Author(s): Kamran Javed Naquvi, S. H. Ansari, Afrin Salma, Javed Ahamad, Shehla Najib


DOI: 10.52711/0974-360X.2022.00393   

Address: Kamran Javed Naquvi1,2*, S. H. Ansari2, Afrin Salma3, Javed Ahamad4, Shehla Najib5
1Department of Pharmacognosy and Phytochemistry, Faculty of Pharmaceutical Sciences, Rama University, Mandhana, Kanpur, Uttar Pradesh, India.
2Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
3Translam Institute of Pharmaceutical Education and Research, Meerut, Uttar Pradesh, India.
4Department of Pharmacognosy, Faculty of Pharmacy, Tishk International University, Erbil, Kurdistan Region, Iraq.
5Department of Pharmacognosy, Faculty of Pharmacy, King Khalid University, Abha, Kingdom of Saudi Arabia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 5,     Year - 2022

Trachyspermum ammi L. Sprague syn. Carum copticum Benth. & Hook, commonly known as ajwain, is an annual aromatic and herbaceous plant of the family Apiaceae. The fruits of T. ammi are native to Egypt and widely cultivated in Iraq, Iran, Afghanistan, Pakistan, and distributed throughout India. Ajwain is commercially cultivated in Rajasthan, Madhya Pradesh, Andhra Pradesh, Gujarat (Surendranagar, Saurashtra region), Maharashtra, Uttar Pradesh and other states also. Traditionally the plant is used in Ayurvedic and Unani formulations for the treatment of various disorders like flatulence, dyspepsia, diarrhoea, abdominal pains, piles, and bronchial problems, lack of appetite, galactogogue, asthma and amenorrhoea. It has been reported to possess many pharmacological bioactivities like antimicrobial, antioxidant, hypolipidaemic, antihypertensive, antispasmodic, antiasthmatic, antitussive, and many more. This review summarizes the reported traditional benefits, phytochemical and pharmacological studies of T. ammi.

Cite this article:
Kamran Javed Naquvi, S. H. Ansari, Afrin Salma, Javed Ahamad, Shehla Najib. A Review on Phytochemical Investigations and Biological Activities of Trachyspermum ammi (L.) Sprague. Research Journal of Pharmacy and Technology. 2022; 15(5):2364-0. doi: 10.52711/0974-360X.2022.00393

Kamran Javed Naquvi, S. H. Ansari, Afrin Salma, Javed Ahamad, Shehla Najib. A Review on Phytochemical Investigations and Biological Activities of Trachyspermum ammi (L.) Sprague. Research Journal of Pharmacy and Technology. 2022; 15(5):2364-0. doi: 10.52711/0974-360X.2022.00393   Available on:

1.    Anonymous. The Ayurvedic Pharmacopoeia of India. Government of India, Ministry of Health and Family Welfare, Department of Ayurveda, Yoga-Naturopathy, Unani, Siddha and Homoeopathy (AYUSH); New Delhi. 1990; 1(1), 1st edn., 170-171.
2.    Anonymous. The Wealth of India, A dictionary of Indian raw materials and industrial products. Publications and Information Directorate, CSIR, New Delhi. 2013; 10, 267-272.
3.    Lim TK. Trachyspermum ammi. In: edible medicinal and non-medicinal plants. Springer, Dordrecht. 2013; 5: 60-61.
4.    Asif HM. Sultana S. Akhtar N. A panoramic view on phytochemical, nutritional, ethanobotanical uses and pharmacological values of Trachyspermum ammi Linn. Asian Pacific Journal of Tropical Biomedicine. 2014; 4(2): 545-553. DOI:
5.    Chatterjee A. Chandraprakashi S. The treatise of Indian medicinal plants. Publication and Information Directorate, CSIR, New Delhi. 1995; 4: 45-47.
6.    Singh VK. Govil JN. Arunachalam C. Recent progress in medicinal plants. Houstan Texas,USA. 2007; 17.
7.    Ranjan B. Manmohan S. Singh SR. Singh RB. Medicinal uses of Trachyspermum ammi: A review. Pharmaceutical Research. 2011; 5(2): 247-258.
8.    Krishnamoorthy V. Madalageri MB. Bishop weeds (Trachyspermum ammi): an essential crop for north Karnataka. Journal of Medicinal and Aromatic Plants Science. 1999; 21(4): 996- 998.
9.    Ananth NR. Jayaram PCK. Text Book of Microbiology. 1990; 4:578-585.
10.    Chauhan B. Kumar G. Ali M. Composition of volatile oil Carum copticum Benth. & Hook. fruits. International Research Journal of Pharmacy. 2012; 3(7): 131-132.
