Author(s): Panji Sananta, Sri Andarini, Respati Suryanto Dradjat, Umi Kalsum, Endang Sri Wahyuni, Elfiah, Ray Asaf Hexa Pandiangan, Lasa Dhakka Siahaan

Email(s): panjisananta@ub.ac.id

DOI: 10.52711/0974-360X.2022.00464   

Address: Panji Sananta1*, Sri Andarini2, Respati Suryanto Dradjat1, Umi Kalsum3, Endang Sri Wahyuni4, Elfiah5, Ray Asaf Hexa Pandiangan6, Lasa Dhakka Siahaan6
1Department of Orthopaedic and Traumatology, Pediatric Division, Saiful Anwar Hospital.
2Department of Public Health, Faculty of Medicine, Brawijaya University.
3Department of Pharmacology, Faculty of Medicine, Brawijaya University.
4Department of Physiology, Faculty of Medicine, Brawijaya University.
5Resident of Department of Orthopaedic and Traumatology, Saiful Anwar General Hospital.
6Department of Orthopaedic and Traumatology, Saiful Anwar General Hospital.
*Corresponding Author

Published In:   Volume - 15,      Issue - 6,     Year - 2022


ABSTRACT:
Introduction: Soft tissue engineering strategies targeting volume loss restoration has been developed as the current management of soft tissue defects. Important components of this method are cell sources, scaffold, and bioreactor. Adipose-derived mesenchymal stem cell (ADMSC) from a stromal vascular fraction (SVF) attached to scaffold and differentiated into defect tissue through cell communication. This study was aimed to investigate the viability of ADMSC from SVF combined with collagen, calcium alginate, oxidized cellulose, gelatin, and amnion membrane in vitro. Method: This true experimental done in the laboratory using 24 samples of SVF ADMSC that had been cryopreserved for 5 months and already get through the thawing process. Result: Based on the descriptive analysis with 4 repetition each group and control, gelatin shows average 90.928 and deviation standard 1,053, oxidized cellulose average 19,528 and deviation standard 2,543, collagen average 93,273 and deviation standard 1,195, calcium alginate average 92,953 and deviation standard 1,257, amnion membrane average 92,068 and deviation standard 2,467, control 95,995 and deviation standard 0,428. Conclusion: The use of gelatin, collagen, calcium alginate, and amnion membrane as scaffold show high viability results of ADMSC from SVF. Otherwise, oxidized cellulose scaffold has the lowest amount of viable cell. However, the most recommended scaffold material is amnion membrane because of the easiness of the production process, high availability, high potency of cell growth, and low cost.


Cite this article:
Panji Sananta, Sri Andarini, Respati Suryanto Dradjat, Umi Kalsum, Endang Sri Wahyuni, Elfiah, Ray Asaf Hexa Pandiangan, Lasa Dhakka Siahaan. Viability of Mesenchymal Stem Cells from Stromal Vascular Fraction to Tissue Engineering Scaffold Collagen, Calcium Alginate, Oxidized Cellulose, Gelatin, And Amnion Membrane (Primary Cell Culture in Vitro Study). Research Journal of Pharmacy and Technology. 2022; 15(6):2771-4. doi: 10.52711/0974-360X.2022.00464

Cite(Electronic):
Panji Sananta, Sri Andarini, Respati Suryanto Dradjat, Umi Kalsum, Endang Sri Wahyuni, Elfiah, Ray Asaf Hexa Pandiangan, Lasa Dhakka Siahaan. Viability of Mesenchymal Stem Cells from Stromal Vascular Fraction to Tissue Engineering Scaffold Collagen, Calcium Alginate, Oxidized Cellulose, Gelatin, And Amnion Membrane (Primary Cell Culture in Vitro Study). Research Journal of Pharmacy and Technology. 2022; 15(6):2771-4. doi: 10.52711/0974-360X.2022.00464   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-6-68


