Author(s): Maysoon Khaleefa Abbas, Fatima Rammadan Abdul, Khetam H. Rasool

Email(s): maysoon.bio2005@uomustansiriyah.edu.iq , mht1695@uomustansiriyah.edu.iq

DOI: 10.52711/0974-360X.2022.00522   

Address: Maysoon Khaleefa Abbas1, Fatima Rammadan Abdul2, Khetam H. Rasool3
1,2,3Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
*Corresponding Author

Published In:   Volume - 15,      Issue - 7,     Year - 2022


ABSTRACT:
The current work aimed to study the propagation of Staphylococcus aureus and Candida sp. and. in oral cavity of the human, and their effective on innetimmune response and to describe the biochemical &kinetic properties of PLB from the Staphylococcus aureus. and asporogenous yeast C. tropicalis. The study included 25 patients, ages (12-65) years, with periodontal diseases and at least two sites with probing depth = 3 mm. Participants' data were assessed individually. A sample is taken from the oral cavity (mucous membrane, tongue and cheek) with a sterile swab. Among all the patients studied, (16) 64% showed Staphylococcus in the oral cavity while Streptococcus sp (3) 12% showed candidiasis in the oral cavity and (5) 20 % of other bacteria(1) 4% in the oral cavity. The effect of microbial inflammation on the multidirectional secretion of interleukin (IL) -10, IL-6 has been studied in patients with oral cavity infections. Samples and wash swabs were taken from 25 healthy patients and 25 patients. The supernatant fractions of IL-6 and IL-10 were examined by commercial ELISAs. Analysis of interleukin levels in the two groups with the Mann-Whitney test.IL-6 levels various significantly between the patients, the control group, and the control groups. Median levels of IL-10 in both groups were uniformly low but the mean levels were not statistically different. The observed change in bacterial flora that is characteristic of stomatitis does not enhance or suppress oral levels of IL-6 and IL-10. Staphylococcus aureus, and Candida tropicalis have been isolated oral cavity and under the periodontal sinuses infection. It was filtered 2.5 fold at40 ºC from a culture broth and has an estimated molecular mass of 50kDa.The enzyme was two pH targets, one acid (pH 4.0) and other alkaline (pH 7.5). At acidic pH the enzyme showed a higher affinity for all tested phospholipids without metal ions while its affinity affected by the presence of metal ions at alkaline conditions and the enzyme activity inhibited by Fe3+ and Hg2+.


Cite this article:
Maysoon Khaleefa Abbas, Fatima Rammadan Abdul, Khetam H. Rasool. Immunological and Enzymatic study of Staphylococcus aureus Bacteria and fungi isolated from oral cavity. Research Journal of Pharmacy and Technology. 2022; 15(7):3119-4. doi: 10.52711/0974-360X.2022.00522

Cite(Electronic):
Maysoon Khaleefa Abbas, Fatima Rammadan Abdul, Khetam H. Rasool. Immunological and Enzymatic study of Staphylococcus aureus Bacteria and fungi isolated from oral cavity. Research Journal of Pharmacy and Technology. 2022; 15(7):3119-4. doi: 10.52711/0974-360X.2022.00522   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-7-44


