Author(s): Agung Krismariono, Yunita Purwaningsih

Email(s): agung-k@fkg.unair.ac.id.

DOI: 10.52711/0974-360X.2022.00518   

Address: Agung Krismariono1, Yunita Purwaningsih2
1Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
2Student of Periodontic Residency Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya.
*Corresponding Author

Published In:   Volume - 15,      Issue - 7,     Year - 2022


ABSTRACT:
The most common case of periodontitis is chronic periodontitis. Chronic periodontitis is characterized by the destruction of junctional epithelium caused by Porphyromonas gingivalis. Junctional epithelial attached to the tooth are mediated by laminin-5. The cleavage of the ?2 chain of laminin-5 causes junctional epithelium destruction. The aim of this study is to observe the expression of laminin-5?2 in 1% curcumin-treated rats with periodontitis. Forty-eight periodontitis rats model were divided into 2 groups. The first is control group consisted of twenty-four periodontitis rats model exposed with Porphyromonas gingivalis. The second is treatment group consisted of twenty-four periodontitis rats model exposed to Porphyromonas gingivalis and 1% curcumin administration. Each group was observed at 24 hours on days-7 and days-14. Junctional epithelium samples in sagittal direction were examined by immunohistochemistry and were statistically analyzed using t-test (a=0.05). The result showed that laminin-5?2 expression in treatment group was significantly lower than the control group on days-7 and days-14. In treatment group, laminin-5?2 expression at 24 hours was significantly lower than days-7 and days-14. In treatment group, laminin-5?2 expression did not experience a significant difference in between days-7 and days-14. This study showed that 1% curcumin can reduce laminin-5?2 expression in periodontitis.


Cite this article:
Agung Krismariono, Yunita Purwaningsih. The Influence of 1% Curcumin on Laminin -5γ2 In Periodontitis Condition. Research Journal of Pharmacy and Technology. 2022; 15(7):3096-0. doi: 10.52711/0974-360X.2022.00518

Cite(Electronic):
Agung Krismariono, Yunita Purwaningsih. The Influence of 1% Curcumin on Laminin -5γ2 In Periodontitis Condition. Research Journal of Pharmacy and Technology. 2022; 15(7):3096-0. doi: 10.52711/0974-360X.2022.00518   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-7-40


