Baru Sadarun, Wahyuni, Muhammad Hajrul Malaka, Adryan Fristiohady, Agung Wibawa Mahatma Yodha, Nur Syifa Rahmatika, Zulfikri Saleh Islami, Muhammad Nurjayadin, Carla Wulandari Sabandar, Ahmad Darmawan, Andini Sundowo, Andi Rifky Rosandi, Sahidin I
Baru Sadarun1, Wahyuni2, Muhammad Hajrul Malaka2, Adryan Fristiohady2, Agung Wibawa Mahatma Yodha2, Nur Syifa Rahmatika3, Zulfikri Saleh Islami3, Muhammad Nurjayadin3, Carla Wulandari Sabandar4, Ahmad Darmawan5, Andini Sundowo5, Andi Rifky Rosandi6, Sahidin I2*
1Faculty of Fisheries and Marine Sciences, Universitas Halu Oleo, Kendari 93232, Indonesia.
2Faculty of Pharmacy, Universitas Halu Oleo, Kendari 93232, Indonesia.
3Faculty of Medicine, Universitas Halu Oleo, Kendari 93232, Indonesia.
4Department of Pharmacy, Faculty of Science and Technology, Universitas Sembilanbelas November Kolaka, Kolaka, Indonesia.
5Research Centre for Chemistry, National Research and Inovation Agency (BRIN), Kawasan Puspitek, Tangerang Selatan, Banten, Indonesia.
6School of Chemical Sciences and Food Technology, Universiti Kebangsaaan, Malaysia, 46000 Bangi, Selangor, Malaysia.
Volume - 15,
Issue - 4,
Year - 2022
Xestospongia sp. is one of marine sponge that can be found in Southeast Sulawesi. It belongs to Demospongiae classes which have shown many pharmacological activities such as antioxidant. Thus, this study aimed to identify isolates from Xestospongia sp. and its activity as antioxidant and anti-inflammatory. Isolation were carried out by chromatography technique including Thin Layer Chromatrography (TLC), vacuum liquid chromatography (VLC) and radial chromatography (RC) with silica gel as an adsorbent. Structure of isolated compounds were determined by spectroscopy methods i.e. FTIR, 1H and 13C NMR and also by comparison with those reported values. Biological activity of Xestospongia sp was also evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and Human Red Blood Cell (HRBC) methods. Four compounds isolated and identified from methanol extract of Xestospongia sp. were steroids that are (1) purchrasterol, (2) xestosterol, (3) saringosterol, and (4) 5a,8a-epidioxy-24a-ethylcholest-6-en-3ß-ol. The extract and the compounds showed antioxidant activity against DPPH radicals in which the extract was stronger than the isolated compounds. Furthermore, the Xestospongia sp. extract exhibited a dose-dependant anti-inflammatory activity by stabilizing red blood cell membranes at concentrations ranging from 50 to 3200 ppm. In conclusion, Xestospongia sp. extract which contain sterol compounds, such as purchrasterol, xestosterol, saringosterol and 5a,8a-epidioxy-24a-ethylcholest-6-en-3ß-ol provides antioxidant and anti-inflammatory activity.
Cite this article:
Baru Sadarun, Wahyuni, Muhammad Hajrul Malaka, Adryan Fristiohady, Agung Wibawa Mahatma Yodha, Nur Syifa Rahmatika, Zulfikri Saleh Islami, Muhammad Nurjayadin, Carla Wulandari Sabandar, Ahmad Darmawan, Andini Sundowo, Andi Rifky Rosandi, Sahidin I. Biological activities of Steroids and Extracts from Xestospongia sp. growing in Southeast Sulawesi (Indonesia). Research Journal of Pharmacy and Technology. 2022; 15(4):1487-3. doi: 10.52711/0974-360X.2022.00247
Baru Sadarun, Wahyuni, Muhammad Hajrul Malaka, Adryan Fristiohady, Agung Wibawa Mahatma Yodha, Nur Syifa Rahmatika, Zulfikri Saleh Islami, Muhammad Nurjayadin, Carla Wulandari Sabandar, Ahmad Darmawan, Andini Sundowo, Andi Rifky Rosandi, Sahidin I. Biological activities of Steroids and Extracts from Xestospongia sp. growing in Southeast Sulawesi (Indonesia). Research Journal of Pharmacy and Technology. 2022; 15(4):1487-3. doi: 10.52711/0974-360X.2022.00247 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-4-13
1. Resen AK, Alfekaiki DF, Othman NR. The Chemical Composition of Some Marine Fishes from the Iraqi Marine Waters. Research J. Science and Tech. 2017; 9(2), 231-233.
