Ali Alrabie, Arwa Al-Dhreai, Inas Al-Qadsy, Vidya Pradhan, Mazahar Farooqui
Ali Alrabie1*, Arwa Al-Dhreai1, Inas Al-Qadsy1, Vidya Pradhan2, Mazahar Farooqui1
1Department of Chemistry, Maulana Azad College of Arts, Science and Commerce, Aurangabad (M.S) - India.
2Dr. Rafiq Zakaria College for Women, Aurangabad (M.S) - India.
Volume - 15,
Issue - 5,
Year - 2022
Objectives: The present study was designed to identify and screen phytochemicals present in methanolic extract of Sapindus emarginatus seed kernel, to evaluate its antimicrobial and antioxidant activity and to search for dihydropteroate synthase (3TYE) enzyme inhibitors through molecular docking study. Methods: Phytochemicals present in methanol extract were screened using standard protocol. Identification of phytochemicals was carried out using GC-MS. DPPH assay and Microbroth dilution assay were used to evaluate antioxidant and antimicrobial activity respectively. Molecular Operating Environment (MOE) was used for molecular docking. Results: Preliminary phytochemicals screening of extract revealed the presence of alkaloids, carbohydrates, flavonoids, glycosides, phenols and tannins, terpenoids, saponins and fat & oil. GC-MS study was showed 12 peaks indicating the presence of 12 different phytoconstituents. The extract exhibited very good inhibition (MIC= 200µg/mL) against S. pyogenus compared with inhibition of standard drug ampicillin (MIC= 100µg/mL). Molecular docking result revealed that 4H-1-Benzopyran-4-one, 2-[3,4-dihydroxyphenyl]-5-hydroxy-3,7-dimethoxy bind effectively to the active site residues of dihydropteroate synthase (3TYE) with docking score of -6.53kcal/mol. Conclusion: Phytochemicals were investigated (Phytochemicals screening and GC-MS analysis) are responsible of antimicrobial or antioxidant activity of Sapindus emarginatus seed kernel.
Cite this article:
Ali Alrabie, Arwa Al-Dhreai, Inas Al-Qadsy, Vidya Pradhan, Mazahar Farooqui. Phytochemical Screening, GC-MS Analysis, Molecular Docking Study and Evaluation of Antioxidant and Antimicrobial Activity of Sapindus emarginatus Seed Kernel. Research Journal of Pharmacy and Technology. 2022; 15(5):2117-1. doi: 10.52711/0974-360X.2022.00351
Ali Alrabie, Arwa Al-Dhreai, Inas Al-Qadsy, Vidya Pradhan, Mazahar Farooqui. Phytochemical Screening, GC-MS Analysis, Molecular Docking Study and Evaluation of Antioxidant and Antimicrobial Activity of Sapindus emarginatus Seed Kernel. Research Journal of Pharmacy and Technology. 2022; 15(5):2117-1. doi: 10.52711/0974-360X.2022.00351 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-5-33
1. Oladeji O. The characteristics and roles of medicinal plants: Some important medicinal plants in Nigeria. Natural Products: An Indian Journal. 2016; 12(3): 1-8.
2. Mathur S. Hoskins C. Drug development: Lessons from nature (Review). Biomedical reports 2017; 6: 612-614.doi.org/10.3892/br.2017.909.
3. Gunatilaka A. Natural products from plants-Associated Microorganisms: Distribution, structural diversity, bioactivity, and implications of their occurrence. Journal of Natural products 2006; 69(3): 509-526.doi.org/10.1021/np058128n.
4. Goyal B. Goyal R. Mehta A. Phyto-pharmacology of Achyranthes aspera: A review. Pharmacognosy Review 2007; 1(1): 143-150.
5. Khan R. Natural products chemistry: The emerging trends and prospective goals. Saudi pharmaceutical journal 2018; 26: 739-753.doi.org/10.1016/j.jsps.2018.02.015
6. Sekhar R. Seetharam T. Amrutha P. Maharshi V. Presley A. Swathi M. In-vitro and in-vivo anti-inflammatory activity of Sapindus emarginatus leaf extract. Research journal of pharmaceutical, biological and chemical sciences 2014; 5(3): 2050-2058.
7. Sathiya M. Muthuchelian K. Phytochemical investigation and antibacterial screening of ethanolic leaf extract of Sapindus emarginatus Vahl. Ethnobotanical leaflets. 2008; 12: 891-895.
8. Patil M. Khan P. Antibacterial activity of leaves of Nyctanthusarbor-tristis L, Hibiscus rosa-sinensis L and Sapindus emarginatus Vahl. Science park research journal, 2015; 2(31): 1-4.doi.org/ 10.9780/23218045/1202013/49
9. Ramamurthy V. Sathiyadevi M. Preliminary Photochemical Screening of Methanol Extract of Indigofera trita linn. Journal of Molecular Histology and Medical Phsiology, 2017; 2(1): 1-5.doi.org/ 10.4172/2329-9029.1000184.
10. Anitha R. Sudarsanam D. Phytochemicals screening and in-vitro antioxidant activity of Cissus quadrangualris. Asian J. Research Chem. 2010; 3(4): 876-878.
