Author(s): Adnan Ali Hammad, Ayman A. Farrag, Tarek M. Abdelghany, Amr A. El-Waseif, Saleem Obaid Gatia Almawla

Email(s): adad15693@gmail.com

DOI: 10.52711/0974-360X.2022.00528   

Address: Adnan Ali Hammad1,2, Ayman A. Farrag2,3, Tarek M. Abdelghany2, Amr A. El-Waseif2, Saleem Obaid Gatia Almawla4
1Anbar Education Directorate, Iraqi Ministry of Education, Anbar, Iraq.
2Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
3Al-Azhar Center for Fermentation Biotechnology and Applied Microbiology Al-Azhar University, 11884 Nasr City, Cairo, Egypt.
4Alanbar general Health directorate, Ministry of health, Anbar, Iraq.
*Corresponding Author

Published In:   Volume - 15,      Issue - 7,     Year - 2022


ABSTRACT:
Plant extracts promise natural sources of antivirulence chemicals thanks to their broad availability and various phytochemical antivirulence components. The main antivirulence components of plants are essential oils. 130 isolates were obtained from neonates (=1 year old) from February, 2020 to February, 2021. All studied isolates were diagnosed by traditional methods like biochemical tests, Vitek-2 system, and 16srRNA using conventional PCR. Profile of antibiotics resistance ratio for Streptococcus agalactiae were Penicillin 20%, Clindamycin 68%, Chloramphenicol 46%, Tetracycline 82%, Erythromycin 100%, Imipenem 12%, and Ciprofloxacillin 26.47%. All genes (Cyl E, Spb1, and bibA) were detected by conventional PCR and the molecular frequency showed 64% of strains positive for Cyl E gene and 56% of strains harbored bibA genes, while 48% of Streptococcus agalactiae contain Spb1 gene. This research showed the coexistence of bla Cyl E, Spb 1 and bibA genes in 48% strains of Streptococcus agalactiae. Extraction of clove extract was carried out using methanol and it analysed using GC-MS to detect active compounds, such as Eugenol (60.10%). The effect of clove extract against biofilm formation, haemolysin, and protease activity was significant (p-value < 0.01).


Cite this article:
Adnan Ali Hammad, Ayman A. Farrag, Tarek M. Abdelghany, Amr A. El-Waseif, Saleem Obaid Gatia Almawla. Correlation Study of Alcoholic Clove extract against Streptococcus agalactiae virulence factors isolated from Neonatal patients. Research Journal of Pharmacy and Technology. 2022; 15(7):3159-4. doi: 10.52711/0974-360X.2022.00528

Cite(Electronic):
Adnan Ali Hammad, Ayman A. Farrag, Tarek M. Abdelghany, Amr A. El-Waseif, Saleem Obaid Gatia Almawla. Correlation Study of Alcoholic Clove extract against Streptococcus agalactiae virulence factors isolated from Neonatal patients. Research Journal of Pharmacy and Technology. 2022; 15(7):3159-4. doi: 10.52711/0974-360X.2022.00528   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-7-50


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