REFERENCES:
1.    Bander, K. I., & Hamad, T. A. Prevalence of Vaginal Candidiasis among women and Diagnosis of Candida species from vaginal infection in Kirkuk city. Tikrit J. of Pure Sci., 20(4), 5-15(2018).‏
2.    Colombo, A. L., de Almeida, J. N., Slavin, M. A., Chen, S. C., & Sorrell, T. C. Candida and invasive mould diseases in non-neutropenic critically ill patients and patients with haematological cancer. The Lan. Infec. Dis., 17(11), e344-e356(2017).
3.    Kaur, R., Dhakad, M. S., Goyal, R., & Kumar, R. Emergence of non-albicans Candida species and antifungal resistance in intensive care unit patients. Asian Pacific J. of Tro. Biom., 6(5), 455-460(2016).‏
4.    Shaker, D. S., Ishak, R. A., Ghoneim, A., and Elhuoni, M. A. Nanoemulsion: A review on mechanisms for the transdermal delivery of hydrophobic and hydrophilic drugs. Scientia Pharmaceutica, 87(3), 17(2019).‏
5.    Bolen, A. L., Naren, A. P., Yarlagadda, S., Beranova-Giorgianni, S., Chen, L., Norman, D., Tigyi, G. The phospholipase A1 activity of lysophospholipase AI links platelet activation to LPA production during blood coagulation. Journal of lipid research, 52(5), 958-970.‏(2011).
6.    Ma, Z., & Turk, J. The molecular biology of the group VIA Ca2+-independent phospholipase A2(2001).‏
7.    Ishiguro, S., Kawai-Oda, A., Ueda, J., Nishida, I., & Okada, K. the defective in anther dehiscence1 gene encodes a novel phospholipase A1 catalyzing the initial step of jasmine acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis. The Plant Cell, 13(10), 2191-2209(2001).‏  
8.    Wainszelbaum, M., isola, E., wilkowsky, S., cannata, J., florin-christensen, J., & florin-christensen, M. Lysosomal phospholipase A1 in Trypanosomacruzi: an enzyme with a possible role in the pathogenesis of Chagas’ disease. Biochemical J., 355(3), 765-770. (2001).‏
9.    Fujino, S., Akiyama, D., Akaboshi, S., Fujita, T., Watanabe, Y., &Tamai, Y. Purification and characterization of phospholipase B from Candida utilis. Bioscience, biotechnology and biochemistry, 70(2), 377-386(2006)..‏
10.    Feldman, M., Sionov, R. V., Mechoulam, R., & Steinberg, D. Anti-Biofilm Activity of Cannabidiol against Candida albicans. Microorganisms, 9(2), 441(2021).‏
11.    Zuza-Alves, D. L., Silva-Rocha, W. P., & Chaves, G. M. An update on Candida tropicalis based on basic and clinical approaches.Frontiers in microbiology, 8, 1927(2017).
12.    Kong, E. F. Clinical and Therapeutic Implications of Biofilm-Associated Fungal-Bacterial Interactions: Candida albicans and Staphylococcus aureus (Doctoral dissertation, University of Maryland, Baltimore(2017).‏‏
13.    Collee, J. G.; Fracer, A. G.; Marmion, B. P. and Simmon, A. Practical Medical Microbiology (Mackie and MacCartenyeds). 14th edition. Churchill Livingstone, Singapore(1996).
14.    Abdul, F. R. Evaluation of some Virulence Factors, Hemagglutination and Agglutination of Antigens of Acinetobacter baumannii Isolated From Clinical Samples. Journal of Global Pharm. Technology; 10 (03):200-208.(2018).
15.    Oishi, H., Morimoto, T., Watanabe, Y., &Tamai, Y. Purification and characterization of phospholipase B from Kluyveromyceslactis, and cloning of phospholipase B gene. Bioscience, biotechnology, and biochemistry, 63(1), 83-90(1999).‏
16.    Naglik, J. R., Rodgers, C. A., Shirlaw, P. J., Dobbie, J. L., Fernandes-Naglik, L. L., Greenspan, D., Challacombe, S. J. Differential expression of Candida albicans secreted aspartyl proteinase and phospholipase B genes in humans correlates with active oral and vaginal infections. The Journal of infectious diseases, 188(3), 469-479(2003).‏
17.    Cuesta, A. I., Jewtuchowicz, V., Brusca, M. I., Nastri, M. L., & Rosa, A. C. Prevalence of Staphylococcus spp and Candida spp in the oral cavity and periodontal pockets of periodontal disease patients. Acta Odontológica Latino Americana, 23(1), 20-26(2010).‏
18.    Abbas, Maysoon. K., Hussain, S. S., Noor, A. H. A., and Khadhom, I. Immunological and Molecular Study of Toll-Like Receptor-4 in Patients with Urinary Tract Infections. Annals of Tropical Medicine and Health, 23, 23-934(2020).‏
19.    Hamdy, A. N., Abbas, Maysoon. K., Khudhair, M. A., and Tektook, N. K. Interactions between Interleukin-6 and MDA in Women with Preeclampsia. Systematic Reviews in Pharmacy, 11(3) (2020).‏
20.    Abbas, M. K. " Changes in Immune Markers for Prostate Cancer Patients Pre and Post-operation". College of Science, University of Al-Mustansiriyah, Iraqi J. of Science, 55,3B:1218-1231.2014.

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