Author(s): Lukisiari Agustini, Nurwasis, Aryati, Gatut Suhendro, Winarto, I Ketut Sudiana, Widjiati, Bambang Purwanto

Email(s): nurwasis.unair@gmail.com

DOI: 10.52711/0974-360X.2022.00301   

Address: Lukisiari Agustini1, Nurwasis1*, Aryati2, Gatut Suhendro1, Winarto3, I Ketut Sudiana4, Widjiati5, Bambang Purwanto6
1Department of Ophthalmology, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya 60131, Indonesia.
2Department of Pathology Clinic, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya 60131, Indonesia.
3Faculty of Medicine, Universitas Diponegoro, Semarang 50275, Indonesia.
4Department of Anatomic Pathology, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya 60131, Indonesia.
5Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
6Department of Physiology, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya 60131, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 4,     Year - 2022


ABSTRACT:
Background: The cause of cell death is thought to be due to the pathological apoptotic process in Retinal Ganglion Cells (RGCs), but how the exact mechanism of what is most influential is still not explained. Objective: This study aimed to explain the mechanism of RGCs apoptosis Rattus Norvegicus which is thought to underlie the occurrence of ethambutol toxic optic neuropathy. Methods: A total of 42 male, adult Rattus norvegicus Sprague-Dawley strains were divided into 6 groups with 3 control groups and 3 treatment groups in a randomized design with time series test. The treatment groups were given ethambutol 15 mg/kg/day for each group within 5, 10 and 15 days orally using a gauge.Expressions of SOD2, MDA, PKC d, p53, Cyt c, Caspase 3 and apoptosis were examined by immunohistochemical methods. Results: Ethambutol affected significant decreased expression of SOD2 with p=0.002 in 5 days, p=0.013 in 10 days and p=0.018 in 15 days; significant increased MDA in 5 days with p=0.05, 10 days with p=0.017, 15 days with p=0.002; significant increased p53 in 5 days with p=0.012, 10 days with p=0.002, 15 days with p=0.001; significant increased Cyt c in 5 days with p=0.004, 10 days with p=0.001, 15 days with p=0.001; significant increased Caspase 3 in 5 days with p=0.001, 10 days with p=0.003, 15 days with p=0.001 and apoptosis in 5 days with p=0.001, 10 days with p=0.001, 15 days with p=0.001. Conclusion: The mechanism of apoptosis of RGCs caused by ethambutol was showed via decreased expression SOD2, increased expression of MDA, p 53, Cyt c, Caspase 3 andapoptosis. These biomarkers are essential to detect apoptosis as one of mechanism in cell death.


Cite this article:
Lukisiari Agustini, Nurwasis, Aryati, Gatut Suhendro, Winarto, I Ketut Sudiana, Widjiati, Bambang Purwanto. Mechanism of Apoptosis Retinal Ganglion Cells Rattus norvegicus Caused by Ethambutol. Research Journal of Pharmacy and Technology. 2022; 15(4):1795-9. doi: 10.52711/0974-360X.2022.00301

Cite(Electronic):
Lukisiari Agustini, Nurwasis, Aryati, Gatut Suhendro, Winarto, I Ketut Sudiana, Widjiati, Bambang Purwanto. Mechanism of Apoptosis Retinal Ganglion Cells Rattus norvegicus Caused by Ethambutol. Research Journal of Pharmacy and Technology. 2022; 15(4):1795-9. doi: 10.52711/0974-360X.2022.00301   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-4-67


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