2. Sangeetha1 J, Gayathri1 S, Rajeshkumar S. Antimicrobial assessment of marine brown algae Sargassum whitti against UTI pathogens and its phytochemical analysis, Research J. Pharm. and Tech. 2017;10(6), 1905-1910.
3. Jenifer P, Balakrishnan CP, Chidambaram SP. In-vitro Antioxidant activity of Marine Red Algae Gracilaria foliifera. Asian J. Pharm. Tech. 2017; 7(2), 105-108.
4. Murugesan S, Bhuvaneswari S, Mahadeva Rao USM, Sivamurugan V. Screening of Phytochemicals and Antibacterial Activity of Marine Red Alga Portieria hornemannii (Lyngbye) P. C. Silva, Research Journal of Pharmacology and Pharmacodynamics. 2017; 9(3): 131-136.
5. Thirumurugan D and Vijayakumar R. Exploitation of Antibacterial Compound Producing Marine Actinobacteria against Fish Pathogens Isolated from Less Explored Environments. Research J. Science and Tech. 2013; 5(2), 264-267.
6. Manikandan R and Vijayakumar R. Screening of Antifouling Compound Producing Marine Actinobacteria against Biofouling Bacteria Isolated from Poultries of Namakkal District, South India. Research J. Science and Tech. 2016; 8(2), 83-89.
7. Chelvan Y, Chelvan T, Pushpam AC, Karthik R, Ramalingam K, Vanitha MC. Extraction and Purification of Antimicrobial Compounds from Marine Actinobacteria. Research J. Pharm. and Tech. 2016; 9(4),381-385.
8. Maheswari P, Mahendran S, Sankaralingam S, Sivakumar N. In vitro Antioxidant activity of Exoploysaccharide extracted from Marine Sediment Soil Bacteria. Research J. Pharm. and Tech. 2019; 12(9), 4496-4502.
9. Kanchana S and Arumugam M. Alternative exploration of hyaluronic acid from marine superstore. Asian J. Pharm. Res. 2014; 4 (4), 169-173.
10. Gopi K. and Jayaprakashvel M. Bioactive Potential of Marine Endophytic Fungi associated with Plants in Marine Ecosystem. Research J. Pharm. and Tech. 2017; 10(10), 3635-3636.
11. Sudayasa IP, Fristiohady A,Wahyuni, Sadarun B, Bafadal M, Purnama LOMJ, Rahmatika NS, Kalimin WOIL, Sahidin. Effect of Extract Marine Sponge Xestospongia sp. and Aaptos sp. on the Plasma IL-1β Level in Inflammatory Model Rats: Time Dependent. Research J. Pharm. and Tech. 2020; 13(11), 1-4.
12. Tan KG, Amri M, Ahmad NB and Merdikawati N. 2015 Annual Competitiveness Analysis And Development Strategies For Indonesian Provinces. World Scientific Publishing Co. Ptc. LTD. 2016
13. Longeoan A, Copp BR, Roue M, Dubois J, Valentin A, Petek S, Debitus C and Bourguet-Kondracki M. New bioactive helenaquinone derivatives from South Pacific marine sponges of the genus Xestospongia. Bioorganic & Medicinal Chemistry, 2010; 18(16), 6006-6011
14. Gauvin A, Smadja J, Aknin M, and Gaydou E.-M. Sterols composition and chemotaxonomic consideration in relation to sponges of the genus Xestospongia. Biochemical Systematic and Ecology. 2004; 32(5), 469-476
15. Sun L-L, Shao C-L, Chen J-F, Guo Z-Y, Fu X-M, Chen M, Chen Y-Y, Li R, de Voogd NJ, She Z-G, Lin Y-C, and Wang C-Y. New bisabolane sesquiterpenoids from a marine-derived fungus Aspergillus sp. isolated from the sponge Xestospongia testudinaria. Bioorganic & Medicinal Chemistry Letter. 2012, 22(3): 1326-1329.
16. He W-F, Li Y, Feng M-T, Gavagnin M, Mollo E, Mao S.-C, and Guo Y.-W. New Isoquinolinequinone Alkaloids From the South China Sea Nudibranch Jorunna Funebris and Its Possible Sponge-Prey Xestospongia Sp. Fitoterapia. 2014; 96, 109–114
17. Yang M, Liang LF, Yao LG, Liu HL, and Guo YW. A new brominated polyacetylene from Chinese marine sponge Xestospongia testudinaria. Journal of Asian Natural Products Research. 2019, 21(6):573-578
18. Millan-Aguinaga N, Sorea-Mercado IE, and Williams P. Xestosaprol D and E from the Indonesian marine sponge Xestospongia sp. Tetrahedron Letter, 2010, 51(4): 751-753.
