyoinflammatory Diseases. Research Journal of Pharmacy and Technology. 2018. 11. 5304-5312.
6. Molaee N. Abtahi H. Mosayebi G. Expression of recombinant streptokinase from streptococcus pyogenes and its reaction with infected human and murine sera. Iran J Basic Med Sci. 2013. 16. 985-989.
7. Huish S. Thelwell C. Longstaff C. Activity Regulation by Fibrinogen and Fibrin of Streptokinase from Streptococcus Pyogenes. PLOS ONE. 2017. 12. e0170936. 10.1371/journal.pone.0170936
8. Babashamsi M. Razavian MH. Nejadmoghaddam MR. Production and purification of streptokinase by protected affinity chromatography. Avicenna J Med Biotechnol. 2009. 1. 47-51.
9. Forbes BA. Sahm DF. Weissfeld AS. Bailey WR. Bailey & Scott's diagnostic microbiology. Elsevier Mosby: St. Louis, Mo. 2007.
10. Rijal K. Dhakal N. Shah R. Timilsina S. Mahato P. Thapa S. Ghimire P. Antibiotic susceptibility of Group A Streptococcus isolated from throat swab culture of school children in Pokhara, Nepal. Nepal Med Coll J. 2009. 11. 238-40.
11. Brahmadathan KN. Gladstone P. Microbiological diagnosis of streptococcal pharyngitis: lacunae and their implications. Indian J Med Microbiol. 2006. 24. 92-6. 10.4103/0255-0857.25178
12. Bhardwaj S. Angayarkanni J. Bhattacharya S. Das A. Palaniswamy M. Isolation, screening and characterization of β-haemolytic streptococci with potential of streptokinase production. Int Res J Biol Sci. 2013. 2. 63-66.
13. Rajendran V. Selvan K. Production and partial purification of streptokinase from Streptococcus pyogenes. Journal of Biochemical Technology. 2011. 3. 289-291.
14. Nikitha G. Chowdary K. Vani KN. D'Souza MG. Bhupal G. Swathi B. Kumar VV, et al. Comparative Study on Streptokinase, Reteplase and Tenecteplase in the management of ST-Elevated Myocardial Infarction. Research Journal of Pharmacy and Technology. 2020. 13. 160-162.
15. Spellerberg B. Brandt C. Laboratory diagnosis of Streptococcus pyogenes (group A streptococci). Streptococcus pyogenes: Basic Biology to Clinical Manifestations [Internet]. 2016.
16. Mihaila-Amrouche L. Bouvet A. Loubinoux J. Clonal spread of emm type 28 isolates of Streptococcus pyogenes that are multiresistant to antibiotics. Journal of Clinical Microbiology. 2004. 42. 3844-3846.
17. Espy MJ. Uhl JR. Sloan LM. Buckwalter SP. Jones MF. Vetter EA. Yao JDC, et al. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev. 2006. 19. 165-256. 10.1128/CMR.19.1.165-256.2006