Author(s): Suyash Ingle, Varsha Tegeli, Avinash Birajdar, Gajanand Nangare

Email(s): suyashingle18@gmail.com

DOI: 10.52711/0974-360X.2022.00300   

Address: Suyash Ingle*, Varsha Tegeli, Avinash Birajdar, Gajanand Nangare
D.S.T.S. Mandal’s College of Pharmacy, Solapur - 413004, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 4,     Year - 2022


ABSTRACT:
The analytical method was developed and validated for determination of Alogliptin in bulk and pharmaceutical dosage forms by High performance liquid chromatography. The separation was carried out on Zorbax SB-Aq (250 x4.6mm,5µ ID) column. The mobile phase consists of 0.1% TFA Water : ACN in the ratio 62:38 at flow rate 1ml/min with diode array detector wavelength at 290nm.The column temperature was adjusted at 30º ± 0.5°C with injection volume 10µl.The retention time of Alogliptin was 3.06min. The linearity of the calibration curve was linear over the concentration range 25-75µg/ml (r2=1). The validation was carried out as per ICH guidelines. The development of method was easy, rapid, linear, precise, accurate and consistent.


Cite this article:
Suyash Ingle, Varsha Tegeli, Avinash Birajdar, Gajanand Nangare. Development and Validation of RP-HPLC Method for the Estimation of Alogliptin in API And Tablet Formulation. Research Journal of Pharmacy and Technology. 2022; 15(4):1791-4. doi: 10.52711/0974-360X.2022.00300

Cite(Electronic):
Suyash Ingle, Varsha Tegeli, Avinash Birajdar, Gajanand Nangare. Development and Validation of RP-HPLC Method for the Estimation of Alogliptin in API And Tablet Formulation. Research Journal of Pharmacy and Technology. 2022; 15(4):1791-4. doi: 10.52711/0974-360X.2022.00300   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-4-66


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