Author(s): Sanakousar K. Patel, Arun K Shutter, Ria Patil, Archana Desangi, Vishalakshi Malali, Jyoti Patil, Sumangala Patil, Kusal K. Das, Prachi P. Parvatikar

Email(s): prachisandeepk@gmail.com

DOI: 10.52711/0974-360X.2022.00490   

Address: Sanakousar K. Patel1, Arun K Shutter2, Ria Patil3, Archana Desangi3, Vishalakshi Malali3, Jyoti Patil3, Sumangala Patil1, Kusal K. Das1, Prachi P. Parvatikar1*
1Laboratory of Vascular Physiology and Medicine, Department of Physiology, Shri B M Patil Medical College, Hospital and Research Centre, BLDE (DU), Vijayapura, Karnataka, India-586103.
2Cytxon Biosolution Pvt Ltd
3Karnataka State Akkamahadevi Women’s University, Vijayapura.
*Corresponding Author

Published In:   Volume - 15,      Issue - 7,     Year - 2022


ABSTRACT:
Objective: The phytochemical analysis of Terminalia chebula, Terminalia bellirica, Phyllanthus emblica fruit extract as well as to evaluate the potential synergistic activity of screened plant extracts for anti-oxidant, anti-inflammatory and anti-cancer activity on MCF-7 breast cancer cell line. Methodology: In present study phytochemical constituent from selected plant material was extracted in different solvents. Antioxidant activity was evaluated by DPPH and FRAP assay against standard antioxidant. MTT assay was performed to find out cytotoxic and anti-inflammatory effect on MCF- breast cancer cell line. Result: Phytochemical analysis of the performed which showed that alkaloids, phenols, flavonoids and saponins. In context of antioxidant assays almost selected extracts showed moderate activity but ethanol extract of Terminalia chebula, proven to be having significant antioxidant activity over all extracts. Anti-inflammatory activity of MCF-7 breast cancer cell line exhibited that good activity with IC50 value 228.82µg/ml. Whereas Methanol extract of Terminalia bellirica and Aqueous extract of Phyllanthus emblica shown 432.45µg and 259.02µg respectively. Conclusion: The present study concludes that in among three tested extracts, Ethanol extract of Terminalia chebula exhibited prominent anticancer activity against Breast cancer (MCF-7) with significant IC50 value 228.82 µg. However further studies are needed to find out the structural of bioactive compounds and investigate mechanisms of antioxidant and anticancer activities of the bioactive compounds in in-vivo animal study.


Cite this article:
Sanakousar K. Patel, Arun K Shutter, Ria Patil, Archana Desangi, Vishalakshi Malali, Jyoti Patil, Sumangala Patil, Kusal K. Das, Prachi P. Parvatikar. In-Vitro Antioxidant, Anti-Inflammatory and Cytotoxic effects of different Solvent Extraction Terminalia chebula, Terminalia billerica, Phyllanthus emblica. Research Journal of Pharmacy and Technology. 2022; 15(7):2940-4. doi: 10.52711/0974-360X.2022.00490

Cite(Electronic):
Sanakousar K. Patel, Arun K Shutter, Ria Patil, Archana Desangi, Vishalakshi Malali, Jyoti Patil, Sumangala Patil, Kusal K. Das, Prachi P. Parvatikar. In-Vitro Antioxidant, Anti-Inflammatory and Cytotoxic effects of different Solvent Extraction Terminalia chebula, Terminalia billerica, Phyllanthus emblica. Research Journal of Pharmacy and Technology. 2022; 15(7):2940-4. doi: 10.52711/0974-360X.2022.00490   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-7-12