REFERENCES:
1.    Camabala AD. Gajendran P. Role of Interleukin 1 in Gingival Tissues in Chronic Periodontitis -A Review. Research J. Pharm. and Tech. 2017; 10(9): 3185-3187. doi: 10.5958/0974-360X.2017.00566.2
2.    Sanjeevi J. Gajendran P. Role of Interleukin 1 in serum in Chronic Periodontitis -A Review. Research J. Pharm. and Tech. 2017; 10(11): 4090-4092. doi: 10.5958/0974-360X.2017.00741.7
3.    Inayathulla. Goudanavar P. Acharya A.  Development and Evaluation of In-situ gel containing Linezolid and Diclofenac Sodium in the treatment of Periodontitis. Asian J. Pharm. Tech. 2020; 10(1):20-26. doi: 10.5958/2231-5713.2020.00005.7
4.    Pavithra RS. Varghese S. Gingival Tissue Level of Interleukin-1 in Diabetic Patient with Chronic Periodontitis. Research J. Pharm. and Tech. 2017; 10(12): 4442-4444. doi: 10.5958/0974-360X.2017.00818.
5.    Prithi R. Geetha RV. Static Effects of Fruits on Periodontitis. Research J. Pharm. and Tech. 2014; 7(3): 365-367.
6.    Gayathri. M. Dr. Gheena. S. Gopinath. Comparison of the aerobic microbes in supragingival plaque of chronic and aggressive periodontitis patients. Research J. Pharm. and Tech. 2016; 9(9):1363-1366. doi: 10.5958/0974-360X.2016.00261.4
7.    Newman MG. Takei H. Klokkevold PR. Carranza FA. Newman. Carranza’s clinical periodontology. 11th ed. Philadelphia London New-York. WB.Saunders Co. 2012;  p: 17-31, 96-103, 54-164, 336-350.
8.    Feng Z. Weinberg A. Role of bacteria in health and disease of periodontal tissues. Periodontology 2000. 2006; 40: 50-76
9.    Thorup AK. Dabelsteen E. Schou S.  Gil SG, Carter WG and Reibel J. Differential expression of integrins and laminin-5 in normal oral epithelia. APMIS. 1997; 105: 519-530. doi: 10.1111/j.1699-0463.1997.tb05049.x  
10.    Hormia M. Sahlberg CI. Airenne T. The epithelium-tooth interface - a basal lamina rich in laminin-5 and lacking other known laminin isoforms. J Dent Res. 1998; 77: 1479-1484. doi: 10.1177/00220345980770070201
11.    Mullen LM. Richards DW. Quaranta V. Evidence that laminin-5 is a component of the tooth surface internal basal lamina supporting epithelial cell adhesion. J Periodontal Res. 1999; 34 (1):16-24. doi: 10.1111/j.1600-0765.1999.tb02217.x
12.    Hormia M. Owaribe K. Virtanen I. The dento-epithelial junction: cell adhesion by type I hemidesmosomes in the absence of a true basal lamina. J Periodontol. 2001; 72:788-797. doi: 10.1902/jop.2001.72.6.788
13.    Oksanen J. Sorokin LM. Virtanen I.  Hormia M. The junctional epithelium around murine teeth differs from gingival epithelium in its basement membrane composition. J Dent Res. 2001; 80: 2093-2097. doi: 10.1177/00220345010800121401
14.    Pollanen MT. Salonen JI. Uitto VJ. Structure and function of the tooth-epithelial interface in health and disease. Periodontology 2000. 2003; 31: 12-31. doi: 10.1034/j.1600-0757.2003.03102.x
15.    Shimono M. Biological characteristics of the junctional epithelium. Journal of Electron Microscopy. 2003; 52 (6): 627-639. doi:10.1093/JMICRO/52.6.627.
16.    Kinumatsu T. Hashimoto S. Muramatsu T. Sasaki H. Jung HS. Yamada S. Shimono M. Involvement of laminin and integrins in adhesion and migration of junctional epithelium cells. J Periodont Res. 2009; 44: 13-20. doi: 10.1111/j.1600-0765.2007.01036.x
17.    Sugisawa M. Masaoka T. Enokiya Y. Muramatsu T. Hashimoto S. Yamada S. Shimono M. Expression and function of laminin and integrins on adhesion/migration of primary culture cells derived from rat oral epithelium. J Periodont Res. 2010; 45: 284-291. doi: 10.1111/j.1600-0765.2009.01231.x
18.    Colognato H. Yurchencho PD. Form and function: the laminin family of heterotrimers. Developmental Dynamics. 2000. 218: 213-234. doi: 10.1002/(sici)1097-0177(200006)218:2<213::aid-dvdy1>3.0.co;2-r