11.    Malhotra SK. Vijay OP. Ajwain. In: Peter KV (Ed.). Handbook of Herbs and Spices. Wood Head Publishing Ltd., Cambridge, England, 2002; 107-116.
12.    Zcan MM. Akbulut M. Estimation of minerals, nitrate and nitrite contents of medicinal and aromatic plants as spices, condiments and herbal tea. Food Chemistry. 2007; 106: 852-858. DOI:
13.    Duke JA. Handbook of Phytochemical Constituents of GRAS Herbs and Other Economic Plants. CRC Press, Boca Raton, 1992; 46-47.
14.    Mohagheghzadeh A. Faridi P. Ghasemi Y. Carum copticum Benth. & Hook., essential oil chemotypes. Food Chemistry. 2007; 100: 1217-1219. DOI:
15.    Khajeh M. Yamini Y. Sefidkon F. Bahramifar N. Comparison of essential oil composition of Carum copticum obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chemistry. 2004; 86(4): 587-591. DOI:
16.    Eftekhari M. Hoseinsalari A. Mansourian M. Farjadmand F. Ardekani MRS. Sharifzadeh M. Hassanzadeh G. Khanavi M.  Gholami M. Trachyspermum ammi (L.) Sprague, superb essential oil and its major components on peptic ulcers: in vivo combined in silico studies. DARU Journal of Pharmaceutical Sciences. 2019; 27: 317–327. DOI: 10.1007/s40199-019-00279-y.
17.    Chalchat JC. Ozcan MM. Figueredo G. The composition of essential oils of different parts of laurel, mountain tea, sage and ajowan. Journal of Food Biochemistry. 2011; 35:484-499. DOI:
18.    Anis M. Iqbal M. Antipyretic utility of some Indian plants in traditional medicine. Fitoterapia. 1986; 57: 52-55.
19.    Nagalakshmi S. Shankaracharya NB. Naik JP. Rao LJM. Studies on chemical and technological aspects of ajowan (Trachyspermum ammi (L.) syn. Carum copticum Hiern) seeds. Journal of Food Science and Technology. 2000; 37(3): 277-281. URL:
20.    Lockwood GB. Asghari G. Hakimi B. Production of essential oil constituents by cultured cells of Carum copticum L. Flavour and Fragrance Journal. 2002; 17(6): 456-458. DOI:10.1002/ffj.1129.
21.    Yahara S. Sakamoto C. Nohara T. Niiho Y. Nakajima Y. Ito H. Thymoquinol 5-O-beta-dglucopyranoside, kaempferol 3-O-beta-rutinoside and protocatechuic acid from fruit of Scisandrae chinensis. Shoyakugaku Zasshi. 1993; 47: 420-422.
22.    Gonzalez AG.  Barrea JB.  Diaz JG. Lopez LA.  De-Paz PP. Distribution of secondary metabolite in two subspecies of Todaroa aurea Biochemical Systematics and Ecology. 1988; 16 (7-8): 641-645. DOI: 10.1016/0305-1978(88)90078-6.
23.    Ishikawa T. Sega Y. Kitajima J. Water-soluble constituents of ajowan. Chemical and Pharmaceutical Bulletin. 2001; 49 (7): 840-844. DOI:
24.    Garg SK. Sharma ND. Gupta SR. A phenolic glucoside from the seeds of Carum copticum. Phytochemistry. 1980; 19:  2215-2216. DOI: (00)82231-1.
25.    Garg S. A new glucoside from Trachyspermum ammi. Fitoterapia. 1998; 6: 511-512.
26.    Qureshi AA. Kumar KE. Phytochemical constituents and pharmacological activities of Trachyspermum ammi. Plant Archives. 2010; 10(2): 955-959.
27.    Srivastava M. Baby P. Saxena A. GC-MS investigation and antimicrobial activity of the essential oil of Carum copticum Benth & Hook. Acta alimentaria. 1999; 28(3): 291-295. DOI:
28.    Franchi G. Bovalini L. Martelli P. Ferri S. and Sbardellati E. High performance liquid chromatography analysis of the furanochromones khellin and visnagin in various organs of Ammi visnaga (L.) Lam. at different developmental stages. Journal of Ethnopharmacology. 1985; 14: 203–212. DOI: 10.1016/0378-8741(85)90088-1.
29.    Javed I. Iqbal Z. Rahman ZU. Khan FH. Muhammad F. Aslam B. Ali L. Comparative antihyperlipidaemic efficacy of Trachyspermum ammi extracts in albino rabbits. Pakistan Veterinary Journal. 2006; 26(1): 23-29.
