Haider Qassim Raheem, Ehasn F. Hussein, Ahmed Hameed Rasheed
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Haider Qassim Raheem1, Ehasn F. Hussein2, Ahmed Hameed Rasheed3
1,3DNA Research Center, University of Babylon, Hilla, Babylon, Iraq.
2College of Medicine of Hamorabi, University of Babylon, Hilla, Babylon, Iraq.
Volume - 15,
Issue - 7,
Year - 2022
Pseudomonas aeruginosa, a Gram-negative human pathogen, P. aeruginosa is lone of the furthermost common hospital pathogens also is a chief concern, particularly in immune-compromised patients. The purpose of this study was to identify phenotypic and genotypic antibiotic resistance in Pseudomonas aeruginosa isolated from wound infection The bacterial isolates (30) were obtained from patients admitted to Mirjan Medical City in Babylon, Iraq (burns, wound unit). was identified biochemically and morphologically, and the isolates were subjected to standard bacteriological culturing processes on blood and MacConkey agar plates for 24-48 hours at 37oC for isolation and purification, Viteck 2 compact system confirmed the isolates and antibacterial sensitivity as well. These findings revealed that P.aeruginosa has a high rate of penicillin resistance, with a resistance rate of (100percent ) isolates. Advanced resistance to cephalosporin antibiotics was also found in resistant isolates of Cefoxitin, Ceftriaxone (75%), Ceftazidime, and cefepime (85%). For carbapenem antibiotics, had a high resistance rate (90percent). Aminoglycosides have variable resistance to Amikacin (60percent), Gentamicin (70 percent), and tobramycine resistant (90%). The findings revealed that all Pseudomonas aeruginosa isolates tested positive for Class 1,2 Integron resistance genes. with positive results (35%) for Int1 and 35% for Int2 (25%). This study found that P.aeruginosa has a high rate of resistance to Penicillins, Cephalosporin, Carbapenem and Aminoglycosides antibiotics.
Cite this article:
Haider Qassim Raheem, Ehasn F. Hussein, Ahmed Hameed Rasheed. Class 1,2 Integron Genes Distribution in Pseudomonas aeruginosa Isolated from Clinical Specimens. Research Journal of Pharmacy and Technology. 2022; 15(7):3165-8. doi: 10.52711/0974-360X.2022.00529
Haider Qassim Raheem, Ehasn F. Hussein, Ahmed Hameed Rasheed. Class 1,2 Integron Genes Distribution in Pseudomonas aeruginosa Isolated from Clinical Specimens. Research Journal of Pharmacy and Technology. 2022; 15(7):3165-8. doi: 10.52711/0974-360X.2022.00529 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-7-51
1. Rasmussen BS, Christensen N, Sorensen J, Rosenvinge FS, Kolmos HJ, Skov MN. Outbreak of pseudomonas aeruginosa bacteraemia in a haematology department. Danish Medical Journal. 2015;62(4):A5040-.
2. Varshan R, Prakasam G. Detection of bla VIM gene encoding Metallo Beta Lactamase resistance among clinical isolates of Pseudomonas aeruginosa. Research Journal of Pharmacy and Technology. 2016;9(9):1465-8. doi.org/10.5958/0974-360X.2016.00284.5
3. Jebaraj AS, Gopinath P. Antibacterial Activity of Honey Against Clinical Isolates of Pseudomonas aeruginosa. Research Journal of Pharmacy and Technology. 2016;9(8):1174-6. doi.org/10.5958/0974-360X.2016.00224.9
4. Devi CS, Mohanasrinivasan V, Das D, Sharma P, Vaishnavi B, Naine SJ, Dhamodaran D. Optimization Studies on Nattokinase enzyme Production by a Mutant Strain Pseudomonas aeruginosa CMSS Isolated From Bovine Milk. Research Journal of Pharmacy and Technology.2014;7(11):1297-9.
5. Bharadwaj S, Teotia UV, Singh K, Sharma R, Singh Y. Effect of antibiotic on various microorganisms isolated from nosocomial infected patients in general hospital. Research Journal of Pharmacy and Technology. 2014;7(4):408-14.
6. Shanker K, Krishna Mohan G, Bhagavan Raju M, Divya L, Sanjay B. Efficacy of leaves extract of Acacia nilotica against Pseudomonas aeruginosa with reference to Disc diffusion method. Res J Pharmacognosy and Phytochem. 2014;6(2):96-8.
7. Marnoor SA. A Review on Antimicrobial Resistance and Role of Pharmacist in tackling this Global Threat. Research Journal of Pharmaceutical Dosage Forms and Technology. 2017;9(4):143-6.doi.org/10.5958/0975-4377.2017.00023.4
8. Abbas HA, Serry FM, EL-Masry EM. Combating Pseudomonas aeruginosa biofilms by potential biofilm inhibitors. Asian Journal of Research in Pharmaceutical Science. 2012;2(2):66-72.
