Author(s):
Elvan Wiyarta, Kusmardi Kusmardi, Yurnadi Hanafi Midoen
Email(s):
yurnadi.kes@ui.ac.id
DOI:
10.52711/0974-360X.2022.00532 
Address:
Elvan Wiyarta1, Kusmardi Kusmardi2,3,4, Yurnadi Hanafi Midoen5*
1Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430 Indonesia.
2Departement of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430 Indonesia.
3Drug Development Research Cluster, Indonesia Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia.
4Human Cancer Research Center, Indonesia Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430 Indonesia.
5Departement of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia.
*Corresponding Author
Published In:
Volume - 15,
Issue - 7,
Year - 2022
ABSTRACT:
Objective: Colorectal cancer (CC) is one type of cancer with a high incidence worldwide. Many therapeutic techniques have been for CC but have not yet yielded satisfactory results. Fish oil has potential as an alternative therapy for CC through its anti-inflammatory effects. Here, we want to investigate that effect on TNF-a expression using omega-3-rich fish oil (FO). Methods: FO was injected into Swiss mice that have been induced (2% in drinking water) with DSS for seven consecutive days. Animals were separated into six groups: normal, negative control, positive control, solvent control, and FO groups (3 and 6 mg/kg body weight/day). All animals were sacrificed, and the colons were collected then stained with anti-TNF-a. The stained sections were subsequently examined with ImageJ based on colour density. Results: Based on the H-Score of each group, FO 3 mg and 6 mg has significantly decreased the expression of TNF-a compared to the negative control (p=0.001 and p=0.009). Conclusion: FO administration was able to inhibit the expression of TNF-a on mice's colonic tissue induced with AOM and DSS.
Cite this article:
Elvan Wiyarta, Kusmardi Kusmardi, Yurnadi Hanafi Midoen. Effect of Omega-3-Rich Fish Oil on TNF- Expression in Mice's Colonic Tissue Induced with Azoxymethane (AOM) and Dextran Sodium Sulphate (DSS). Research Journal of Pharmacy and Technology. 2022; 15(7):3179-4. doi: 10.52711/0974-360X.2022.00532
Cite(Electronic):
Elvan Wiyarta, Kusmardi Kusmardi, Yurnadi Hanafi Midoen. Effect of Omega-3-Rich Fish Oil on TNF- Expression in Mice's Colonic Tissue Induced with Azoxymethane (AOM) and Dextran Sodium Sulphate (DSS). Research Journal of Pharmacy and Technology. 2022; 15(7):3179-4. doi: 10.52711/0974-360X.2022.00532 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-7-54
REFERENCES:
1. Kuipers EJ. Grady WM. Lieberman D. Seufferlein T. Sung JJ. Boelens PG. van de Velde CJH et al Colorectal cancer. Nature Reviews Disease Primers. 2015; 1:1-25. doi:10.1038/nrdp.2015.65
2. GLOBOCAN. Estimated cancer incidence, mortality and prevalence in 2012 [Internet]. International Agency for Research on Cancer–WHO; 2012 [cited 2020 May 20]. Available from: http://globocan.iarc.fr/Pages/fact_sheets_cancer.aspx
3. Widjaja S. Yo H. Colorectal cancer in Indonesia - a centre report. Annals of Oncology. 2016 Jun; 27(Suppl 2): ii97. doi: 10.1093/annonc/mdw201.46
4. Khairina D. Suzanna E. Triana D. Kadir A. Widyastuti TH. Sulistyowati LS. Rosalina I et al Profile of colorectal cancer in 14 provinces in Indonesia. Journal of Global Oncology. 2018 4: Supplement 2, 66s-66s. doi:10.1200/jgo.18.64300
5. Bhutia SK. Maiti TK. Targeting tumors with peptides from natural sources. Trends in Biotechnology. 2008 Apr;26(4): 210–17. doi:10.1016/j.tibtech.2008.01.002
6. Calder PC. Omega-3 fatty acids and inflammatory processes: from molecules to man. Biochemical Society Transactions. 2017 Oct 15; 45(5): 1105-15. doi: https://doi.org/10.1042
7. Volpato M. Hull MA. Omega-3 polyunsaturated fatty acids as adjuvant therapy of colorectal cancer. Cancer and Metastasis Reviews. 2018 Sep;37(2-3): 545-55. doi:10.1007/s10555-018-9744-y.
8. Morland SL. Martins KJB. Mazurak VC. n-3 polyunsaturated fatty acid supplementation during cancer chemotherapy. Journal of Nutrition & Intermediary Metabolism. 2016 Sep; 5 (1): 107-16. doi.org/10.1016/j.jnim.2016.05.001
9. Patterson WL. Georgel PT. Breaking the cycle: the role of omega-3 polyunsaturated fatty acids in inflammation-driven cancers. Biochemistry and Cell Biology. 2014 Oct;92(5):321-8. doi: 10.1139/bcb-2013-0127
10. Vergili-Nelsen JM. Benefits of fish oil supplementation for haemodialysis patients. Journal of the American Dietetic Association. 2003 Sep;103(9):1174-7. doi: 10.1016/s0002-8223(03)00984-2.
