Background: Olive oil is a natural source of phytoestrogens and antioxidants similar to mammalian estrogen. The substance increases osteoblast differentiation and osteoprotegerin (OPG) expression. Objective of the study: To examine the effect of giving olive oil on OPG expression of gingival crevicular fluid in the orthodontic tooth movement among old and young guinea pigs. Methodology: The four groups of guinea pigs (n = 3) consisted of old guinea pigs given olive oil (OO), old controls (OC), the young given olive oil (YO), and young controls (YC). The teeth were movedreciprocally to distal by a 0.35 N open coil spring which was attached to the bracket of the lower incisors. The YO group was given olive oil at 0.7ml/day dosage and the OO group had 1.86ml olive oil administered per day until day 14. OPG expression was analyzed in gingival crevicular fluid (GCF) sample using the enzyme-linked immunosorbent assay (ELISA) on days 0, 7, and 14. Retrieved data was analyzed using Three-way ANOVA Statistic Test followed by Post Hoc LSD Test. Results: The statistical test showed that olive oil increased OPG expression in old and young guinea pigs (p<0.05), and OPG OO expression was comparable to that in YC day 7. OPG YO expression was higher than OO (p <0.05). Increasing duration of olive oil application led to higher OPG expression (p <0.05). There was a correlation between olive oil and age and duration of administration (p <0.05). Conclusion: Olive oil increased the OPG expression on GCF of old and young guinea pigs during orthodontic tooth movement, with the highest OPG expression in YO day 14.
Cite this article:
Dina Listyowati, Sri Suparwitri, Cendrawasih Andusyana Farmasyanti. Effect of Olive oil on Osteoprotegerin Expression on the Tension side of Gingival Crevicular Fluid in the Orthodontic Tooth Movement of Old and Young Guinea Pigs. Research Journal of Pharmacy and Technology. 2022; 15(6):2621-6. doi: 10.52711/0974-360X.2022.00438
Dina Listyowati, Sri Suparwitri, Cendrawasih Andusyana Farmasyanti. Effect of Olive oil on Osteoprotegerin Expression on the Tension side of Gingival Crevicular Fluid in the Orthodontic Tooth Movement of Old and Young Guinea Pigs. Research Journal of Pharmacy and Technology. 2022; 15(6):2621-6. doi: 10.52711/0974-360X.2022.00438 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-6-42
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