REFERENCES:
1.    Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. International journal of cancer. 2015 Mar 1;136(5):E359-86. Breast cancer-trends in patients profiles attending tertiary cancer care centre in south India. Asian J Epidemiology 2012;5:103–3.
2.    Arjun Patidar, S.C. Shivhare, Umesh Ateneriya, Sonu Choudhary. A Comprehensive Review on Breast Cancer. Asian J. Nur. Edu. and Research 2(1): Jan.-March 2012; Page 28-32.
3.    Pratumvinit B, Srisapoomi T, Worawattananon P, Opartkiattikul N, Jiratchariyakul W, Kummalue T. In vitro antineoplastic effect of Ficus hispida L. plant against breast cancer cell lines. J Med Plants Res 2009;3:255–61.
4.    Preeti Tiwari, Rakesh K. Patel. Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Ashwagandharishta Prepared by Traditional and Modern Methods. Asian J. Pharm. Ana. 3(4): Oct. - Dec. 2013; Page 147-152.
5.    Krishna Kondragunta. V, Karuppuraj. V, Perumal. K. Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian J. Pharm. Ana. 2016; 6(4): 213-215.
6.    Priyanga Rangasamy, Vadakkenchery Salimudheen Hansiya, Palanisamy Uma Maheswari, Thamburaj Suman, Natesan Geetha. Phytochemical Analysis and Evaluation of In vitro Antioxidant and Anti-urolithiatic Potential of various fractions of Clitoria ternatea L. Blue Flowered Leaves. Asian J. Pharm. Ana. 2019; 9(2): 67-76.
7.    Rangarajan Narasimhan, Sathiyamoorthy. M. Phytochemical Screening and Antioxidant Studies in the Pulp Extracts of Cucurbita maxima. Asian J. Pharm. Res. 6(1): January -March, 2016; Page 01-04.
8.    Atoussi Ouidad, Chetehouna Sara, Derouiche Samir. Biological properties and Acute Toxicity Study of Copper oxide nanoparticles prepared by aqueous leaves extract of Portulaca oleracea (L). Asian J. Pharm. Res. 2020; 10(2):89-94.
9.    N. Naidu, G. Sudheer Kumar, K. Sivakrishna, K. Anjinaik, L. Praveen Kumar, G. Sneha. Anti microbial and antioxidant evolution of aqueous extract of Terminalia chebula using disc diffusion, H2O2 scavenging methods. Asian J. Res. Pharm. Sci. 2017; 7(2): 112-114
10.    Zhao T, Sun Q, Marques M, Witcher M. Anticancer properties of. Phyllanthus emblica.
11.    Umadevi P, Deepti K, Srinath I, Vijayalakshmi G, Tarakaramji M. Synthesis and in-vitro antibacterial activity of some new urea, thiourea and thiosemicarbazide derivatives. Int. J. Pharm. Pharm. Sci. 2012;4:379-83.
12.    Sarav A. Desai , Prakash S. Sukhramani, Maulik P. Suthar, Vipul P. Patel. Biological Cytotoxicity Evaluation of Sulfonamide Derivatives as Anti-Lung and Anti-Breast Cancer Activity. Asian J. Research Chem. 4(4): April, 2011; Page 671-677.
13.    Deepak Kumar Basedia, Birendra Shrivastava, B. K. Dubey, Pankaj Sharma. Synthesis and Anticancer Activity of Novel Substituted 1,3,4-oxadiazolo-[3,2-a]-1,3,5-triazine and 1,3,4-thiadiazolo-[3,2-a]-1,3,5-triazine Derivatives. Asian J. Research Chem. 7(3): March 2014; Page 310-315.
14.    Miller NJ, Rice-Evans CA. Factors influencing the antioxidant activity determined by the ABTS radical cation assay. Free Radical Research. 1997 Jan 1;26(3):195-9.
15.    Dawson KS, Schlegel DJ, Ahn CP, Anderson SF, Aubourg É, Bailey S, Barkhouser RH, Bautista JE, Beifiori A, Berlind AA, Bhardwaj V. The Baryon oscillation spectroscopic survey of SDSS-III. The Astronomical Journal. 2012 Dec 6;145(1):10.
16.    Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Research. 1987 Feb 15;47(4):936-42.
17.    Gerlier D, Thomasset N. Use of MTT colorimetric assay to measure cell activation. Journal of Immunological Methods. 1986 Nov 20;94(1-2):57-63.

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