Author(s): Shafira Kurnia, Chiquita Prahasanti, Onge Victoria Hendro, Yokthan Ofier Siki, Wibi Riawan, Lambang Bargowo

Email(s): chiquita-p-s@fkg.unair.ac.id

DOI: 10.52711/0974-360X.2022.00442   

Address: Shafira Kurnia1, Chiquita Prahasanti1*, Onge Victoria Hendro2, Yokthan Ofier Siki2, Wibi Riawan3, Lambang Bargowo1
1Lecturer, Department of Periodontology, Faculty of Dental Medicine, Airlangga University, Surabaya – Indonesia.
2Resident, Graduate Program in Periodontology, Faculty of Dental Medicine, Airlangga University, Surabaya – Indonesia.
3Department of Biomolecular Biochemistry, Faculty of Medicine, Brawijaya University, Malang – Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 6,     Year - 2022


ABSTRACT:
Background and Aim: The current goal of periodontal therapy is to achieve periodontal regeneration. Important factor for periodontal regeneration is to promote bone formation, nowadays used bone replacement. The used of xenograft materials as gold standard for periodontal tissue regeneration using type I collagen bone graft has been widely developed. The main organic component in fish scales is type I fibril collagen, which are same as with component in bones. Specific markers of bone formation are the expression of osteoblast, osteoclast, osteoprotegerin (OPG), and receptor activator of nuclear factor ?B ligands (RANKL). The purpose of this study is to assess the expression of OPG and RANKL after application of extract collagen from gourami (Osphronemus gouramy) scales. Materials and Methods: Thirty-two experimental 3-month-old male Wistar albino rats (150g and 200g) were randomly divided into four groups: 7-day control group, 7-day fish collagen group, 14-day control group and 14-day fish collagen group. The sockets were filled with blood (control group), whereas 10 mg to 30 mg extract collagen was added until the sockets were fully occupied (treatment group). Results: The ANOVA test showed a significance level of 0.000 (p <0.05). Conclusion: Expression of OPG enhanced and expression of RANKL lowered after application of type I collagen from gourami scales, accelerating osteogenesis.


Cite this article:
Shafira Kurnia, Chiquita Prahasanti, Onge Victoria Hendro, Yokthan Ofier Siki, Wibi Riawan, Lambang Bargowo. OPG and RANKL Signal Transduction in Osteoblast Regulation Post Application Extract Collagen in Osteogenesis. Research Journal of Pharmacy and Technology. 2022; 15(6):2645-9. doi: 10.52711/0974-360X.2022.00442

Cite(Electronic):
Shafira Kurnia, Chiquita Prahasanti, Onge Victoria Hendro, Yokthan Ofier Siki, Wibi Riawan, Lambang Bargowo. OPG and RANKL Signal Transduction in Osteoblast Regulation Post Application Extract Collagen in Osteogenesis. Research Journal of Pharmacy and Technology. 2022; 15(6):2645-9. doi: 10.52711/0974-360X.2022.00442   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-6-46