19.    Emingil G. Kuula H. Pirila E. Atilla G. Sorsa T. Gingival crevicular fluid laminin-5 γ2-chain levels in periodontal disease J Clin Periodontol. 2006; 33: 462-468. doi: 10.1111/j.1600-051x.2006.00933.x
20.    WHO. Traditional medicine strategy 2002-2005. 2002; 1-3. https://apps.who.int/iris/handle/10665/67163
21.    Aggarwal BB. Bhatt ID. Ichikawa H. Ahn KS. Sethi G. Sandur SK. Natarajan C. Seeram N. Shishodia S. Curcumin: biological and medicinal properties. 2006; p: 297-340
22.    Chattopadhyay I. Biswas K. Bandyopadhyay U. Banerjee RK. Turmeric and curcumin: biological actions and medicinal applications. Current Science. 2004; 87 (1): 44-49
23.    Jurenka JS. Anti-inflammatory properties of curcumin. a major constituent of curcuma longa. A review of preclinical and clinical research. Alternative Medicine Review. 2009; 14 (2): 141-153
24.    Sha AM. Garib BT. Antibacterial Effect of Curcumin against Clinically Isolated Porphyromonas gingivalis and Connective Tissue Reactions to Curcumin Gel in the Subcutaneous Tissue of Rats. Research Article of BioMed Research International. 2019; (2019) doi.org/10.1155/2019/6810936
25.    Zhou T. Chen D. Li Q. Sun X. Song Y. Wang C. Curcumin inhibits inflammatory response and bone loss during experimental periodontitis in rats. Acta Odontol Scand. 2013; 71(2): 349-56. doi: 10.3109/00016357.2012.682092
26.    Guimaraes MR. Coimbra LS. de Aquino SG. Spolidorio LC. Kirkwood KL. Rossa C. Potent anti-inflammatory effects of systemically administered curcumin modulate periodontal disease in vivo. J Periodont Res. 2011; 46: 269-279. doi: 10.1111/j.1600-0765.2010.01342.x
27.    Suhag A. Dixit J. Dhan P. Role of curcumin as a subgingival irrigant: A pilot study. Qiuntessence Journals. 2007; 4 (2):115-121
28.    Sreedhar A. Sarkar I. Rajan P. Pai J. Malagi S. Kamath V. Barmappa R. Comparative evaluation of the efficacy of curcumin gel with and without photo activation as an adjunct to scaling and root planing in the treatment of chronic periodontitis: A split mouth clinical and microbiological study. J of Natural Science, Biology and Medicine. 2015; 6: 102-109. doi: 10.4103/0976-9668.166100
29.    Suzumura Y. Kameyama Y. Mizutant M. Kato M. Kondo K. Mabuchi R. Long junctional epithelium produced by application of bacterial protease in rats. J Periodont Res. 1989; 24: 217-221. doi: 10.1111/j.1600-0765.1989.tb02009.x
30.    Polak D. Wilensky A. Shapira L. Halabi A. Goldstein D.  Weiss EI and Haddad YH. 2009. Mouse model of experimental periodontitis induced by Porphyromonas gingivalis / Fusobacteriumnucleatum infection: bone loss and host response. J Clin Periodontol. 2009; 36: 406-410. doi: 10.1111/j.1600-051x.2009.01393.x
31.    Giannelli G. Falk-Marzillier J. Schiraldi O. Stetler-Stevenson WG. Quaranta V. Induction of cell migration by matrix metalloprotease-2 cleavage of laminin-5. Science. 1997; 277: 225-226
32.    Gagnoux PL. Allergra M. Spirito F. Pommeret O. Romero C. Ortonne JP. Meneguzzi G. The short arm of the laminin g2-chain plays a pivotal role in the incorporation of laminin 5 into the extracellular matrix and in cell adhesion. Journal of Cell Biology. 2001; 153: 835-849. doi: 10.1083/jcb.153.4.835
33.    Chen D. Nie M. Fan MW. Bian Z. Anti-inflammatory activity of curcumin in macrophages stimulated by lipopolysaccharides from Porphyromonas gingivalis. Pharmacology. 2008; 82 (4): 264-9. doi: 10.1159/000161127
34.    Kuo JJ. Chang HH. Tsai TH. Lee TY. Positive effect of curcumin on inflammation and mitochondrial dysfunction in obese mice with liver steatosis International Journal of Molecular Medicine. 2012; (30): 673-679. doi: 10.3892/ijmm.2012.1049