19. Calcul L, Longeon A, Mourabit A, Guyot M, and Bourguet-Kondracki M. Novel alkaloids of aaptamine class from an Indonesian marine sponge of the genus Xestospongia sp. tetrahedron. 2003, 59(34): 6539-6544
20. Cita YP, Suhermanto A, Rajasa OK, and Sudharmono P. Antibacterial activity of marine bacteria isolated from sponge Xestospongia testudinaria from Sorong, Papua. Asia Pacific Journal of Tropical Medicine, 2017; 7(5), 450-454.
21. Murtihapsari, Salam S, Kurnia D, Darwati, Kadarusman, Abdullah FF, Herlina T, Husna MH, Awang K, Shiono Y, Azmi MN, Supratman U. A new antiplasmodial sterol from Indonesian marine sponge, Xestospongia sp., Natural Product Research. 2019; 1-8
22. Fristiohady A, Wahyuni, W, Kalimin WOIL, Purnama LOMJ, Saripuddin S, and Sahidin I. Anti-Inflammatory Activity of Marine Sponge Aaptos sp. to the Plasma Interleukin-1β Level in Wistar Male Rats. Pharmacology and Clinical Pharmacy Research, 2019; 4(2), 35–39
23. Fristiohady A, Wahyuni W, Malik F, Purnama LOMJ, Sadarun B, and Sahidin I. Anti-Inflammatory Activity Of Marine Sponge Callyspongia Sp. And Its Acute Toxicity. Asian Journal of Pharmaceutical and Clinical Research, 2019; 12(12), 97–100
24. Wahyuni W, Fristiohady A, Malaka MH, Malik F, Yusuf MI, Leorita M, Sadarun B, Saleh A, Musnina WOS, Sabandar CW and Sahidin I. Effects of Indonesian marine sponges ethanol extracts on the lipid profile of hyperlipidemic rats. Journal of Applied Pharmaceutical Science, 2019; 9(10), 001–008
25. Diaz P, Jeong SC, Lee S, Khoo C and Koyyalamudi SR. Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chin Med. 2012; 7(26), 1-9
26. Katzung B and Trevor A. Basic & Clinical Pharmacology (13th ed.). Mc. GrawHill. 2014
27. Sahidin I, Sabandar CW, Wahyuni, Hamsidi R, Mardikasari SA, Zubaydah WOS, Sadarun B, Musnina WOS, Darmawan A, and Sundowo A. Investigation of Compounds and Biological Activity of Selected Indonesian Marine Sponges. The Natural Products Journal, 2020; 10(3), 312–321.
28. Chippada SC, Volluri SS, Bammidi SR, and Meena V. In Vitro Anti Inflammatory Activity Of Methanolic Extract Of Centella Asiatica By Hrbc Membrane Stabilisation. Rasayan J Chem, 2011; 4(2), 457–460.
29. Pham N, Butler M, Hooper J, Moni R, and Quinn R. Isolation of Xestosterol Esters of Brominated Acetylenic Fatty Acids From the Marine Sponge Xestospongia Testudinaria. J Nat Prod ., 1999; 62(10), 1439–1442
30. Crist BV, Li X, Bergquist PR, and Djerassi C. Sterols of marine invertebrates. 44. Isolation, structure elucidation, partial synthesis, and determination of absolute configuration of pulchrasterol. The first example of double bioalkylation of the sterol side chain at position 26. J. Org. Chem, 1983; 48(24), 4472–4479
31. Gauvin A, Smadja J, Aknin M, Faure R, and Gaydou E.-M. Isolation of bioactive 5α,8α-epidioxy sterols from the marine sponge Luffariella cf. variabilis. Canadian Journal of Chemistry, 2000; 78(7), 986–992.
32. Zhou X, Lu Y, Lin X, Yang B, Yang X, and Liu Y. Brominated Aliphatic Hydrocarbons and Sterols From the Sponge Xestospongia Testudinaria With Their Bioactivities. Chem Phys Lipids, 2011; 164(7), 703–706.
33. El-Shitany NA, Shaala LA, Abbas AT, Abdel-Dayem UA, Azhar EI, Ali SS, van Soest RWM, and Youssef DTA. Evaluation of the Anti-Inflammatory, Antioxidant and Immunomodulatory Effects of the Organic Extract of the Red Sea Marine Sponge Xestospongia Testudinaria Against Carrageenan Induced Rat Paw Inflammation. PLoS ONE, 2015; 10(9), e0138917
34. Ericson-Neilsen W, and Kaye AD. Steroids: Pharmacology, Complications, and Practice Delivery Issues. Ochsner J, 2014; 14(2), 203–207.