Lidia K. Al-Halaseh, Nariman A. Al-Jawabri, Hayat Al-Btoush, Rawan Al-Suhaimat, Siba Majali, Maha N. Abu Hajleh, Ali Al-Samydai, Mai A.M. Ahmed
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Lidia K. Al-Halaseh1*, Nariman A. Al-Jawabri1, Hayat Al-Btoush1, Rawan Al-Suhaimat1, Siba Majali1, Maha N. Abu Hajleh2, Ali Al-Samydai3, Mai A.M. Ahmed4
1Department of Pharmaceutical Chemistry, Faulty of Pharmacy, Mutah University, Zip code (61710), Al-Karak, Jordan.
2Department of Cosmetic Science, Pharmacological and Diagnostic Research Centre, Faculty of Allied Medical Science, Al-Ahliyya Amman University, Zip code (19328), Amman, Jordan.
3Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Al-Ahliyya Amman University, Zip code (19328), Amman, Jordan.
4Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
Volume - 15,
Issue - 7,
Year - 2022
Objectives: Pennisetum setaceum has been used traditionally as herbal hypoglycemic and slimming medicine by Jordanians. However, its claimed benefits are still elusive. The current in vivo study aimed to justify the folk use of this herbal tea and evaluate its effect. Materials and methods: plant material was collected from South Jordan and identified. Crude aqueous extract of Pennisetum setaceum aerial parts was prepared. The lethal dose was estimated after given groups of mice oral doses of the herbal extract (1, 2, 4, 6, 8 and 10 g/Kg mouse weight). The potential weight reduction activity was measured by recording the animal weight for 30 days after feeding them 1 g/Kg oral doses of the herbal medicine for 10 consecutive days. The hypoglycemic activity was evaluated in two different concentrations: 1 and 2 g/Kg mouse weight. Male mice with an average weight of 26.5 ± 2.76 g were rendered diabetic by an intraperitoneal dose of streptozocin. Groups of mice were given the herbal medication orally. A negative control group was given d. water. Blood sugar levels were measured from tail blood every 30 min for 3 h. LD50 was estimated. Results: The plant was identified to be P. setaceum by the local authority in Jordan. LD50 was estimated to be 10 g/Kg mouse weight after oral administration. Significant weight reduction of the orally fed group (27 ± 3.1 to 24 ± 2.2) with minor undesirable effects. A significant decrease in blood glucose level in the treated diabetic animals were measured compared with the control group. Significant variation in time-dependent hypoglycemic effect was found between the two tested doses. Conclusion: P. setaceum aqueous extract can be considered as a promising candidate for amelioration of blood sugar profile with potential slimming activity.
Cite this article:
Lidia K. Al-Halaseh, Nariman A. Al-Jawabri, Hayat Al-Btoush, Rawan Al-Suhaimat, Siba Majali, Maha N. Abu Hajleh, Ali Al-Samydai, Mai A.M. Ahmed. In vivo investigation of the potential hypoglycemic activity of Pennisetum setaceum: Justification of the traditional use among Jordanians. Research Journal of Pharmacy and Technology. 2022; 15(7):3185-9. doi: 10.52711/0974-360X.2022.00533
Lidia K. Al-Halaseh, Nariman A. Al-Jawabri, Hayat Al-Btoush, Rawan Al-Suhaimat, Siba Majali, Maha N. Abu Hajleh, Ali Al-Samydai, Mai A.M. Ahmed. In vivo investigation of the potential hypoglycemic activity of Pennisetum setaceum: Justification of the traditional use among Jordanians. Research Journal of Pharmacy and Technology. 2022; 15(7):3185-9. doi: 10.52711/0974-360X.2022.00533 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-7-55
1. Abu-Irmaileh BE. Afifi FU. Herbal medicine in Jordan with special emphasis on commonly used herbs. Journal of Ethnopharmacology. 2003;89(2-3):193-7. doi: 10.1016/s0378-8741(03)00283-6.
2. Wazaify M. Alawwa I. Yasein N. Al-Saleh A. Afifi FU. Complementary and alternative medicine (CAM) use among Jordanian patients with chronic diseases. Complementary Therapies in Clinical Practice. 2013;19(3):153-7. doi.org/10.1016/j.ctcp.2013.03.001
3. Abdel-Qader DH. Albassam A. Ismael NS. Al Meslamani AZ. Chen LC. Banat HF. Mansoor K. Hamadi S. Herbal medicines use in the Jordanian population: A nationally representative cross-sectional survey. Journal of Pharmacy & Pharmacognosy Research. 2020;8(2):525-36.
