Author(s): Madhuri Baghel, Hemant Badwaik, Sangeeta Patil


DOI: 10.52711/0974-360X.2022.00310   

Address: Madhuri Baghel*, Hemant Badwaik, Sangeeta Patil
Rungta College of Pharmaceutical Sciences and Research, Bhilai, Chhattisgarh, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 4,     Year - 2022

Oxalis Carniculata Linn belonging to Oxalidaceae family is a small herb with variety of biological activity. The leaves of Oxalis Carniculata was extracted with different solvents and the fractions were screened for antioxidant potential using in-vitro methods such as 2, 2-Diphenyl-2-Picryl hydrazyl (DPPH) and H2O2 radical scavenging activity, and reducing power assay . Total flavonoid, phenolic content and total antioxidant capacity were determined spectrophotometrically. Among the six different fractions ethanolic fraction exhibited highest total flavonoid content (224.2±0.25 mg/g QAE) and methanolic fraction exhibited highest phenolic content (184.31±0.14 mg/g GAE) and total antioxidant capacity (232.79±0.17mg/g AAE). Further methanolic fraction showed highest DPPH and H2O2 radical scavenging activity with IC50 value 23.43±0.12 and 19.71±0.16 respectively. The major cause of skin photoaging is exposure to ultraviolet light that is coupled with the increased expression of matrix metalloproteinases (MMPs) and decreased collagen synthesis. MMPs (especially MMP-1, MMP-3 and MMP-9) expressions are associated with the decreased elasticity of the dermis due to collagen degradation. In the present study, we have assessed the antioxidant effects of Oxalis Carniculata extract, correlated it with skin photoaging and postulated its mechanism of action by molecular docking study. The molecular docking study of flavones present in O. Carniculata revealed that the phyto-constituents of O. Carniculata species have similar mechanism of binding to that of standard drug with MMP-9 inhibitory action. These results can facilitate the further preclinical models, and clinical study upon skin protective potential against UV-induced skin aging of the O. Carniculata flavones.

Cite this article:
Madhuri Baghel, Hemant Badwaik, Sangeeta Patil. In-vitro antioxidant activity of oxalis carniculata linn and investigation of plausible mechanism of photo-protection by molecular modeling study. Research Journal of Pharmacy and Technology. 2022; 15(4):1843-1. doi: 10.52711/0974-360X.2022.00310

Madhuri Baghel, Hemant Badwaik, Sangeeta Patil. In-vitro antioxidant activity of oxalis carniculata linn and investigation of plausible mechanism of photo-protection by molecular modeling study. Research Journal of Pharmacy and Technology. 2022; 15(4):1843-1. doi: 10.52711/0974-360X.2022.00310   Available on:

