Author(s): A. Anka Rao, K. Pavan Kumar, A. Narayana Rao, Narender Malothu, B. Prassana Kumar Desu, Naga Raju Bandaru

Email(s): drankarao83@gmail.com

DOI: 10.52711/0974-360X.2022.00280   

Address: A. Anka Rao1*, K. Pavan Kumar2, A. Narayana Rao1, Narender Malothu1, B. Prassana Kumar Desu1, Naga Raju Bandaru1
1K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, India - 522 502.
2Research Scholar, K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, India-522 502.
*Corresponding Author

Published In:   Volume - 15,      Issue - 4,     Year - 2022


ABSTRACT:
Objective: Highly responsive simple Liquid-liquid Extraction Glipizide development using deuterated Glipizide and Sitagliptin LC-MS/MS Glipizide for gradation of Glipizide and Sitagliptin in rat plasma Glipizide: The chromatographic condition involves, isocratic mode using waters X-Bridge C18 3.5µ (150x4.6mm) column. Mobile phase 0.1% orthophosphoric acid and Acetonitrile in the ratio (80:20) was used and detection was carried out in a positive mode of electrospray ionization by using MS. Results: Glipizide has been validated, the linearity was observed in the range 10-150 ng/ml and 0.1-1.5 ng/ml for Glipizide and Sitagliptin respectively. For intraday and inter-day precision %RSD values were found to be within the acceptable limits. Recovery studies for Glipizide and Sitagliptin obtained was 99.5% and 98.6% respectively. The battery of stability studies like bench-top, Autosampler stability, freeze-thaw, and long term stability was performed. Conclusion: Highly responsive simple liquid chromatography tandem mass spectroscopy assay Glipizidewas developed and witnessed for the gradation of Glipizideand Sitagliptin in rat plasma, the developed Glipizidewas applied to pharmacokinetic studies.


Cite this article:
A. Anka Rao, K. Pavan Kumar, A. Narayana Rao, Narender Malothu, B. Prassana Kumar Desu, Naga Raju Bandaru. Rapid Quantitative Estimation of Glipizide and Sitagliptin in Rat Plasma by Liquid Chromatography and Mass spectroscopy (LC-MS). Research Journal of Pharmacy and Technology. 2022; 15(4):1675-9. doi: 10.52711/0974-360X.2022.00280

Cite(Electronic):
A. Anka Rao, K. Pavan Kumar, A. Narayana Rao, Narender Malothu, B. Prassana Kumar Desu, Naga Raju Bandaru. Rapid Quantitative Estimation of Glipizide and Sitagliptin in Rat Plasma by Liquid Chromatography and Mass spectroscopy (LC-MS). Research Journal of Pharmacy and Technology. 2022; 15(4):1675-9. doi: 10.52711/0974-360X.2022.00280   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-4-46


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