4. Kasabri V. Afifi FU. Hamdan I. In vitro and in vivo acute antihyperglycemic effects of five selected indigenous plants from Jordan used in traditional medicine. Journal of Ethnopharmacology. 2011;133(2):888-96. doi.org/10.1016/j.jep.2010.11.025
5. Shaw JE. Sicree RA. Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Research and Clinical Practice. 2010;87(1):4-14. doi.org/10.1016/j.diabres.2009.10.007
6. Khader Y. Batieha A. Ajlouni H. El-Khateeb M. Ajlouni K. Obesity in Jordan: prevalence, associated factors, comorbidities, and change in prevalence over ten years. Metabolic Syndrome and Related Disorders. 2008;6(2):113-20.doi.org/10.1089/met.2007.0030
7. Liu Y. Sun M. Yao H. Liu Y. Gao R. Herbal medicine for the treatment of obesity: an overview of scientific evidence from 2007 to 2017. Evidence-Based Complementary and Alternative Medicine.2017.doi.org/10.1155/2017/8943059
8. Dabelea D. The predisposition to obesity and diabetes in offspring of diabetic mothers. Diabetes Care. 2007;30(S-2):169-74. doi.org/10.2337/dc07-s211
9. Astrup A. Finer N. Redefining type 2 diabetes:‘diabesity’or ‘obesity dependent diabetes mellitus’?. Obesity Reviews. 2000;1(2):57-9. doi.org/10.1046/j.1467-789x.2000.00013.x
10. Ahmed SS. Prakash KC. Tabassum S. Salma N. Devi KA. Antidiarrhoeal Potential of various Solvent Extracts of Achyranthes aspera against three diverse diarrhoeal animal models. Research Journal of Pharmacy and Technology. 2021;14(8):4173-7.doi:10.52711/0974-360X.2021.00722
11. Ansori AN. Kharisma VD. Fadholly A. Tacharina MR. Antonius Y. Parikesit AA. Severe acute respiratory syndrome coronavirus-2 emergence and its treatment with alternative medicines: A review. Research Journal of Pharmacy and Technology. 2021;14(10):5551-7. doi.org/10.52711/0974-360X.2021.00967
12. Regitha RN. Parthasarathy V. Balakrishnan N. Cancer Protective effect of Brassicca nigra and Role of its Chemical Constituents. Research Journal of Pharmacy and Technology. 2021;14(2):1115-21. doi: 10.5958/0974-360X.2021.00201.8
13. Issa R. Research article use of herbal remedies, conventional medicine, diet and exercise for weight Loss: Case Study of University Students in Jordan. Pakistan Journal of Nutrition. 2018;17:76-88. doi: 10.3923/pjn.2018.76.88
14. Preethi PJ. Herbal medicine for diabetes mellitus: A Review. Asian Journal of Pharmaceutical Research. 2013;3(2):57-70.doi:10.7439/ijpp
15. Kumbhare M. Sivakumar T. Surana A. Evaluation of Hypoglycemic potential of Moringa oleifera bark extracts on normal and Alloxanized diabetic rats. Research Journal of Pharmaceutical Dosage Forms and Technology. 2021;13(2):95-9. doi:10.52711/0975-4377.2021.00017
16. Pradeep S. Anindh S. Tanushree C. Natural products with potent hypoglycemic activity. Research Journal of Pharmacy and Technology. 2010;3(3):650-6. doi: 10.5958/0974-360X
17. El-Desoky GE. Aboul-Soud MA. Al-Numair KS. Antidiabetic and hypolipidemic effects of Ceylon cinnamon (Cinnamomum verum) in alloxan-diabetic rats. Journal of Medicinal Plants Research. 2012;6(9):1685-91. doi.org/10.5897/JMPR11.1472
18. de Las Heras N. Valero-Muñoz M. Martín-Fernández B. Ballesteros S. López-Farré A. Ruiz-Roso B. Lahera V. Molecular factors involved in the hypolipidemic-and insulin-sensitizing effects of a ginger (Zingiber officinale Roscoe) extract in rats fed a high-fat diet. Applied Physiology, Nutrition, and Metabolism. 2017;42(2):209-15. doi.org/10.1139/apnm-2016-0374
19. Wang X. Liu Q. Zhu H. Wang H. Kang J. Shen Z. Chen R. Flavanols from the Camellia sinensis var. assamica and their hypoglycemic and hypolipidemic activities. Acta Pharmaceutica Sinica B. 2017;7(3):342-6. doi.org/10.1016/j.apsb.2016.12.007
20. Aisa H. Xin X. Tang D. Chapter 40 - Nigella sativa: A Medicinal and Edible Plant That Ameliorates Diabetes. Bioactive Food as Dietary Interventions for Diabetes (2nd Ed). 2019; Academic press. 629-40. https://doi.org/10.1016/B978-0-12-813822-9.00040-0
21. Haghighatsiar N. Askari G. Saraf-Bank S. Feizi A. Keshmiri H. Effect of flaxseed powder on cardiovascular risk factor in dyslipidemic and hypertensive patients. International Journal of Preventive Medicine. 2019;10:218. doi: 10.4103/ijpvm.IJPVM_563_17
22. Dominic AA. Parkavi C. Murugaiah K. Dhanaraj TS. Hypolipidemic activity of Cyperous rotundus on CCl4 induced dyslipidemia in rats. Asian Journal of Pharmacy and Technology. 2012;2(2):51-3.