35.    Philip S. Kundu GC. Osteopontin induces nuclear factor kappa B-mediated promatrix metalloproteinase-2 activation through IkB/IKK signaling pathways and curcumin (diferulolylmethane) down-regulates these pathways. J Biol Chem. 2003; 278:14487-14497. doi: 10.1074/jbc.m207309200
36.    Verma UP. Abhaya G. Disha S. Role of Curcuma longa in the Management of Gingivitis. International J of Public Health Science (IJPHS). 2018; 7 (3). 216-222. doi:10.11591/ijphs.v7i3.14830
37.    Mohammad CA. Efficacy of Curcumin Gel on Zinc, Magnesium, Copper, IL-1β, and TNF-α in Chronic Periodontitis Patients. Research Article. BioMed Research International. 2020; doi.org/10.1155/2020/8850926
38.    Livada R. Shiloah J. Tipton DA. Dabbous MK. The Potential Role of Curcumin in Periodontal Therapy: A Review of the Literature. J of Int Aca Periodon. 2017; 19(3):70-79.
39.    Anusha D. Chaly PE, Junaid M. Nijesh JE. Shivashankar K. Sivasamy S. Efficacy of A Mouthwash Containing Essential Oils and Curcumin As An Adjunct To Nonsurgical Periodontal Therapy Among Rheumatoid Arthritis Patients With Chronic Periodontitis: A Randomized Controlled Trial. Original Research. Indian J of Dental Research. 2019; 30 (4). 506-511. doi: 10.4103/ijdr.ijdr_662_17
40.    Kaur S. Sharma R. Sarangal V. Kaur N. Prashar P. Evaluation of Anti-Inflammatory Effects of Systemically Administered Curcumin, Lycopene and Piperine As An Adjunct To Scaling And Root Planing: A Clinical Study. Original Article. An. International Quarterly J Of Research in Ayurveda. 2017; 38 (2). 117-121. doi: 10.4103/ayu.ayu_63_17
41.    Lubis P. Nasution R Ilyas S, Ervina I, Primasari A. Effectiveness of 1% curcumin gel subgingival application on interleukin-6 levels in chronic periodontitis patients. International Journal of Applied Dental Sciences. 2020; 6(3): 513-516
42.    Susanto A. Amaliya A. SWN C. The Effect of 2% White Turmeric (Curcuma zedoaria) Gel as Adjunctive Therapy of Scaling and Root Planing Treatment toward the Interleukin 6 Level in Chronic Periodontitis. Int J of Chem Res. 2018; 11(08):182-87. doi.org/10.20902/ijctr.2018.1108
43.    Reddy SV. Suresh J. Hemant KS. Yadav. Singh A. A Review on Curcuma longa. Research J. Pharm. and Tech. 2012; 5(2):158-165.
44.    Prafulla S. Lata P. Priya R.Vidya S. Novel Curcumin Derivatives: Targeted for Anti-Inflammatory Activity. Asian J. Research Chem. 2019; 12(2):49-54. doi: 10.5958/0974-4150.2019.00011.7
45.    Das A. Roy A. Rajeshkumar S. Lakshmi T. Anti-inflammatory Activity of Turmeric Oil Mediated Silver Nanoparticles. Research J. Pharm. and Tech. 2019; 12(7):3507-3510. doi: 10.5958/0974-360X.2019.00596.1
46.    Ritmaleni. Sardjiman. Purwantini I. Antimicrobial Activity of Curcumin Analog PGV-6, HGV-6 and GVT-6. Research J. Pharm. and Tech. 2021; 14(2):599-604. doi: 10.5958/0974-360X.2021.00107.4.
47.    Xiao J. Yu XJ. Xie JL. Liu S. Li S.  Protective effect and related mechanisms of curcumin in rat experimental periodontitis. Research. Head & Face Medicine. 2018; 14:12 doi.org/10.1186/s13005-018-0169-1
48.    Krismariono A. The Decreasing of Nfκβ Level in Gingival Junctional Epithelium of Rat Exposed to Porphyromonas gingivalis With Application Of 1% Curcumin on Gingival Sulcus. Research Report.  Dental J. 2015; 48(1): 35–38. doi.org/10.20473/j.djmkg.v48.i1.p35-38
49.    Yamamoto Y. Gaynor RB. Therapeutic Potential of Inhibition of the NF-κβ Pathway in The Treatment of Inflammation and Cancer. J Clin Invest. 2001; 107 (2):135-142. doi: 10.1172/jci11914

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