Author(s): Budi Arief Waskito, Djanggan Sargowo, Umi Kalsum, Askandar Tjokroprawiro

Email(s): budiariefwaskito@gmail.com

DOI: 10.52711/0974-360X.2022.00398   

Address: Budi Arief Waskito1,2*, Djanggan Sargowo3, Umi Kalsum4, Askandar Tjokroprawiro5
1Doctoral Program of Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia.
2Department of Internal Medicine, Faculty of Medicine, Wijaya Kusuma University, Surabaya, East Java, Indonesia.
3Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Brawijaya/Dr. Saiful Anwar General Hospital, Malang, East Java, Indonesia.
4Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia.
5Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo Hospital, Surabaya, East Java, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 6,     Year - 2022


ABSTRACT:
Ipomoea batatas L purple variant is a natural source of bioactive compounds with strong antioxidant activity and has long been used in folk medicine. This study aimed to evaluate the active compounds, antioxidant, and anti-inflammatory activities of Ipomoea batatas L purple variant leaf extract in rats fed a high-fat diet. The presence of active compounds in the Ipomoea batatas L purple variant was determined by liquid chromatography high resolution mass spectrometry (LC-HRMS). The rats were divided into five experimental groups of six animals in each group. Namely standard diet/normal group (SD), high-fat diet group (HFD), and HFD plus extracts (625; 1250; or 2500 mg/kg) groups for twelve weeks. The effect of leaf extract on antioxidant activity was analyzed using the DPPH assay, while oxidative stress and anti-inflammatory analysis were performed by immunohistochemistry. LC-HRMS analysis showed six active compounds were identified from Ipomoea batatas L. Besides, there is a reduction in oxidative stress through malondialdehyde (MDA) expression on the oral supplementation with Ipomoea batatas leaf purple variant extract doses of 1250 and 2500 mg/kg body weight. Meanwhile, all extract doses can significantly increase antioxidant activities such as nuclear factor-erythroid 2 related factor 2 (Nrf-2) expression, superoxide dismutase 2 (SOD2) expression, and decrease pro-inflammatory such as tumor necrosis factor alpha (TNF-a) expression in the aorta. Taken together, these findings provide a basis for the recommendation of compounds from the supplementation of an Ipomoea batatas L purple variant leaf extract can be an alternative herbal therapy to inhibit the harmful effects of a high-fat diet.


Cite this article:
Budi Arief Waskito, Djanggan Sargowo, Umi Kalsum, Askandar Tjokroprawiro. The Role of Ipomoea batatas Leaves Extract as a Potent Antioxidant and Anti-inflammatory in Rats Fed High-fat Diet. Research Journal of Pharmacy and Technology. 2022; 15(6):2395-1. doi: 10.52711/0974-360X.2022.00398

Cite(Electronic):
Budi Arief Waskito, Djanggan Sargowo, Umi Kalsum, Askandar Tjokroprawiro. The Role of Ipomoea batatas Leaves Extract as a Potent Antioxidant and Anti-inflammatory in Rats Fed High-fat Diet. Research Journal of Pharmacy and Technology. 2022; 15(6):2395-1. doi: 10.52711/0974-360X.2022.00398   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-6-2


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