Author(s): Haider Qassim Raheem, Ehasn F. Hussein, Ahmed Hameed Rasheed

Email(s): haiderbio412@gmail.com , ehsan.f.hussein@gmail.com , alhly612@gmail.com

DOI: 10.52711/0974-360X.2022.00529   

Address: Haider Qassim Raheem1, Ehasn F. Hussein2, Ahmed Hameed Rasheed3
1,3DNA Research Center, University of Babylon, Hilla, Babylon, Iraq.
2College of Medicine of Hamorabi, University of Babylon, Hilla, Babylon, Iraq.
*Corresponding Author

Published In:   Volume - 15,      Issue - 7,     Year - 2022


ABSTRACT:
Pseudomonas aeruginosa, a Gram-negative human pathogen, P. aeruginosa is lone of the furthermost common hospital pathogens also is a chief concern, particularly in immune-compromised patients. The purpose of this study was to identify phenotypic and genotypic antibiotic resistance in Pseudomonas aeruginosa isolated from wound infection The bacterial isolates (30) were obtained from patients admitted to Mirjan Medical City in Babylon, Iraq (burns, wound unit). was identified biochemically and morphologically, and the isolates were subjected to standard bacteriological culturing processes on blood and MacConkey agar plates for 24-48 hours at 37oC for isolation and purification, Viteck 2 compact system confirmed the isolates and antibacterial sensitivity as well. These findings revealed that P.aeruginosa has a high rate of penicillin resistance, with a resistance rate of (100percent ) isolates. Advanced resistance to cephalosporin antibiotics was also found in resistant isolates of Cefoxitin, Ceftriaxone (75%), Ceftazidime, and cefepime (85%). For carbapenem antibiotics, had a high resistance rate (90percent). Aminoglycosides have variable resistance to Amikacin (60percent), Gentamicin (70 percent), and tobramycine resistant (90%). The findings revealed that all Pseudomonas aeruginosa isolates tested positive for Class 1,2 Integron resistance genes. with positive results (35%) for Int1 and 35% for Int2 (25%). This study found that P.aeruginosa has a high rate of resistance to Penicillins, Cephalosporin, Carbapenem and Aminoglycosides antibiotics.


Cite this article:
Haider Qassim Raheem, Ehasn F. Hussein, Ahmed Hameed Rasheed. Class 1,2 Integron Genes Distribution in Pseudomonas aeruginosa Isolated from Clinical Specimens. Research Journal of Pharmacy and Technology. 2022; 15(7):3165-8. doi: 10.52711/0974-360X.2022.00529

Cite(Electronic):
Haider Qassim Raheem, Ehasn F. Hussein, Ahmed Hameed Rasheed. Class 1,2 Integron Genes Distribution in Pseudomonas aeruginosa Isolated from Clinical Specimens. Research Journal of Pharmacy and Technology. 2022; 15(7):3165-8. doi: 10.52711/0974-360X.2022.00529   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-7-51


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