1.    Havlik NL, Fitzpatrick TB, Kligman AM, Kligman LH. editors. Fitzpatrick's Dermatology in General Medicine, Vol. 2. McGraw-Hill New York; 1999; pp 1707-1723.
2.    Gilchrest BA, Yaar M. Aging and photoaging of the skin: observations at the cellular and molecular level. British Journal of Dermatology. 1992; 127:25-30.
3.    Uitto J. Connective tissue biochemistry of the aging dermis: age-related alterations in collagen and elastin. Dermatologic Clinics. 1986; 4:433-446.
4.    Schwartz E, Cruickshank FA, Mezick JA, Kligman LH. Topical all-trans retinoic acid stimulates collagen synthesis in vivo. Journal of Investigative Dermatology. 1991; 96: 975-978.
5.    Murphy G, Hembry RM, Hughes CE, Fosang AJ, Hardingham TE: Role and regulation of metalloproteinases in connective tissue turnover. Biochemical Society Transactions. 1990;18:812-815.
6.    Shingleton WD, Hodges DJ, Brick P, Cawston TE: Collagenase a key enzyme in collagen turnover. Biochemistry and Cell Biology. 1996;74:759-775,
7.    Balamurugan G, Arunkumar MP, Muthusamy P, Anbazhagan S. Preliminary Phytochemical Screening, Free radical Scavenging and Antimicrobial activities of Justicia tranquebariensis Linn. Research Journal of Pharmacy and Technology. 2008; 1(2):116-118.
8.    Rayate Y, Shewale S, Patil A, Nitalikar MK, Mohite S. Phytosomes–A Novel Approach in Herbal Drug Delivery System. Asian Journal of  Research in Pharmaceutical Science. 2018; 8(3):151-154.
9.    Badwaik H, Singh MK, Thakur D, Giri TK, Tripathi DK. The Botany, Chemistry, Pharmacological and Therapeutic Application of Oxalis Corniculata Linn– A Review. International Journal of Phytomedicine. 2011;3: 01-08.
10.    Kaur S, Kaur G, Singh J. Phytochemical screening and biological potential of methanolic extract of Oxalis corniculata using different parts of plant. Research Journal of Chemical sciences. 2017;  7(7): 26-32.
11.    Badrul Alam M, Sarowar hossain M, Sultanachaudhary N.  invitro and invivo antioxidant and toxicity evaluation of different fractions of Oxalis carniculata linn.  Journal of pharmacology and toxicology. 2011; 6(4): 337-348.
12.    Vijaya Kumar S., Dhirendra B. Sanghai, Mallikarjuna Rao C., Shreedhara C. S. Antioxidant and antihyperlipidemic activity of Melia azedarach Linn. extracts in rats. Research Journal of Pharmacy and Techology. 20313; 6(11): 1195-1199.
13.    Elias J, Rajesh MG, Anish NP, Ragitha EV, Jayan N. Pharmacognostic Standardization of Solanum melongena var. insanum Linn. Research Journal of Pharmacognosy and Phytochemistry. 2010; 2(5): 364-367.
14.    Kokate C K, Practical Pharmacognosy, Vallabh Prakashan,  Delhi, India,  2000, p. 218.
15.    Chang C, Yang M. Estimation  of  total  flavonoids  content  in  Propolis  by  two  complementary colorimetric methods. Journal of Food and Drug Analysis. 2002; 10: 178–18.
16.    Yu L, Haley S,  Perret J, Harris M, Wilson J , Qian M. Free Radical Scavenging Properties of Wheat Extracts. Journal of Agricultural and Food Chemistry 2002; 50: 1619−1624.
17.    Prieto P, Pineda M. Aguilar M.  Spectrophotometric quantitation of antioxidant capacity through the formation of  a  phosphomolybdenum  complex:  specific  application  to the determination of vitamin E. Analytical Biochemistry. 1999; 269: 337-341.
18.    Barros L, Falcão S. Baptista P. Antioxidant activity of Agaricus sp. mushrooms by chemical, biochemical and electrochemical assays. Food Chemistry. 2008; 111; 61–66.
19.    Oyaizu M. studies on product of browning reaction prepared from glucose amine.  Japanese. Journal of Nutrition. 1986; 44: 307-315.
20.    Shanmugapriya E, Ravichandiran V, Vijey Aanandhi M. Molecular docking studies on naturally occurring selected flavones against protease enzyme of Dengue virus. Research Journal of Pharmacy and Technology. 2016; 9(7):929-932.
21.    Suvarchala NVL, Reddy V, Sneha J, Anarthe, Ganga Raju M, Akhila M, Pooja Raj GB. Molecular docking studies of isolated compounds from Cassia fistula on HMG-COA reductase. Asian Journal of  Research in Chemistry. 2019; 12(2): 89-93.
22.    LakshmananK , Balasubramanian HB, Aiyalu R, Ramasamy A. Molecular Docking Studies of Flavones in Gentianaceae Family against Liver Corrective Targets. Research  Journal of  Pharmacognosy and Phytochemistry. 2019; 11(2):49-53.
23.    Suganya J, Manoharan S, Radha M, Singh N, Francis A. Identification and Analysis of Natural Compounds as Fungal Inhibitors from Ocimum sanctum using in silico Virtual Screening and Molecular Docking. Research Journal of Pharmacy and Technology. 2017; 10(10):3369-3374.
24.    Suganya J, Viswanathan T, Radha M, Marimuthu N. In silico Molecular Docking studies to investigate interactions of natural Camptothecin molecule with diabetic enzymes. Research Journal of Pharmacy and Technology. 2017; 10(9): 2917-2922
25.    Dewangan D, Nakhate KT, Verma VS, Nagori K, Badwaik H, Nair N. Tripathi DK, Mishra A. Synthesis and Molecular Docking Study of Novel Hybrids of 1,3,4-Oxadiazoles and Quinoxaline as a Potent Analgesic and Anti-Inflammatory Agents. Journal of Heterocyclic Chemistry. 2018; 55(12): 2901-2910.
26.    Ahirwar J, Ahirwar D, Lanjhiyana S, Jha AK, Dewangan D, Badwaik H. Analgesic and Anti-inflammatory Potental of Merged Pharmacophore Contaning 1,2,4-triazole and Substituted Benzyl Groups via Thio Linkage. Journal of Heterocyclic Chemistry. 2018; 55(09): 2130-2141.
27.    Ahirwar J, Ahirwar D, Lanjhiyana S, Jha AK, Dewangan D, Badwaik H. Synthesis, Characterization, Molecular Modeling, and Biological Evaluation of 1,2,4-Triazole-pyridine Hybrids as Potential Antimicrobial Agents.  Journal of Heterocyclic Chemistry. 2018; 55(11): 2598-2609
28.    Shravani S. Pawar, Sachin H. Rohane. Review on Discovery Studio: An important Tool for Molecular Docking. Asian Journal of Research in Chemistry.       2021; 14(1):86-88.
29.    Beckmann JS, Beckmann TW, Chen J, Marshal PA, Freeman BA. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide to superoxide. Proceeding of the National Academy of  Sciences of the United States of America. 1990: 87; 1620-1624.
30.    Nagase H,    Visse R,    Murphy G.  Structure and function of  matrix metalloproteinases  and  TIMPs. Cardiovascular Research. 2006: 69; 562–573.

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