REFERENCES:
1.     Ajay M, Negi KS, Saroj T, Kanwarjeet AS. A successfully treated case of severe periodontitis using interdisciplinary approach: Report of a case.  Journal of Indian Society of Periodontology. 2016; 20(1):95-97. https://doi.org/10.4103/0972-124X.168496.
2.     Keestra JAJ, Barry O, Jong LD, Wahl G. Long-term effects of vertical bone augmentation : a systematic review. J Appl Oral Sci. 2016; 24(1): 3–17. http://dx.doi.org/10.1590/1678-775720150357.
3.     Kandwal A, Bhardwaj J, Sunny, Batra M. Bone Grafts In Periodontal Surgery . A Review. Journal of Dental Herald. 2014; 1(3):30–32.
4.    Sakat BT, Sakhare RB, Suryvanshi UC, Kore PS, Mohite SK, Magdum CS. Osteoporosis: The Brittle Bone. Asian Journal Pharmaceutical Research.2018; 8(1):39-43. doi: 10.5958/2231-5691.2018.00008.4.
5.    Shailaja KS, Saraswati P. Collagen Vascular Disease-A Review. International Journal of Advances in Nursing Management 3(4): Oct.-Dec. 2015; Page 382-384. doi: 10.5958/2454-2652.2015.00040.2
6.    Shetty P, Chacko N, Alva A, Kumar V, Kandige PS, Gururaj MP, Joshi H, D’souza UP. Wound Healing Potential of Psidium guajava var, Pyrifera. Research Journal of Pharmacy and Technology. 2019; 12(12): 6067-6070. doi: 10.5958/0974-360X.2019.01053.9
7.    Ostrowski MC. A New Role for OPG: Putting RANKL in Its Place. Journal of Bone Mineral Research. 2010; 25(9):1905-6. doi: 10.1002/jbmr.206.
8.    Kalaivani C, Kuppusamy G, Saikamal, Karri VVSR. Simvastatin Loaded Polycaprolactone-collagen Scaffolds dor the treatment of Diabetic Foot Ulcer. . Research Journal of Pharmacy and Technology. 2019; 12(6): 2637-2644. doi: 10.5958/0974-360X.2019.00441.4.
9.    Yamada S, Yoshizawa Y, Kawakubo A, Ikeda T, Yanagiguchi K, Hayashi Y. Early gene and protein expression associated with osteoblast differentiation in response to fish collagen peptides powder. Dental Materials Journal. 2013; 32(2):233–40. https://doi.org/10.4012/dmj.2012-188
10.    Yamada S, Yamamoto K, Ikeda T, Yanagiguchi K, Hayashi Y. Potency of Fish Collagen as a Scaffold for Regenerative Medicine.  BioMed Research International. 2014; 2014(3):1–8. http://dx.doi.org/10.1155/2014/302932.
11.    Rachmasari A, Kartini RS, Alviani G, Solihah I. Healing effect of Spray gel collagen extract from Channa striata bone on burn in rats. Research Journal of Pharmaceutical Dosage Forms and Technology. 2019; 11(4):275-279. doi: 10.5958/0975-4377.2019.00046.6.
12.    Zulkifeli NRAN, Zain HHM, Zainol I, Musa NHC. The properties of Hydrolysed Collagen from Oreochromis mossambicus’s scale and their effect towards Cell viability. Research Journal of Pharmacy and Technology.2020; 13(12): 5855-5860. doi: 10.5958/0974-360x.2020.01020.3.
13.    Zahrani RA. Extraction and Isolation of Collagen Type I from Fish Skin. New Zealand: University of Otago, Dunedin; 2010. Available from: https://www.semanticscholar.org/paper/Extraction-and-Isolation-of-Collagen-Type-I-from-Zahrani-Chen/fc1e3e67fad3aa99fb1cbe8dc35c4a92ab3f62ea
14.    Nagai T, Izumi M, Ishii M. Fish scale collagen. Preparation and partial characterization. International Journal of Food Science and Technology. 2014;39(3): 239–244. doi: 10.1111/j.1365-2621.2004.00777.x.
15.    Oliviya CR. Production of ecofriendly silver nanoparticle and evaluation of its potential antimicrobial activity. Research Journal of Pharmacy and Technology. 2015; 8(10):1374-1378. doi: 10.5958/0974-360X.2015.00246.2.
16.    Prahasanti C, Wulandari DT, Ulfa N. Viability test of fish scale collagen (Oshpronemus gouramy) on baby hamster kidney fibroblasts-21 fibroblast cell culture. Veterinary World. 2018; 11(4):506–10.
17.    Ridhanya RK. Skin Wound Healing: An update on the Current knowledge and Concepts. Research Journal of Pharmacy and Technology. 2019; 12(3): 1448-1452. doi: 10.5958/0974-360X.2019.00240.3
