Author(s): Mokhamad Fahmi Rizki Syaban, Nabila Erina Erwan, Muhammad Rafif Raihan Syamsuddin, Fatimah Az Zahra, Faradilah Lukmana Sabila


DOI: 10.52711/0974-360X.2022.00324   

Address: Mokhamad Fahmi Rizki Syaban1*, Nabila Erina Erwan2, Muhammad Rafif Raihan Syamsuddin2, Fatimah Az Zahra2, Faradilah Lukmana Sabila2
1Faculty of Medicine, Brawijaya Univesity, Malang, 65111 Indonesia.
2Master Program in Biomedical Science, Faculty of Medicine, Brawijaya Univesity, Malang,65111, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 5,     Year - 2022

Beta-lactamase is an enzyme protein that plays a role in the occurrence of antibiotic resistance against Methicillin-resistant Staphylococcus aureus (MRSA) bacteria. This study aims to investigate interactions that occur beta-glucan with Beta-lactamase enzymes and Protein Binding Penicillin-2a (PBP-2a). In this study, the bioinformatics approach or in-silico method was conducted to determine the molecular interactions that occurred computationally. The protein used was Beta-lactamase protein (4ooy), and Protein Binding Penicillin-2a (6h50) obtained from the Protein Data Bank. Beta-glucan as ligand obtained from the PubChem web server. Protein stabilization was carried out to adjust to the body's physiology, carried out using Pymol by removing water atoms and adding hydrogen atoms. Pharma expert web server and Pyrex were used to modulate the interaction between ligand and enzyme. We were analyzed molecular interactions visualization on the molecular complexes generated by docking simulations using the Discovery Studio software. The results showed that beta-glucan has high activity as an antibiotic against Beta-lactamase and PBP-2a. The binding affinity interaction that occurs between Beta-glucan and Beta-lactamase complex interaction was -11.1 kcal/mol, while Beta-glucan and BPP-2a was -8.5 kcal/mol. The interaction bond Beta-glucan and Beta-lactamase was higher than 2s, 5r) -1-Formyl-5 - [(Sulfooxy) amino] piperidine-2-Carboxamid as control ligand. Beta-glucan was predicted to have strong antibacterial properties. However, exploration of beta-glucan compounds and further research to determine the antibacterial effect of beta-glucan against MRSA bacteria.

Cite this article:
Mokhamad Fahmi Rizki Syaban, Nabila Erina Erwan, Muhammad Rafif Raihan Syamsuddin, Fatimah Az Zahra, Faradilah Lukmana Sabila. Insilico Study and Analysis Antibacterial Activity of Beta-glucan against Beta-Lactamase and Protein Binding Penicillin-2A. Research Journal of Pharmacy and Technology. 2022; 15(5):1948-2. doi: 10.52711/0974-360X.2022.00324

Mokhamad Fahmi Rizki Syaban, Nabila Erina Erwan, Muhammad Rafif Raihan Syamsuddin, Fatimah Az Zahra, Faradilah Lukmana Sabila. Insilico Study and Analysis Antibacterial Activity of Beta-glucan against Beta-Lactamase and Protein Binding Penicillin-2A. Research Journal of Pharmacy and Technology. 2022; 15(5):1948-2. doi: 10.52711/0974-360X.2022.00324   Available on:

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