Author(s): Layla O. Farhan, Ahlam M. Farhan, Sundus Al Obaidi, Ekhlass M. Taha

Email(s): laylaof_chem@csw.uobaghdad.edu.iq

DOI: 10.52711/0974-360X.2022.00444   

Address: Layla O. Farhan1*, Ahlam M. Farhan1, Sundus Al Obaidi2, Ekhlass M. Taha1
1Department of Chemistry, College of Science for Women, University of Baghdad, Al-Jadriya Baghdad Iraq.
2Baghdad Teaching Hospital, DXA Unit, Baghdad, Iraq.
*Corresponding Author

Published In:   Volume - 15,      Issue - 6,     Year - 2022


ABSTRACT:
The current study aimed to ascertain the levels of matrix metalloproteinase-12 (MMP-12) and Lysyl oxidase (LOX) in osteoporosis patients and their correlation with alkaline phosphatase (ALP), magnesium (Mg), vitamin D (Vit D), calcium (Ca), phosphorus (P), and T-score %. 110 participants recruited from Baghdad Teaching Hospital, Iraq, were enrolled in this study from November 2019 to March 2020). The participants were divided into two groups: Group 1 comprised 60 osteoporotic women and group 2 consisted of 50 healthy women. (MMP and LOX) were estimated using a quantitative enzyme-linked immunosorbent assay (ELISA. The results showed significant differences in serum LOX, age, ALP, Mg, and T-score %, while no significant differences in serum MMP-12, Vit D, Ca, P, and body mass index (BMI) were noticed in osteoporosis patients as compared to the control group. The correlation analysis showed a significant correlation of T-score with Vit D and MMP12 in osteoporosis patients, when a t-test was conducted with the two participant groups. Moreover, no significant correlation was found between the T-score result and LOX, Mg, Ca, and P, which indicated that the relation was not linear because the change in the variables neither increased nor decreased systematically compared to the T-score. LOX, ALP, and Mg were significantly associated with osteoporosis patients, while MMP12 and Vit D were found to affect the density of the bone. Therefore, the studied parameters may be used in the form of a bone mineral density decline index.


Cite this article:
Layla O. Farhan, Ahlam M. Farhan, Sundus Al Obaidi, Ekhlass M. Taha. A Case-control Study to Determine Metalloproteinase-12 and Lysyl Oxidase Levels in Iraqi women with Osteoporosis. Research Journal of Pharmacy and Technology. 2022; 15(6):2655-0. doi: 10.52711/0974-360X.2022.00444

Cite(Electronic):
Layla O. Farhan, Ahlam M. Farhan, Sundus Al Obaidi, Ekhlass M. Taha. A Case-control Study to Determine Metalloproteinase-12 and Lysyl Oxidase Levels in Iraqi women with Osteoporosis. Research Journal of Pharmacy and Technology. 2022; 15(6):2655-0. doi: 10.52711/0974-360X.2022.00444   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-6-48


