Author(s): S. Subalakshmi, S. S. M. Umamageswari, M. Kalyani

Email(s): subalakshmisankaran8497@gmail.com

DOI: 10.52711/0974-360X.2022.00352   

Address: S. Subalakshmi1*, S. S. M. Umamageswari2, M. Kalyani3
1Tutor, Department of Microbiology, Panimalar Medical College Hospital and Research Institute, Varadharajapuram, Poonamallee, Chennai – 600123.
2Professor, Saveetha Medical College Hospital, Saveetha Nagar, Thandalam, Chennai - 602 105.
3Professor and Head, Department of Microbiology, Panimalar Medical College Hospital and Research Institute, Varadharajapuram, Poonamallee, Chennai – 600123.
*Corresponding Author

Published In:   Volume - 15,      Issue - 5,     Year - 2022


ABSTRACT:
Presence of antibacterial activity in urine will reduce the bacterial load in culture plate, leading to “Insignificant Bacteriuria” as a false positive result. UABA (Urine antibacterial substance assay) is therefore performed to make accurate findings.The aim of the study was to check for the antibacterial activity of urine samples obtained from patients in a tertiary care hospital. Total of 100 urine samples were collected and preliminary tests were performed. Antibiotic susceptibility test was performed using Kirby-Bauer disc diffusion assay with the urine samples against the ATCC strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Zone formation was compared with the growth in the urine culture plate and the corresponding patients antibiotic history. The findings were, out of 100 samples 23 samples were true positive and 50 samples were true negative, 35 samples were UABA positive with culture negativity 8 samples were UABA positive and culture positive. Sensitivity and specificity of the test was found to be 76.66% and 71.42% respectively. This study helps in understanding the importance of performing urine culture and sensitivity testing before starting the antibiotic therapy. False negative results can be reduced to promote flawless diagnosis and speedy recovery.


Cite this article:
S. Subalakshmi, S. S. M. Umamageswari, M. Kalyani. Antibacterial Screening of Urine Culture using Urine Antibacterial Substance Assay (UABA). Research Journal of Pharmacy and Technology. 2022; 15(5):2122-6. doi: 10.52711/0974-360X.2022.00352

Cite(Electronic):
S. Subalakshmi, S. S. M. Umamageswari, M. Kalyani. Antibacterial Screening of Urine Culture using Urine Antibacterial Substance Assay (UABA). Research Journal of Pharmacy and Technology. 2022; 15(5):2122-6. doi: 10.52711/0974-360X.2022.00352   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-5-34


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