9. A-Ameri GA, Alkolaibe AM, Ahmed M, Al-kadassy MN, Abdubaset AZ. Determination the efficiency of camel's urine against multi-drugs resistant Pseudomonas aeruginosa (MDRPA), isolated from effected burns, wounds and ears. Asian J. Pharm. Res. 2015;5(1):1-9.doi.org/10.5958/2231-5691.2015.00001.5
10. Mac Faddin JF. Biochemical tests for identification of medical bacteria.
11. Varshitha A, Gopinath P.Detection of blaTEM-1 gene for ESBL Production among Clinical isolates of Pseudomonas aeruginosa. Research Journal of Pharmacy and Technology.2016;9(10):1623-5.doi.org/10.5958/0974-360X.2016.00323.1
12. PEYM AnI AM, FARA JnIA SA, Nahaei MR, Sohrabi N, Abbasi L, Ansarin K, Azhari F. Prevalence of class 1 integron among multidrug-resistant Acinetobacter baumannii in Tabriz, northwest of Iran. Pol J Microbiol. 2012;61(1):57-60.
13. Al-Shwaikh RM, Alornaaouti AF. Detection of tox A gene in Pseudomonas aeruginosa that isolates from different clinical cases by using PCR. Ibn AL-Haitham Journal For Pure and Applied Science. 2018:26-30.
14. Al-Taie LH, Hassan S, Al-Mayah KS, Talib S. Isolation and identification of bacterial burn wound infection and their sensitivity to antibiotics. Al-Mustansiriyah J Sci. 2014;25(2):17-24.
15. Abbas HA, Serry FM, El-Masry EM. Synergic interaction between antibiotics and the artificial sweeteners xylitol and sorbitol against Pseudomonas aeruginosa biofilms. Asian J. Pharm. Res. 2012;2(4):129-31.
16. ÇİÇek AÇ, Düzgün AÖ, Saral A, Kayman T, Çİzmecİ Z, Balcı PÖ, Dal T, Fırat M, Tosun İ, Alıtntop YA, Çalışkan A. Detection of class 1 integron in Acinetobacter baumannii isolates collected from nine hospitals in Turkey. Asian Pacific journal of tropical biomedicine. 2013;3(9):743-7. doi.org/10.1016/S2221-1691(13)60149-5
17. Poole K. Efflux-mediated multiresistance in Gram-negative bacteria. Clinical Microbiology and infection. 2004;10(1):12-26. doi.org/10.1111/j.1469-0691.2004.00763.x
18. Valot B, Guyeux C, Rolland JY, Mazouzi K, Bertrand X, Hocquet D. What it takes to be a Pseudomonas aeruginosa? The core genome of the opportunistic pathogen updated. PloS one. 2015 ;10(5):e0126468. 5doi.org/10.1371/journal.pone.0126468
19. Williams H, Campbell L, Crompton RA, Singh G, McHugh BJ, Davidson DJ, McBain AJ, Cruickshank SM, Hardman MJ. Microbial host interactions and impaired wound healing in mice and humans: defining a role for BD14 and NOD2. Journal of Investigative Dermatology. 2018;138(10):2264-74. doi.org/10.1111/j.1469-0691.2004.00763.x
20. Abraham S. Molecular characterization of commensal and pathogenic Escherichia coli that colonise human urinary tract and the porcine gastrointestinal tract and the development of whole cell biosensors to evaluate bacteriocin mediated bacterial interactions. 2011.
21. Mahdian S, Sadeghifard N, Pakzad I, Ghanbari F, Soroush S, Azimi L, Rastegar-Lari A, Giannouli M, Taherikalani M. Acinetobacter baumannii clonal lineages I and II harboring different carbapenem-hydrolyzing-β-lactamase genes are widespread among hospitalized burn patients in Tehran. Journal of Infection and Public Health. 2015;8(6):533-42. doi.org/10.1016/j.jiph.2015.04.030
22. Somayeh, Mahdian, Sadeghifard Nourkhoda, Pakzad Iraj, Ghanbari Fatemeh, Soroush Setareh, Azimi Lila, Rastegar Lari Abdolaziz, Giannouli Maria, and Taherikalani Morovat. Acinetobacter baumannii clonal lineages I and II harboring different carbapenem-hydrolyzing-beta-lactamase genes are widespread among hospitalized burn patients in Tehran. 2015: 533-542.
23. Roy Chowdhury P, Stokes H, Labbate M. Integrons: antibiotic resistance evolution and beyond. Bacterial integrative mobile genetic elements. 2013 doi.org/10.1007/978-1-4614-7780-8_4