Author(s): Suhad Yassein Abed, Ali Haider Alsakini, Khetam Habeeb Rasool, Sundus Qasim Mohammed, Sadeq Abdulridha Gatea Kaabi

Email(s): sadeqkaabi@uomustansiriyah.edu.iq

DOI: 10.52711/0974-360X.2022.00435   

Address: Suhad Yassein Abed, Ali Haider Alsakini, Khetam Habeeb Rasool, Sundus Qasim Mohammed, Sadeq Abdulridha Gatea Kaabi
Biology Department, College of Science, Mustansiriyah University, Baghdad – Iraq.
*Corresponding Author

Published In:   Volume - 15,      Issue - 6,     Year - 2022


ABSTRACT:
A number of 26 phages lytic for 26 isolates of V. cholerae O1, biotype El Tor, Serotype Inaba are isolated from sewage water. The phage isolates showed host range of 35-65% against V. cholerae O1 by spot lysis methods. The morphological properties of plaques formed on the top agarose are studied and showed that most virulent phages had larger diameter (in millimeter) with regular or irregular margin cut and clear plaque comparing with smaller diameter and turbid plaque for those less virulent phages. A total of 8 phages are selected for formulation the phage cocktail in order to extend the host-range of phages in cocktail collectively. The %inhibition assay for every single phage of the 8 phages selected for formulation of phage cocktail ranged from 0-75, whereas that of the 8 phages cocktail was 100%inhibition against all V. cholerae O1 isolates. The formulation of the 8 phages in a cocktail proved to an effective approach to achieve the broad host-range activity towards V. cholerae O1 isolates and made it possible to go forwards the animal model for further studies on phage therapy for animal model of cholera.


Cite this article:
Suhad Yassein Abed, Ali Haider Alsakini, Khetam Habeeb Rasool, Sundus Qasim Mohammed, Sadeq Abdulridha Gatea Kaabi. Formulation of a Novel Phage Cocktail against Vibrio cholerae O1. Research Journal of Pharmacy and Technology. 2022; 15(6):2605-8. doi: 10.52711/0974-360X.2022.00435

Cite(Electronic):
Suhad Yassein Abed, Ali Haider Alsakini, Khetam Habeeb Rasool, Sundus Qasim Mohammed, Sadeq Abdulridha Gatea Kaabi. Formulation of a Novel Phage Cocktail against Vibrio cholerae O1. Research Journal of Pharmacy and Technology. 2022; 15(6):2605-8. doi: 10.52711/0974-360X.2022.00435   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-6-39