REFERENCES:
1.    Chitra V. Ali M. Animal Models for Osteoporosis-A Review. Research Journal of Pharmacy and Technology. 2020; 13(3):1543–1548. doi: 10.5958/0974-360X.2020.00280.2
2.    Sözen T. Özışık L. Başaran N. An Overview And Management of Osteoporosis. European Journal of Rheumatology. 2017; 4(1):46. doi: 10.5152/eurjrheum.2016.048
3.    Padmanabhan K. Sudhakar S. Selvam P. Kirthika S. Veena. Which is More Prevalent Among the Female Population-Osteopenia or Osteoporosis? A cross sectional study. Research Journal of Pharmacy and Technology. 2019; 12(3):1163–1168. DOI: 10.5958/0974-360X.2019.00192.6
4.    Khoshnood Z. Anoosheh M. Zadej EH. A Description of Osteoporosis Preventive Behaviors in Iranian Adolescent Girls. Asian Journal of Nursing Education and Research. 2016; 6(1):1–4. DOI : 10.5958/2349-2996.2016.00001.X
5.    Strukov V. Kislov A. Eremina N. Deriabina G. Sergeeva M. Antropov A. Kuzmina Y.et al. The use of Bone Tissue Non-Steroid Anabolizators in Treatment of Osteoporosis. Research Journal of Pharmacy and Technology. 2019; 12(5):2195–2199. DOI: 10.5958/0974-360X.2019.00366.4
6.    Blackie R. Diagnosis, assessment and management of osteoporosis. Prescriber. 2020; 31(1):14–19.
7.    Cosman F.de Beur SJ. LeBoff MS. Tanner B. Randall S. Lindsay R. Clinician’s guide to prevention and treatment of osteoporosis. Osteoporosis international. 2014; 25(10):2359–2381.
8.    Kumar T. Dhanapal C. Sudhakar M. Bhanja S. Akhila A. Prakash P. Significance of a chilles Ultrasonometer in the precised Diagnosis and Assessment of Osteoporosis. Research Journal of Pharmacy and Technology. 2020; 13(3):1592–1596. DOI : 10.5958/0974-360X.2020.00288.7
9.    Ismael MK. The Prognostic Value of some Epithelial-Mesenchymal Transition Markers and Metastasis-Related Markers in Human Transitional Cell Carcinoma of the Bladder. Baghdad Science Journal. 2018; 15(3). DOI: http://dx.doi.org/10.21123/bsj.2018.15.3.0244
10.    Lu P. Takai K. Weaver V. Zena W. Extracellular matrix degradation and remodeling in development and disease. Cold Spring Harbor perspectives in biology. 2011; 3(12): doi: 10.1101/cshperspect.a005058
11.    Mittal R. Patel A. Debs L. Desiree N. Kunal P. M'hamed G. Jeenu M. Denise Y. et al. Intricate functions of matrix metalloproteinases in physiological and pathological conditions. Journal of cellular physiology. 2016; 231(12):2599 2621.doi.org/10.1002/jcp.25430
12.    Ren Z-H. Wu K. Yang R. Zhe-Q. Wei C. Differential expression of matrix metalloproteinases and miRNAs in the metastasis of oral squamous cell carcinoma. BMC Oral Health. 2020; 20(1): doi.org/10.1186/s12903-020-1013-0
13.    Tokuhara C. Santesso M. Oliveira G. Talita M. Julio T. Willian F. Rodrigo C. Updating the role of matrix metalloproteinases in mineralized tissue and related diseases. Journal of Applied Oral Science. 2019; 27. doi.org/10.1590/1678-7757-2018-0596
14.    Jangde R. Matrix metalloproteinase: An overview. Research Journal of Science and Technology. 2011; 3(6):301–307. DOI: 10.5958/0974-360X.2020.00493.X
15.    Stone MJ. Chowdrey P. Miall P. Validation of an enzymatic total magnesium determination based on activation of modified isocitrate dehydrogenase. Clinical chemistry. 1996;42(9):1474–1477. doi.org/10.1093/clinchem/42.9.1474
16.    Vijayakumar V. Radhakrishnan N. Vasantha P. Molecular Docking Analysis of Triazole Analogues as Inhibitors of Human Neutrophil Elastase (HNE), Matrix Metalloproteinase (MMP 2 and MMP 9) and Tyrosinase. Research Journal of Pharmacy and Technology. 2020; 13(6):2777–2783. DOI: 10.5958/0974-360X.2020.00493.X