30.    Javed I. Zia-Ur-Rahman. Khan MZ. Muhammad F. Aslam B. Iqbal Z. et al. Antihyperlipidaemic efficacy of Trachyspermum ammi in albino rabbits. Acta Veterinaria Brno. 2009; 78: 229-236. DOI:
31.    Begum S. Aslam M. Bhat JU. Parray SA. Javed K. Antihyperlipidemic activity of Ptychotis ajowan in cholesterol and triton induced hyperlipidemic rats. International Journal of Industrial Pharmacy and Biological Sciences. 2012; 1(1): 101-109. DOI: 10.4103/0253-7613.91875.
32.    Saleem U. Riaz S. Ahmad B. Saleem M. Pharmacological screening of Trachyspermum ammi for antihyperlipidemic activity in triton X-100 induced hyperlipidemia rat model. Pharmacognosy Research. 2017; 9(1): S34-S40. DOI: 10.4103/pr.pr_37_17.
33.    Kaskoos RA. GC/MS profile and in-vitro antidiabetic activity of Cinnamomum zeylanicum Blume.. bark and Trachyspermum ammi (L.) Sprague, seeds. Journal of Essential Oil Bearing Plants. 2019; 22(2): 535-544. DOI:
34.    Surveswaran S. Cai Y. Harold C. Sun M. Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chemistry. 2007; 102: 938-953. DOI:
35.    Anilkumar KR. Saritha V. Khanum F. Bawa SA. Ameliorative effect of ajwain extract on hexachlorocyclohexane-induced lipid peroxdation in rat liver. Food and Chemical Toxicology. 2009; 47: 279-282. DOI: 10.1016/j.fct.2008.09.061.
36.    Khan N. Jamila N. Ejaz R. Nishan U. Kim KS. Volatile oil, phytochemical, and biological activities evaluation of Trachyspermum ammi seeds by chromatographic and spectroscopic methods. Analytical Letter. 2020; 53 (6): 984-1001. DOI:
37.    Gilani AH. Jabeen Q. Ghayur MN. Janbaz KH. Akhtar MS. Studies on the antihypertensive, antispasmodic, bronchodilator and hepatoprotective activities of the Carum copticum seed extract.  Journal of Ethnopharmacology. 2005; 98(12): 127-135. DOI: 10.1016/j.jep.2005.01.017.
38.    Siddiqui MJ. Aslam A. Khan T. Comparison and evaluation of different seed extracts of Trachyspermum ammi for immunomodulatory effect on cell-mediated immunity through delayed-type hypersensitivity assay skin thickness method. Journal of Pharmacy and Bioallied Sciences. 2019; 11(1): 43–48. DOI: 10.4103/jpbs.JPBS_174_18.
39.    Alavinezhad A. Boskabady MH. Anti-inflammatory, antioxidant, and immunological effects of Carum copticum L. and some of its constituents. Phytotherapy Research. 2014; 28(12): 1739-48. DOI: 10.1002/ptr.5200.
40.    Sonar PK. Singh R. Saraf SK. Phytochemical, chromatographic and spectroscopic investigation of Carum copticum seeds and their potential as immunomodulatory agents. Pharmaceutical Biology. 2016; 54(3): 494-502. DOI: 10.3109/13880209.2015.1050116.
41.    Shahidi B. Evaluation of antibacterial properties of some medicinal plants used in Iran. Journal of Ethnopharmacology. 2004; 94: 301-305. DOI: 10.1016/j.jep.2004.06.007.
42.    Gurinder JK. Daljit SA. In vitro antibacterial activity of three plants belonging to the family Umbelliferae. International Journal of Antimicrobial Agents. 2008; 31: 380-399. DOI: 10.1016/j.ijantimicag.2007.11.007.
43.    Syed FHZ. Yamada K. Kadowaki M. Khan U. Sugiyama T. Bactericidal activity of medicinal plants, employed for the treatment of gastrointestinal ailments, against Helicobacter pylori. Journal of Ethnopharmacology. 2009; 121: 286-291. DOI: 10.1016/j.jep.2008.11.001.
44.    Sharker SMD. Shahid IJ. Assessment of antibacterial and cytotoxic activity of some locally used medicinal plants in Sundarban mangrove forest region. African Journal of Pharmacy and Pharmacology. 2010; 4(2): 66-69.
45.    Siripornvisal S. Antifungal activity of ajowan oil against Fusarium oxysporum. KMITL Science and Technology Journal. 2010; 10(2): 45-51.