11. Lee JY. Sim TB. Lee J. Na HK. Chemopreventive and chemotherapeutic effects of fish oil derived omega-3 polyunsaturated fatty acids on colon carcinogenesis. Clinical Nutrition Research. 2017 Jul; 6(3): 147–160. doi:10.7762/cnr.2017.6.3.147
12. Kusmardi. Priosoeryanto BP. Harlina E. Cornain S. Inhibition activities of fish oil in inos, cox-2, and β-catenin expressions in colorectal preneoplasia of mice induced by azoxymethane and dextran sodium sulphate. Journal of Applied Biotechnology. 2014; 2 (2): 91-9. doi:10.5296/jab.v2i2.6294
13. Zins K. Abraham D. Sioud M. Aharinejad S. Colon cancer cell-derived tumour necrosis factor-alpha mediates the tumour growth-promoting response in macrophages by up-regulating the colony-stimulating factor-1 pathway. Cancer Research. 2007 Feb 1; 67(3): 1038-45. doi:10.1158/0008-5472.CAN-06-2295
14. Thaker AI. Shaker A. Rao MS. Ciorba MA. Modelling colitis-associated cancer with azoxymethane (AOM) and dextran sulphate sodium (DSS). Journal of Visualized Experiments. 2012 Sep 11;(67):4100. doi:10.3791/4100
15. Kusmardi K. Wiyarta E. Estuningtyas A. Sahar N. Midoen YH. Tedjo A. Potential of Phaleria macrocarpa Leaves Ethanol Extract to Upregulate the Expression of Caspase-3 in Mouse Distal Colon after Dextran Sodium Sulphate Induction. Pharmacognosy Journal. 2021;13(1):23-29. doi: 05530/pj.2021.13.4
16. Kusmardi K. Wiyarta E. Estuningtyas A. Sahar N. Midoen YH. Tedjo A. Pakpahan A. Potential Inhibition by Phaleria macrocarpa Leaves Ethanol Extract on Ki-67 Expression in Distal Colon Mouse. Pharmacognosy Journal. 2021;13(2):443-449. doi: 10.5530/pj.2021.13.56
17. Rustamadji P. Wiyarta E. Bethania KA. Kusmardi K. Potential of AKT2 expression as a predictor of lymph-node metastasis in invasive breast carcinoma of no special type. Journal of Pathology and Translational Medicine. 2021 Jul;55(4):271-278. doi: 10.4132/jptm.2021.04.26
18. Maharani R. Kusmardi. Elya B. Inhibitory activity goblet depletion and focal inflammatory phaleria macrocarpha leaves ethanol extract on crypta mouse after dextran sodium sulphate induction. International Journal of PharmTech Research. 2019; 12(1): 37-48. doi:10.20902/IJPTR.2019.120106
19. Ramos-Vara JA. Technical aspects of immunohistochemistry. Veterinary pathology. 2005; 4: 405-26. doi: 10.1354/vp.42-4-405
20. Amalia AW. Kusmardi. Elya B. Arsianti A. Inhibition of carcinogenesis by seed and soybean meal extract in colon of mice: Apoptosis and dysplasia. Asian Journal of Pharmaceutical and Clinical Research. 2017 Apr 1; 10(4): 123-128. doi:10.22159/ajpcr. 2017.v10i4.16276
21. Kusmardi K. Wiyarta E. Rusdi NK. Maulana AM. Estuningtyas A. Sunaryo H. The potential of lunasin extract for the prevention of breast cancer progression by upregulating E-Cadherin and inhibiting ICAM-1. F1000 Research. 2021 Sep 8; 10:902. doi: 10.12688/f1000research.55385.1
22. Fardet L. Galicier L. Lambotte O. Marzac C. Aumont C. Chahwan D. Coppo P et al Development and validation of the HScore, a score for the diagnosis of reactive hemophagocytic syndrome. Arthritis & Rheumatology. 2014 Sep; 66(9): 2613-20. doi:10.1002/art.38690
23. Hayden MS. Ghosh S. Regulation of NF-κB by TNF Family Cytokines. Seminars in Immunology. 2014 Jun; 26(3): 253–266. doi:10.1016/j.smim.2014.05.004
24. Oeckinghaus A. Ghosh S. The NF-κB family of transcription factors and its regulation. Cold Spring Harbor Perspectives in Biology. 2009 Oct; 1(4): a000034. doi:10.1101/cshperspect.a000034
25. Yang LG. Song ZX. Yin H. Wang YY. Shu GF. Lu HX. Wang SK et al Low n-6/n-3 pufa ratio improves lipid metabolism, inflammation, oxidative stress and endothelial function in rats using plant oils as n-3 fatty acid source. Lipids. 2016 Jan; 51(1): 49-59. doi:10.1007/s11745-015-4091-z
26. Kirpich IA. Feng W. Wang Y. Liu Y. Beier JI. Arteel GE. Cameron K et al Ethanol and dietary unsaturated fat (corn oil/linoleic acid enriched) cause intestinal inflammation and impaired intestinal barrier defence in mice chronically fed alcohol. Alcohol. 2013 May;47(3):257-64. doi: 10.1016/j.alcohol.2013.01.005