18.    Arif R, Jaffat HS. Assessment of Procollagen 111N Terminal Peptide Level in Rats Undergo CCl4 and Bile Duct Ligation. Research Journal of Pharmacy and Technology. 2017; 10(7): 2132-2135. doi: 10.5958/0974-360X.2017.00374.2
19.    Yani S, Soeharto S, Sumarno, Kalim H. The Effect of Eleutherine Americana Merr. Extract on Expression Changes of MMP-8 and Type 1 Collagen in Periodontitis Rat Models. Research Journal of Pharmacy and Technology. 2020; 13(5): 2407-2412. doi: 10.5958/0974-360X.2020.00432.1
20.    Shi S, Kirk M, Kahn AJ. The Role of Type I Collagen in the Regulation of the Osteoblast Phenotype. Journal of Bone and Mineral Research. 2009; 11(8):1139–1145. https://doi.org/10.1002/jbmr.5650110813
21.    Kasagi S, Chen W. TGF-beta1 on osteoimmunology and the bone component cells. Cell and Bioscience. 2013; 3(4):1-7. doi: 10.1186/2045-3701-3-4
22.    Kim JH, Kim N. Signaling Pathways in Osteoclast Differentiation. Chonnam Medical Journal. 2016;52;12-17. http://dx.doi.org/10.4068/cmj.2016.52.1.12
23.    Neve A, Corrado A, Cantatore FP. Osteoblast physiology in normal and pathological conditions. Cell and Tissue Research. 2011;343(2): 289–302. doi: 10.1007/s00441-010-1086-1
24.    Newman MG, Takei HH, Klokkevold PR, Carranza FA. Carranza’s Clinical Periodontology. 12th ed. ELSEVIER: Saunder; 2015.
25.    Liu C, Sun J. Hydrolyzed Tilapia Fish Collagen Induces Osteogenic Differentiation of Human Periodontal Ligament Cell. Biomedical Materials. 2015; 10(6):1-9. doi:10.1088/1748-6041/10/6/065020
26.    Bareil RP, Gauvin R, Berthod F. Collagen-Based Biomaterials for Tissue Engineering Applications. Materials. 2010; 3:1863–1887. https://doi.org/10.3390/ma3031863
27.    Tang J, Saito T. Biocompatibility of Novel Type I Collagen Purified from Tilapia Fish Scale : An In Vitro Comparative Study. BioMed Research International. 2015; 2015:1–8. doi: 10.1155/2015/139476
28.    Capati MLF, Nakazono A, Yamamoto K, Sugimoto K, Yanagiguchi K, Yamada S, Hayashi Y. Fish Collagen Promotes the Expression of Genes Related to Osteoblastic Activity. International Journal of Polymer Science. 2016; 2016:1–8. http://dx.doi.org/10.1155/2016/5785819
29.    Teitelbaum SL, Amer YA, Ross FP. Molecular Mechanisms of Bone Resorption. J Cell Biochem. 1995; 59(1):1-10. doi: 10.1002/jcb.240590102
30.    Naghsh N, Razavi SM, Minaiyan M, Shahabooei M, Birang R, Behfarnia P, Hajisadeghi S. Evaluation of the effects of two different bone resorption inhibitors on osteoclast numbers and activity: An animal study. Dental Research Journal. 2016; 13(6):500–507. https://doi.org/10.4103/1735-3327.197034
31.    Delgado-Calle J, Sañudo C, Sumillera M, Garcés CM, Riancho JA. Expression of RANKL and OPG in primary osteoblasts. Rev Osteoporos Metab Miner. 2012; 4(4):133–138.
32.    Boyce BF, Li J, Xing L, Yao Z. Bone Remodeling and the Role of TRAF3 in Osteoclastic Bone Resorption.  Frontiers in Immunology. 2018;8:1-12. doi: 10.3389/fimmu.2018.02263
33.    Maxhimer JB, Bradley JP, Lee JC. Signaling pathways in osteogenesis and osteoclastogenesis: Lessons from cranial sutures and applications to regenerative medicine. Genes and Diseases. 2015; 2(1):57–68. https://doi.org/10.1016/j.gendis.2014.12.004
34.    Liu W, Zhang X. Receptor activator of nuclear factor   κ B ligand (RANKL)/ RANK / osteoprotegerin system in bone and other tissues (Review). Molecular Medicine Reports. 2015;11:3212-3218. https://doi.org/10.3892/mmr.2015.3152
35.    Khosla S. Minireview: The OPG/RANKL/RANK System. Endocrinology. 2001; 142(12):5050-5055. https://doi.org/10.1210/endo.142.12.8536

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available