46.    Dwivedi SK. Singh KP. Fungi toxicity of some higher plant products against Macrophmina phascolina (Tassi) Gold. Flavour and Fragrance. 1998; 13(6): 397. DOI:<397::AID-FFJ762>3.0.CO;2-L.
47.    Noorjahan. Ahmad M. Mehjabeen. M. Zia-ul-Haq. Alam SM. Qureshi M. Antimicrobial screening of some medicinal plants of Pakistan.  Pakistan Journal of Botany. 2010; 42(6): 4281-4284.
48.    Wadhwa S. Bairagi M. Bhatt G. Panday M. Porwal A. Antimicrobial activity of essential oils of Trachyspermum ammi. Int. J. Pharmaceut. Biol. Arch., 2010; 1(2): 131-133.
49.    Goudarzi GR. Saharkhiz MJ. Sattari M. Zomorodian K. Antibacterial activity and chemical composition of ajowan (Carum copticum Benth. & Hook) essential oil. Journal of Agricultural Science and Technology. 2011; 13: 203-208.
50.    Moein MR. Zomorodian K. Pakshir K. Yavari F. Motamedi M. Zarshenas MM. Trachyspermum ammi (L.) sprague: chemical composition of essential oil and antimicrobial activities of respective fractions. Evidence-Based Complementary and Alternative Medicine. 2015; 20(1): 50-56. DOI: 10.1177/2156587214553302.
51.    Hejazian SH. Bagheri SM. Safari F. Spasmolytic and anti-spasmodic action of Trachyspermum ammi essence on rat's ileum contraction. North American Journal of Medicine and Science. 2014, 6(12): 643–647. DOI: 10.4103/1947-2714.147982
52.    Boskabady MH. Alizadeh M. Jahanbin B. Bronchodilatory effect of Carum copticum in airways of asthmatic patients. Therapie. 2007; 62(1): 23-29. DOI: 10.2515/therapie:2007007.
53.    Boskabady MH. Jandaghi P. Kiani S. Hasanzadeh L. Antitussive effect of Carum copticum in guinea pigs. Journal of Ethnopharmacology. 2005; 97(1): 79-82. DOI: 10.1016/j.jep.2004.10.016.
54.    Boskabady MH. Moetamedshariati V. Bronchodilatory and anti-cholinergic effects of Carum Copticum on isolated guinea-pig tracheal chain. European Respiratory Journal. 1996; 23: 28s.
55.    Platel K. Srinivasan K. Studies on the influence of dietary spices on food transit time in experimental rats. Nutrition research. 2001; 21: 1309-1314. DOI:
56.    Zaidi SFH. Yamad K. Kadowaki M. Khan U. Sugiyama M. Bactericidal activity of medicinal plant, employed for the treatment for gastrointestinal ailments, against Helicobacter pylori. Journal of Ethnopharmacology. 2009; 121: 286-291. DOI: 10.1016/j.jep.2008.11.001
57.    Dashti-Rahmatabadi MH. Hejazian SH. Morshedi A. Rafati A. The analgesic effect of Carum copticum extract and morphine on phasic pain in mice. Journal of Ethnopharmacology. 2007; 109: 226-228. DOI:
58.    Thangam. C. Dhananjayam R. Antinflammatory potential of the seeds of Carum copticum Linn. Indian Journal of Pharmacology. 2003; 35: 388-391.
59.    Qamar N. John P. Bhatti A. Toxicological and anti-rheumatic potential of Trachyspermum ammi derived biogenic selenium nanoparticles in arthritic balb/c mice. International Journal of Nanomedicine. 2020; 15: 3497-3509. DOI: 10.2147/IJN.S243718.
60.    Hussein G.  Muvashiro H. Nakamura N. Hattori M. Kukiuchi N. Shimotohno K. Inhibitory effects of Sudanese medicinal plant extract on hepatitis C virus (HCV) protease. Phytotherapy Research, 2000; 14(7): 510-516. DOI: 10.1002/1099-1573(200011)14:7<510::aid-ptr646>;2-b.
61.    Roy S. Chaurvedi P. Chowdhary A. Evaluation of antiviral activity of essential oil of Trachyspermum Ammi against Japanese encephalitis virus. Pharmacognosy Research, 2015; 7(3): 263-267. DOI: 10.4103/0974-8490.157977.
62.    Choubey MK. Fumigant toxicity of essential oils from some common spices against pulse bettle, Callosobruchus chinensis (Coleoptera: Bruchidae.). Journal of oleo science. 2008; 57(3): 171-179. DOI: 10.5650/jos.57.171.