17.    Wolf M. Clay S. Zheng S. Pan P. Chan M. MMP12 Inhibits Corneal Neovascularization and Inflammation through Regulation of CCL2. Scientific reports. 2019; 9(1):1–13.  doi.org/10.1038/s41598-019-47831-z
18.    Hao W. Zhang Y. Yunqing Z. Cailian Z. Yani X. Expressions of MMP-12, TIMP-4, and neutrophil elastase in PBMCs and exhaled breath condensate in patients with COPD and their relationships with disease severity and acute exacerbations. Journal of Immunology Research. 2019;2019. doi.org/10.1155/2019/7142438
19.    Vallet SD. Ricard-Blum S. Lysyl oxidases: from enzyme activity to extracellular matrix cross-links. Essays in Biochemistry. 2019;63(3):349–364.doi10.1042/EBC2018005
20.    Grimsby L. Lucero H. Trackman P. Herbert M. Role of lysyl oxidase propeptide in secretion and enzyme activity. Journal of Cellular Biochemistry. 2010;111(5):1231–1243. https://doi.org/10.1002/jcb.22845
21.    Vallet SD. Guéroult M. Belloy N. Manuel D. Sylvie R. A three-dimensional model of human lysyl oxidase, a cross-linking enzyme. American Chemical Society. 2019;4(5):8495–8505. doi.org/10.1021/acsomega.9b00317
22.    Li T. Wu C. Gao L. Feng Q. Qiang W. Jiuhong Y. Lysyl oxidase family members in urological tumorigenesis and fibrosis. Oncotarget. 2018; 9(28):20156.  doi: 10.18632/oncotarget.24948
23.    Afratis N. Klepfish M. Karamanos N. Irit S. The apparent competitive action of ECM proteases and cross-linking enzymes during fibrosis: applications to drug discovery. Advanced Drug Delivery Reviews. 2018; 129:4–15.  doi.org/10.1016/j.addr.2018.03.004
24.    Laczko R. Csiszar K. Lysyl Oxidase (LOX): Functional Contributions to Signaling Pathways. Biomolecules. 2020; 10(8):1093. doi.org/10.3390/biom10081093
25.    Lopes HEG. Gosling M de S. Cluster analysis in practice: Dealing with outliers in managerial research. Revista de Administração Contemporânea. 2021; 25(1). https://doi.org/10.1590/1982-7849rac2021200081
26.    Hamadi HS. Hamza MI. Malik AS. Evaluation of Serum Lysyl oxidase and Calreticulin as Biomarkers in patients with Chronic Kidney Disease. Indian Journal of Public Health Research and Development. 2019; 10(10):1730–1735.
27.    Cai L. Xiong X. Kong X. Jing X. The role of the lysyl oxidases in tissue repair and remodeling: a concise review. Tissue Engineering and Regenerative Medicine. 2017; 14(1):15–30.doi.org/10.1007/s13770-016-0007-0
28.    Xie J. Jiang J. Huang W. Yanjun Z. Chunming X. Chunli W. LinY.et al. TNF-α induced down-regulation of lysyl oxidase family in anterior cruciate ligament and medial collateral ligament fibroblasts. The Knee. 2014; 21(1):47–53. doi.org/10.1016/j.knee.2012.12.015
29.    Kumari S.Panda TK. Pradhan T. Lysyl oxidase: its diversity in health and diseases. Indian Journal of Clinical Biochemistry. 2017; 32(2):134–141. doi.org/10.1007/s12291-016-0576-7
30.    Liang H. Xu J. Xue M. Christopher J. Matrix metalloproteinases in bone development and pathology: current knowledge and potential clinical utility. Metalloproteinases Med. 2016; 3:93–102. doi.org/10.2147/MNM.S92187
31.    He M. Le Y. Zhang Y. Ouyang H. Jian P. Shan Z. Juan L. et al. Matrix metalloproteinase 12 expression is associated with tumor FOXP3+ regulatory T cell infiltration and poor prognosis in hepatocellular carcinoma. Oncology Letters. 2018; 16(1):475–482. doi.org/10.3892/ol.2018.8642
32.    Kwon S. Weiden MD. Echevarria GC. Comfort A. Naveed B. Prezant W. William N. et al. Early elevation of serum MMP-3 and MMP-12 predicts protection from World Trade Center-lung injury in New York City Firefighters: a nested case-control study. PloS One. 2013; 8(10): 1-8. doi.org/10.1371/journal.pone.0076099

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available