Author(s): Mahmoud A. Abdel-Monem, Ahmed M. Salem, Karam A. Mahdy, Gamila S. M. El-Saeed, Abdel-Razik H. Farrag, Nahla S. Hassan

Email(s): ma.abdel-monem@nrc.sci.eg , mahmoud199018@gmail.com

DOI: 10.52711/0974-360X.2022.00460   

Address: Mahmoud A. Abdel-Monem1*, Ahmed M. Salem2, Karam A. Mahdy1, Gamila S. M. El-Saeed1, Abdel-Razik H. Farrag3, Nahla S. Hassan2
1Department of Medical Biochemistry, National Research Centre, Cairo, Egypt. Postal Code: 12622.
2Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
3Department of Pathology, National Research Centre, Cairo, Egypt.
*Corresponding Author

Published In:   Volume - 15,      Issue - 6,     Year - 2022


ABSTRACT:
Introduction: Hepatocyte nuclear factors HNF4a and HNF1a, key transcription factors that regulate drug metabolism enzymes expression, were linked to inflammation. Galectin-3 (Gal-3) is a multi-role animal lectin which is involved in inflammation. This study aims to evaluate the impact of galectin-3 inhibition, by using modified citrus pectin (MCP), on HNF4a and HNF1a gene expression levels after acetaminophen (APAP) induced acute liver injury in Wistar rats. Materials and Methods: Sixty-four male Wistar rats were divided into four groups as follows; control, MCP, APAP and MCP plus APAP administered groups. The groups received APAP were divided into three subgroups each; in which rats were sacrificed after 24, 48 and 72 hours (h) from APAP administration. Expression levels of HNF4a and HNF1a, beside levels of Gal-3, tumor necrosis factor- a (TNF-a), Cytochrome P450 2E1 (CYP2E1), reduced glutathione (GSH), glutathione reductase (GR) and peroxidase (GPx) activities, liver function parameters were evaluated, along with histopathological study of the liver. Results: APAP high dose induced inhibition of liver HNF4a and HNF1a gene expression, CYP2E1 and GSH levels, GR and GPx activities, and increased hepatic Gal-3, TNF-a and serum liver function parameters levels, besides inducing hepatic necrosis. The toxic effects were stronger after 24 h then declined gradually after 48 h and 72 h. Inhibiting Gal-3 functionality after APAP high dose administration reduced TNF-a level and retrieved liver levels of HNF4a and HNF1a expression, CYP2E1, GSH, GR and GPx closer to normal control levels. Conclusion: Inhibiting Gal-3 functionality affects HNF4a and HNF1a gene expression levels and reduced inflammation after APAP high dose administration.


Cite this article:
Mahmoud A. Abdel-Monem, Ahmed M. Salem, Karam A. Mahdy, Gamila S. M. El-Saeed, Abdel-Razik H. Farrag, Nahla S. Hassan. Galectin-3 inhibition retained expression of hepatocyte nuclear factors 4α and 1α in acetaminophen induced acute liver injury. Research Journal of Pharmacy and Technology. 2022; 15(6):2747-5. doi: 10.52711/0974-360X.2022.00460

Cite(Electronic):
Mahmoud A. Abdel-Monem, Ahmed M. Salem, Karam A. Mahdy, Gamila S. M. El-Saeed, Abdel-Razik H. Farrag, Nahla S. Hassan. Galectin-3 inhibition retained expression of hepatocyte nuclear factors 4α and 1α in acetaminophen induced acute liver injury. Research Journal of Pharmacy and Technology. 2022; 15(6):2747-5. doi: 10.52711/0974-360X.2022.00460   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-6-64


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