A. Najitha Banu, Anand Singh, A.M. Raut, Johnson Wahengbam
A. Najitha Banu1*, Anand Singh1, A.M. Raut2, Johnson Wahengbam2
1Department of Zoology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.
2Department of Entomology, Szent Istvan University.Budapest, Hungary.
Volume - 15,
Issue - 6,
Year - 2022
Nanotechnology has proved a boon as it brings a revolutionary change in the world with the tremendous advancements in the field of science such as engineering, biotechnology, analytical chemistry, and agriculture. The synthesis of the metal nanoparticle is a developing area of exploration in present-day material science and innovation. Among the techniques involved in the synthesis of metallic nanoparticles, the biological methods or the green synthesis are reliable due to its cost-effective, environment friendly, and use of non-harmful materials in the processing. Therefore, the current work is focused on the biosynthesis of silver nanoparticles with the leaf extract of holy plant Aegle marmelos by utilizing 1mM silver nitrate solution. From the bio-reduction reaction mixture, the synthesis of silver nanoparticles was studied by UV-Visible spectrophotometer at the range of 200 nm to 800nm and discovered maximum absorbance at 460nm. Further, the SNPs were analyzed for the protein or other organic groups by Fourier Transformed Infrared spectrophotometer (FTIR). The dried silver nanoparticles were further characterized by utilizing a scanning electron microscope (SEM) to observe the actual size, shape, and distribution of particles. This result showed the actual size of the bioengineered silver nanoparticles ranges from 25-30nm. The EDX result showed a peak of Ag that confirmed its presence in the suspension. The main objective of this study is to develop a fast, environmentally friendly, and advantageous technique for the synthesis of silver nanoparticles. By standardizing the biosynthesis techniques, silver nanoparticles can be synthesized in bulk with a safe environment and it may be utilized for different clinical applications including control of pathogenic microorganisms and so on.
Cite this article:
A. Najitha Banu, Anand Singh, A.M. Raut, Johnson Wahengbam. Phytofabrication of Silver Nanoparticles using aqueous leaf extract of Aegle marmelos and its Characterization. Research Journal of Pharmacy and Technology. 2022; 15(6):2709-4. doi: 10.52711/0974-360X.2022.00453
A. Najitha Banu, Anand Singh, A.M. Raut, Johnson Wahengbam. Phytofabrication of Silver Nanoparticles using aqueous leaf extract of Aegle marmelos and its Characterization. Research Journal of Pharmacy and Technology. 2022; 15(6):2709-4. doi: 10.52711/0974-360X.2022.00453 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-6-57
1. Salata O V. Applications of nanoparticles in biology and medicine. Journal of Nanobiotechnology. 2004; 2(1), 3.
2. Ong C. Yung, LYL. Cai Y. Bay BH. And Baeg G H. Drosophila melanogaster as a model organism to study nanotoxicity. Nanotoxicology, 2015;9(3), 396-403.
3. Bhatia S. Nanoparticles types, classification, characterization, fabrication methods and drug delivery applications. In Natural Polymer Drug Delivery Systems. 2016; 33-93.
4. Bahadar H. Maqbool F. Niaz K.and Abdollahi M. Toxicity of nanoparticles and an overview of current experimental models. Iranian Biomedical Journal, 2016; 20(1), 1.
5. Albrecht MA. Evans CW. and Raston CL. Green chemistry and the health implications of nanoparticles. Green Chemistry, 2006; 8(5), 417-432.
6. Goodsell DS. Bionanotechnology: lessons from nature. John Wiley and Sons. 2004.
7. Abdel-Halim ES. El-Rafie M.H. and Al-Deyab S.S. Polyacrylamide/guar gum graft copolymer for preparation of silver nanoparticles. Carbohydrate Polymers, 2011; 85(3), 692-697.
8. Brahmachari UN, The role of science in recent progress of medicine, Current Science 2001; 81: 15- 16.
9. Dandapat S. Kumar M. Kumar A. and Sinha MP. Antipathogenic Efficacy of Methanolic leaf extract of Cinnamomum tamala (Buch.-Ham.) and Aegle marmelos (L.) with their nutritional potentiality. The Bioscan, 2013;.8(2), 635-641.
10. Bandow J E. Brötz H. Leichert LIO. Labischinski H.and Hecker M. Proteomic approach to understanding antibiotic action. Antimicrobial agents and chemotherapy, 2003; 47(3), 948-955.
11. Atmakuri LR. and Dathi S. Current trends in herbal medicines. Journal of Pharmaceutical Research, 2010; 3(1), 109-113.
12. Dhankhar S.. Ruhil S. Balhara M. Dhankhar S. and Chhillar AK, Journal of Medicinal Plants Research, 2011; 5, 1497-1507.
13. Najitha Banu A. Raut AM and Balasubramanian C. Bioengineered nanoparticles synthesized using Ipomoea pes-tigridis for improved antimicrobial activity against drug resistant microbes. International Journal of Zoology and Applied Biosciences, 2017; 2(6): 338-347.
14. Patil R H. Bhushan C. and Sailaxmi S. Antifungal and Antiaflatoxigenic activity of Aegle marmelos Linn. Pharmacognosy Journal, 2009; 1(4).
15. Nithya Deva Krupa A. and Raghavan V. Biosynthesis of silver nanoparticles using Aegle marmelos (Bael) fruit extract and its application to prevent adhesion of bacteria: a strategy to control microfouling. Bioinorganic Chemistry and Applications, 2014.
16. Devaraj Sabapathy PC. Parthiban A. Pugazhendhi A. and Kathirvel P. Aegle marmelos: a novel low cost substrate for the synthesis of polyhydroxyalkanoate by Bacillus aerophilus RSL-7. Biocatalysis and Agricultural Biotechnology, 2019; 18, 101021.
17. Patil SV. Borase HP. Patil CD. and Salunke B K. Biosynthesis of silver nanoparticles using latex from few euphorbian plants and their antimicrobial potential. Applied Biochemistry and Biotechnology, 2012; 167(4), 776-790.