REFERENCES:
1.    Woo SL, Buckwalter JA. Injury and repair of the musculoskeletal soft tissues. J Orthop Res. 1988; 6(6):907-931. doi:10.1002/jor.1100060615
2.    Büchler U. Traumatic soft-tissue defects of the extremities. Implications and treatment guidelines. Arch Orthop Trauma Surg. 1990; 109(6):321-329. doi:10.1007/BF00636170
3.    Yuksel E, Choo J, Wettergreen M, Liebschner M. Challenges in Soft Tissue Engineering. Semin Plast Surg. 2005; 19(3):261-270. doi:10.1055/s-2005-919721
4.    Rantam, Fedik and Nugraha, Alexander and Mahyudin, Ferdiansyah and Purwati, Purwati and Bumi, Candra and Susilowati, Helen and Hendrianto, Eryk and Utomo, Dwikora and Suroto, Heri and Sumartono, Christrijogo and Setiawati, Rosy and Prakoeswa, Cita and Indramaya, Diah. A Potential Differentiation of Adipose and Hair Follicle-derived Mesenchymal Stem Cells to Generate Neurons Induced with EGF, FGF, PDGF and Forskolin. Research Journal of Pharmacy and Technology. 2020; (13)275. doi : 10.5958/0974-360X.2020.00056.6.
5.    Nugraha AP, Rezkita F, Puspitaningrum MS, Luthfimaidah MS, Narmada IB, Prahasanti C, et al. Gingival Mesenchymal Stem Cells and Chitosan Scaffold to Accelerate Alveolar Bone Remodelling in Periodontitis: A Narrative Review. Research J. Pharm. and Tech. 2020. 13(5). doi: 10.5958/0974-360X.2020.00446.1
6.    Nugraha AP, Ihsan IS, Dinaryant A, Hendrianto E, Susilowati H, Prasetyo EP, et al. Cobalt (II) Chloride in Enhancing Hypoxia Inducible Factor-1α Expression of Gingival Derived Mesenchymal Stem Cells in Vitro. Research J. Pharm. and Tech. 2021; 14(5):2639-2642. doi: 10.52711/0974-360X.2021.00465
7.    Salah RS, Ahmed HH, Abd-Allah SH, Hassan RE, Khalil WKB, Abd-Rabou AA, et al. The Anti-epileptic Efficiency of Mesenchymal Stem Cells Against Pilocarpine Model of Acute Epilepsy. Research J. Pharm. and Tech 2021; 14(3):1255-1266. doi: 10.5958/0974-360X.2021.00223.7
8.     Athanerey A, Verma NR, Bhargava P, Patra PK, Kumar A. Mesenchymal stem cells prove a significant role in Chronic non-healing ulcer progressive healing. Research J. Pharm. and Tech. 2021; 14(1):373-377. doi: 10.5958/0974-360X.2021.00068.8
9.     Suciadi SP, Nugraha AP, Ernawati DS, Ayuningtyas NF, Narmada IB, Prahasanti C, et al. The Efficacy of Human Dental Pulp Stem Cells in regenerating Submandibular Gland Defects in Diabetic Wistar Rats (Rattus novergicus). Research J. Pharm. and Tech. 2019; 12(4):1573-1579. doi: 10.5958/0974-360X.2019.00261.0
10.    Tiwari RK, Sharma V, Pandey R, Shukla SS. Stem Cells: Basics and its Prospective uses in Medical field. Research J. Pharm. and Tech. 2018.11(4). doi: 10.5958/0974-360X.2018.00285.8
11.    Indumathi S, Mishra R, Harikrishnan R, DHanasekaran M. Subcutaneous Adipose Tissue-Derived Stem Cell : Advancement and Applications in Regenerative Medicine. Compr Med Chem III. 2017; 1–8:379–435. doi: 10.1159/000448180
12.    Hanson SE, Gutowski KA, Hematti P. Clinical Application of Mesenchymal Stem Cells in Soft Tissue Augmentation. Aesthetic Surg J. 2014; 30(6):838–42. doi: 10.1177/1090820X10386364
13.    Bourin P, Bunnell BA, Casteilla L, et al. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy. 2013; 15(6):641-648. doi:10.1016/j.jcyt.2013.02.006
14.    Cheng, Xingguo. Biomaterials for Tendon/Ligament and Skin Regeneration. Biomaterials in Regenerative Medicine. 2018; (11)263-275. doi:10.5772/intechopen.69716
15.    Agarwal S, Jhunjhunwala V, Priya G. Fabrication and Morphological Analysis of Gelatin-Alginate Scaffolds. Research J. Pharm. and Tech 2018; 11(9): 3816-3818. doi: 10.5958/0974-360X.2018.00699.6
16.    Thein-Han WW, Saikhun J, Pholpramoo C, Misra RD, Kitiyanant Y. Chitosan-gelatin scaffolds for tissue engineering: physico-chemical properties and biological response of buffalo embryonic stem cells and transfectant of GFP-buffalo embryonic stem cells. Acta Biomater. 2009; 5(9):3453-3466. doi:10.1016/j.actbio.2009.05.012
17.    Hoque ME, Nuge T, Yeow TK, Nordin N, Prasad RGS V. Gelatin Based Scaffolds for Tissue Engineering – a Review. Polym Res J. 2015; 9(1):1935–2530.
18.    Vanstraelen P. Comparison of calcium sodium alginate (KALTOSTAT) and porcine xenograft (E-Z DERM) in the healing of split-thickness skin graft donor sites. Burns. 1992; 18(2):145-148. doi:10.1016/0305-4179(92)90014-l
19.    Sarode SM, Sagar GV, Kale MK, Nimase PK, Kulkarni AP, Firke SD. Preparation and Evaluation of Floating Calcium Alginate Beads of Clarithromycin. Research Journal of Pharmaceutical Dosage Forms and Technology. 2010.2(2):173-177
20.    Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Seifalian AM. Properties of the amniotic membrane for potential use in tissue engineering. Eur Cell Mater. 2008; 15:88-99. Published 2008 Apr 29. doi:10.22203/ecm.v015a07
21.    Devi LD. Knowledge and Practice about Placental Stem Cell and its utilization among various Health Professionals in selected hospitals, Bangalore, Karnataka. Int. J. of Advances in Nur. Management. 2019; 7(4): 324-330. doi: 10.5958/2454-2652.2019.00076.3
22.    Tommila M, Jokilammi A, Penttinen R, Ekholm E. Cellulose - A Biomaterial with Cell-Guiding Property. Cellul - Medical, Pharm Electron Appl. 2013. doi: 10.5772/54436
23.    Lin SD, Huang SH, Lin YN, et al. Engineering adipose tissue from uncultured human adipose stromal vascular fraction on collagen matrix and gelatin sponge scaffolds. Tissue Eng Part A. 2011; 17(11-12):1489-1498. doi:10.1089/ten.TEA.2010.0688

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available