23. Mohammed SM. Abd-Almonuim AE. Majeed A. Khlaf H. The Anti-Diabetic and Anti-Hyperlipidemia effect of Citrus maxima Fruit Peel extract in Streptozotocin-Induced Diabetic Rats. Research Journal of Pharmacy and Technology. 2021;14(10):5355-8. doi: 10.52711/0974-360X.2021.00934
24. Chigozie IJ. Chidinma IC. Hypoglycemic, hypocholesterolemic, anti-anemic and ocular-protective effects of an aqueous extract of the rhizomes of Sansevieria liberica Gérôme and Labroy (Agavaceae) on alloxan induced diabetic Wistar rats. Asian Journal of Research and Pharmceutical Sciences.2011;1(4):137-48.
25. Ali KH. Al-Jawad FH. Kadhim HM. The possible hepatoprotecive effects of Krill oil and silymarin against carbon tetrachloride (CCl4)-Induced rats model of liver fibrosis: in vivo study'. Research Journal of Pharmacy and Technology. 2021;14(11):5953-8.doi: 10.52711/0974-360X.2021.01034
26. Chan T. Potential risks associated with the use of herbal anti-obesity products. Drug-Safety. 2009;32:453-6.doi.org/10.2165/00002018-200932060-00002
27. Saini S. Nanda S. Dhiman A. Elemental Analysis in Piper betle Linn. and Jatropha gossypifolia Linn. leaves: Biosafety Studies. Research Journal of Pharmacy and Technology. 2018;11(11):5078-82.doi: 10.5958/0974-360X.2018.00926.5
28. Oran SA. Plant diversity of Al- Balqa Governorate, Jordan. International Journal of Biodiversity and Conservation. 2016; 8 (5): 93-104. doi.org/10.5897/IJBC2014.0741
29. Taifour H. El-Oqlah A. The plants of Jordan: an annotated checklist. Kew publishing; 2017: Jordan. Royal Botanic Garden ISBN: 978-9957-8709-2-8 National Library Deposit: 2017/8/4356 BOOK
30. Al-Halaseh L. Hajleh MA. Al-Samydai A. Weight reduction in response to unauthorized treatment in a poorly controlled obese woman. Biomedicine. 2022;42(2):410-2. doi.org/10.51248/.v42i2.1291
31. Beckwith AG. Zhang Y. Seeram NP. Cameron AC. Nair MG. Relationship of light quantity and anthocyanin production in Pennisetum setaceum cvs. Rubrum and Red Riding Hood. Journal of Agricultural and Food Chemistry. 2004;52(3):456-61.doi.org/10.1021/jf034821+
32. Maduro Dias CSAM. Vouzela CFM. Rosa HJD. Madruga JS. Borba AES. Nutritional Potential of Some Invasive Species of Macaronesia for Ruminants. American Journal of Agricultural Research. 2020; 5:102.
33. Deeds MC. Anderson JM. Armstrong AS. Gastineau DA. Hiddinga HJ. Jahangir A. Eberhardt NL. Kudva YC. Single dose streptozotocin-induced diabetes: considerations for study design in islet transplantation models. Laboratory Animals. 2011;45(3):131-40. doi: 10.1258/la.2010.010090
34. Williamson EM. Okpako DT. Evans FJ. Pharmacological Methods in Phytotherapy Research. Selection, Preparation and Pharmacological Evaluation of Plant Material. Vol. 1. John Wiley & Sons, New York. 1996;155-68.
35. Matsuo T. Izumori K. D-Psicose inhibits intestinal α-glucosidase and suppresses the glycemic response after ingestion of carbohydrates in rats. Journal of Clinical Biochemistry and Nutrition. 2009;45(2):202-6. doi.org/10.3164/jcbn.09-36
36. Phung OJ. Scholle JM. Talwar M. Coleman CI. Effect of noninsulin antidiabetic drugs added to metformin therapy on glycemic control, weight gain, and hypoglycemia in type 2 diabetes. Jama. 2010;303(14):1410-8. doi:10.1001/jama.2010.405