63.    Mathew N. Misra-Bhattacharya S. Perumal V. Muthuswamy K. Antifilarial lead molecules isolated from Trachyspermum ammi. Molecules. 2008; 13: 2156-2168. DOI:
64.    Lateef M. Iqbal Z. Rauf U. Jabbar A. Anthelmintic activity of Carum copticum seeds against gastro-intestinal nematodes of sheep. Journal of Animal and Plant Sciences. 2006; 16(1-2): 34-37.
65.    Kwon P. Junheon K. Sang-Gil L. Nematicidal activity of plant essential oils and components from Ajwain (Trachyspermum ammi), Allspice (Pimenta dioica) and Litsea (Litsea cubeba) against pine wood nematode (Bursaphelenchus Xylophilus). Journal of Nematology. 2007; 39(3): 275-279. PMID: 19259498.
66.    Kostyukovsky M. Rafaeli A. Gileadi C. Demchenko N. Shaaya E. Activation of octopaminergic receptors by essential oil constituents isolated from aromatic plants: Possible mode of action against insect pests. Pest Management Science. 2002; 58: 1101-1106. DOI: 10.1002/ps.548
67.    Tamurab T. Iwamoto H. Thymol- a classical small molecule compound that has a dual effect (potentiating and inhibitory) on myosin. Biochemical and Biophysical Research Communications. 2004; 18: 786-789. DOI: 10.1016/j.bbrc.2004.04.085.
68.    Jabbar A. Iqbal Z. Khan MN. In vitro anthelmintic activity of Trachyspermum ammi seeds. Pharmacognosy magazine. 2006; 2, 126-129.
69.    Apte AK. Khot VS. Biradar NS. Patil SB. Anthelmintic activity of Trachyspermum ammi extract. International Journal of Pharmacy and Pharmaceutical Sciences. 2014; 6(1), 464-466.
70.    Srivastava KC. Extract of spice-omum (Trachyspermum ammi) shows antiaggregatory effects and alters arachidonic acid metabolism in human platelets. Prostaglandins, Leukotrienes and Essential Fatty Acids. 1988; 33(1): 1-6. DOI: 10.1016/0952-3278(88)90115-9.
71.    Boskabady MH. Shaikhi J. Inhibitory effect of Carum compticum on histamine (H1) receptors of isolated guinea-pig tracheal chains. Journal of Ethnopharmacology. 2000; 69: 217- 227. DOI: 10.1016/s0378-8741(99)00116-6.
72.    Nath D. Sethi N. Srivastav S. Jain AK. Srivastava R. Survey on indigenous medicinal plants used for abortion in some districts of Uttar Pradesh. Fitoterapia. 1997; 68(3): 223-225.
73.    Kaur H. Estrogenic activity of some herbal galactogogue constituents. Indian Journal of Animal Nutrition. 1998; 15(3): 232-234.
74.    Kumar SM. Reddy R. Manasa G. Vanaja P. Sirisha G. Astalakshmi N. Antifertility effect of Trachyspermum ammi (Linn) Sprague fruits on male rats. International Journal of Pharmaceutical and Biological Archive. 2011; 2(2): 705-709.
75.    Sankpal S. Pathade KS. Kondawar M. Coffea arabica: Herbal drug on Global health issue-obesity. Research Journal of Pharmacognosy and Phytochemistry. 2020; 12(4): 201-206. DOI: 10.5958/0975-4385.2020.00034.5
76.    Tripathi K. Herbs for Obesity: A Review. Research Journal of Pharmacognosy and Phytochemistry.  2009; 1(2): 103-108.
77.    Rohini A. Doss DVA. Anti-lipidemic effect of Alternanthera sessilis Linn in diabetic rats. Research Journal of Pharmacognosy and Phytochemistry. 2010; 2(2): 169-170.
78.    Garg N. Kumar S. Prakash S. Sharma M. Nagar D. Role of yava and madhu in prevention and control of diabetes mellitus. Research Journal of Pharmacognosy and Phytochemistry. 2018; 10(1): 73-76. DOI: 10.5958/0975-4385.2018.00012.2.
79.    Ravichandiran V. Nirmala S. A systematic review on antidiabetic medicinal plants. Research Journal Pharmacognosy and Phytochemistry 2013; 5(3): 155-168.
80.    Murugan M. Uma MRC. Vedhapal JS. Babu AD. Umasankar K. Hypoglycemic activity of Erythrina varigata in alloxan induced diabetic mice. Research Journal Pharmacognosy and Phytochemistry. 2010; 2(1): 89-90.

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