11. Basaar Ola. Alrabie A. Fatema S. Farooqui M. Screening of supercritical fluid extract of Gymnmea sylvestre R. Br. roots for phytochemical and pharmacological analysis. International journal of PharmTech research 2017; 10(3): 131-138.doi.org/ 10.20902/IJPTR.2017.10319.
12. Alrabie A. Bassar O. Farooqui M. Gas Chromatography-Mass Spectrometry, Inductively Coupled Plasma Mass Spectrometry investigation, and antimicrobial screening of Caesalpinia bonducella (L.) Roxb seed kernel. Asian journal of pharmaceutical and clinical research 2019; 12(4): 315-319.doi.org/10.22159/ajpcr.2019.v12i4.32067
13. Ola B. Samreen F. Mohammed M. Mazahar F. Evaluation and phytochemical and pharmacological properties of Cichorium intybus (L) Based on supercritical fluid extract. Research Journal of pharmaceutical, biological and chemical Sciences, 2017; 8: 1857-1866.
14. Basa´ar O. Fatema S. Alrabie A. Mohsin M. Farooqui M. Supercritical carbon dioxide extraction of Triognella foenum graecum linn seeds: Determination of bioactive compounds and pharmacological analysis. Asian Pacific Journal of Tropical Biomedicine 2017; 7(12): 1085-1091.doi.org/10.1016/j.apjtb.2017.10.010
15. Alrabie A. Bass΄ar O. Al-qadsy I. Farooqui M. GC-MS analysis, HPTLC fingerprint profile and DPPH free radical scavenging assay of methanol extract of Martynia annua Linn seeds. International journal of pharmacy and pharmaceutical sciences 2019; 11(6): 16-22. doi.org/10.22159/ijpps.2019v11i6.32206
16. Eltayeb E. Faizul A. Mahmoud H. et al. Zerumbone binding to estrogen receptor: an in silico investigation. Journal of receptors and signal transduction 2018; 38(4): 1-10. doi.org/10.1080/10799893.2018.1531886
17. Chinnasamy S. Phytochemicals and ir-spectral investigation of leaf extracts of Sapindus emarginatus vahl. Life science archives 2018; 4(3): 1369-1382.doi.org/10.22192/lsa.2018.4.3.4
18. Asghar S. Rehman H. Choudahry I. Rhaman A. Gas chromatography-mass spectrometry (GC-MS) analysis of petroleum ether extract (oil) and bio-assays of crude extract of Iris germanica. International journal of genetics and molecular biology 2011;3(7): 95-100.doi.org/10.5897/IJGMB.9000024.
19. Hase G. Deshmukh K. Pokharkar R. Gaje T. Phatanagre N. Phytochemical studies on Nerium oleander L. using GC-MS. International journal of pharmacognosy and phytochemical research 2017; 9(6): 885-891.doi.org/10.25258/phyto.v9i6.8195
20. Parthipan B. Suky T. Mohan R. GC-MS analysis of phytocomponents in Pleiospermium alatum (Wall. Ex Wight and Arn.) Swingle, (Rutaceae). Journal of pharmacognosy and phytochemistry 2015; 4(1): 216-222.
21. Kadam A. Gaykar B. Phytochemistry of contraceptive plants. Lambert academic publishing. 2018: 1-58.
22. Balouiri M. Sadiki M. Ibnsouda S. Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis 2016; 6(2): 71-79.doi.org/10.1016/j.jpha.2015.11.005
23. Yanping W. Jinrong B. Kai Z. Yina H. et al. Antibacterial activity and membrane-disruptive mechanism of 3-p-trans-Coumaroyl-2-hydroxyquinic acid, a novel phenolic compound from pine needles of Cedrus deodara against Staphylococcus aureus. Molecules. 2016; 21: 1-12.doi.org/10.3390/molecules21081084
24. Ragurkar M. Jain G. Bedmotha A. Khadbadi S. Antioxidant activity of phenolic extract from Ginger (Zingber officinale Roscoe) Rhizome. Asian J. Research Chem. 2009; 2(3): 280-281.
25. Zohra M. Fawazia A. Chemical Composition and antioxidant activity of essential oil from Smyrnium Olusatrum L. Asian J. Research Chem. 2011; 4(2): 217-220.
26. Ankush G. Radhika M. Pooja C. Shubhini A. Saraf. Free radical scavenging activity of novel 5-substituted Arlidene-3-substituted-Benzyl-Thiazolidine-2,4-Diones. Asian J. research Chem.2010; 3(3): 528-530.
27. Sonu J. Anil J. Leena T. Sabulal B. In vitro pharmacological activity of the whole plant Naregamia alata. Asian J. Research Chem. 2012; 5(2): 285-289.
28. Chakrabarty N. Hossain A. Barua J. Kar H. Milonizzaman. et al. Insilico molecular docking of some isolated selected compounds of Lantana camera against Alzheimer’s disease. Biomedical Journal of Scientific and Technical Research 2018; 12(2): 9168-9171.doi.org/ 10.26717/BJSTR.2018.12.002235