REFERENCES:
1.    Ramamurthy T. Mutreja A. Weill F-X. Das B. Ghosh A. Nair GB. Revisiting the Global Epidemiology of Cholera in Conjunction with the Genomics of Vibrio cholerae. Front Public Health. 2019; 7: 203. doi: 10.3389/fpubh.2019.00203
2.    WHO. Cholera. World Health Organization fact sheet .2017. http://www.who.int/mediacentre/factsheets/fs107/en/ (Accessed oct 26, 2020).
3.    WHO. Cholera 2017. Weekly Epidemiol Rec. 2018; 93:489–500.
4.    Saha D. Karim MM. Khan WA. Ahmed S. Salam MA.Bennish ML. Single-dose azithromycin for the treatment of cholera in adults. N Engl J Med. 2006; 354: 2452-2462.doi: 10.1056/NEJMoa054493. PMID: 16760445.
5.    Clemens JD. Nair GB. Ahmed T. Qadri F. Holmgren J. Cholera. Lancet. 2017; 390: 1539-1549. DOI:https://doi.org/10.1016/S0140-6736(17)30559-7
6.    Verma J. Bag S. Saha B. Kumar P. Ghosh TS. Dayal M. Senapati T. et al. Genomic plasticity associated with antimicrobial resistance in Vibrio cholerae. Proc Natl Acad Sci USA. 2019; 116 (13): 6226-6231.https://doi.org/10.1073/pnas.1900141116
7.    Wozniak RA.Waldor MK. Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flow. Nat Rev Microbiol. 2010; 8: 552-563.doi: 10.1038/nrmicro2382.
8.    Bhowmick TS. Koley H. Das M. Saha DR. Sarkar BL. Pathogenic potential of vibriophages against an experimental infection with Vibrio cholerae O1 in the RITARD model. Int J Antimicrob Agents. 2009; 33(6): 569-573.doi: 10.1016/j.ijantimicag.2008.10.034.
9.    Jaiswal A. Koley H. Ghosh A. Palit A. Sarkar B. Efficacy of cocktail phage therapy in treating Vibrio cholerae infection in rabbit model. Microbes Infect. 2013; 15(2): 152-156.doi: 10.1016/j.micinf.2012.11.002.
10.    Jaiswal A. Koley H. Mitra S. Saha DR. Sarkar B. Comparative analysis of different oral approaches to treat Vibrio cholerae infection in adult mice. Int J Med Microbiol. 2014; 304(3-4): 422-430.doi: 10.1016/j.ijmm.2014.02.007.
11.    Bhandare S. Colom J. Baig A. Ritchie JM. Bukhari H. et al. Reviving Phage Therapy for the Treatment of Cholera. J Infect Dis. 2019; 219(5): 786-794.DOI: 10.1093/infdis/jiy563
12.    Gutiérrez B Domingo-Calap P. Phage Therapy in Gastrointestinal Diseases. Microorganisms. 2020; 8: 1420.doi: 10.3390/microorganisms8091420.
13.    Tarh JE. A Review on Diagnostic Methods for the Identification of Vibrio cholerae. J Adv Med Med Res. 2020; 32: 136-164.DOI: 10.9734/jammr/2020/v32i830474
14.    CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute. 2020.
15.    Kumari S. Harjai K. Chhibber S. Evidence to support the therapeutic potential of bacteriophage Kpn5 in burn wound infection caused by Klebsiella pneumoniae in BALB/c mice. J Microbiol Biotechnol. 2010; 20:935–941. doi: 10.4014/jmb.0909.09010.
16.    Tan GH. Nordin MS. Napsiah AB. Isolation and characterization of lytic bacteriophages from sewage water (Pengasing and an pencirianbakteriofajdaripada air kumbahan). J Trop AgricFd Sci. 2008; 36(2): 1-5.
17.    Jassim SA. Abdulamir AS. Abu Bakar F. Novel phage-based bioprocessing of pathogenic Escherichia coli and its biofilms. World J Microbiol Biotechnol. 2011; 28(1): 47-60.doi: 10.1007/s11274-011-0791-6.
18.    Knezevic P. Petrovic O. A colorimetric microtiter plate method for assessment of phage effect on Pseudomonas aeruginosa biofilm. J Microbiol Methods. 2008; 74 (2-3):114-118.doi: 10.1016/j.mimet.2008.03.005.
19.    Taati Moghadam M. Khoshbayan A. Chegini Z. Farahani I. Shariati A. Bacteriophages, a New Therapeutic Solution for Inhibiting Multidrug-Resistant Bacteria Causing Wound Infection: Lesson from Animal Models and Clinical Trials. Drug Des Devel Ther. 2020; 14: 1867-1883. doi: 10.2147/DDDT.S251171.
20.    Rakhuba DV. Kolomiets EI. Szwajcer E. Novik GI. Bacteriophage Receptors, Mechanisms of Phage Adsorption and Penetration into Host Cell. Pol J Microbiol. 2010; 59(3): 145-155.
21.    Sneha Bokadia G. Gopinath P. Bacteriophage in Human Body A –Systematic Review. Research J. Pharm. and Tech 2017; 10(4):1204-1208.DOI: 10.5958/0974-360X.2017.00216.5
22.    Randall-Hazelbauer L. Schwartz M. Isolation of the bacteriophage lambda receptor from Escherichia coli. J Bacteriol. 1973; 116: 1436-1446.doi: 10.1128/jb.116.3.1436-1446.1973.
23.    Yu F. Mizushima S. Roles of lipopolysaccharide and outer membrane protein OmpC of Escherichia coli K12 in the receptor function for bacteriophage T4. J Bacteriol. 1982; 51: 423-434.doi:10.1128/jb.151.2.718-722.1982.
24.    Hashemolhosseini S. Holmes Z. Mutschler B. Henning U. Alterations of receptor specificities of coliphages of the T2 family. J Mol Biol. 1994; 240: 105- 110.https://doi.org/10.1006/jmbi.1994.1424.
25.    Kaabi SAG. Musafer HK. An experimental mouse model for phage therapy of bacterial pathogens causing bacteremia. Microb Pathog. 2019; 137: 103770.doi: 10.1016/j.micpath.2019.103770.
26.    Kaabi SAG. Musafer HK. New Phage cocktail against infantile Sepsis bacteria. Microb Pathog. 2020;148: 104447.doi: 10.1016/j.micpath.2020.104447.
27.    Kaabi SAG. Musafer HK. Novel Phage Cocktail for the Treatment of Bacteria Causing Chronic Suppurative Otitis Media. Trop J Nat Prod Res. 2020; 4: 680-686.https://doi.org/10.26538/tjnpr/v4i10.4.
28.    Muralidharan V. Deecaraman M. Remote Sensing for Recognition of Vibrio Cholerae . Research J. Pharm. and Tech 2017; 10(10):3521-3523.DOI: 10.5958/0974-360X.2017.00633.3.

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