The green resources which are utilized for the synthesis of nano particles are eco-friendly and helps to evade the practice of elevated use of harmful chemicals. Bio synthesis of silver nanoparticles (AgNPs) using Catharanthus roseus leaves was carried out with their characterization including UV spectroscopy, FTIR and SEM analysis. Qualitative phytochemical analysis was done to observe the presence of phyto compounds. The antioxidant assay was done to assess DPPH and Reducing power activities. The in vitro antidiabetic assay (a-Amylase Inhibition Assay) and the in-silico molecular docking were performed to analyze the antidiabetic potential of Catharanthus leaves. Target proteins such as 11ß-hydroxysteroid dehydrogenase type I (11ß-HSD1; PDB ID: 1XU7), Glucagon like peptide-1 (GLP-1; PDB ID: 3IOL), Protein-tyrosine phosphatase 1B (PTP1B; PDB ID: 4Y14) were chosen for molecular docking against the ligands screened from GCMS data. The colour change, UV-vis spectrum, FTIR and SEM examination supported the characterization of AgNPs. The synthesized nano particles showed a strong in vitro antioxidant activity with good scavenging percentage of 90.88% compared to the standard which was 99.84% in DPPH assay. Similarly, the inhibition of a-Amylase activity increased with increase in concentration of biosynthesized nano particles. The docking study revealed that plant compounds present have the highest binding affinity and good hydrogen bond interactions with active site residues. Hence the activities demonstrated suggest that they could be useful in the preparation of many therapeutic agents.
Cite this article:
Deepika E, Santhy KS. In vitro Antioxidant and Antidiabetic activity of Silver Nanoparticles Synthesized using Catharanthus roseus leaves. Research Journal of Pharmacy and Technology. 2022; 15(3):989-7. doi: 10.52711/0974-360X.2022.00165
Deepika E, Santhy KS. In vitro Antioxidant and Antidiabetic activity of Silver Nanoparticles Synthesized using Catharanthus roseus leaves. Research Journal of Pharmacy and Technology. 2022; 15(3):989-7. doi: 10.52711/0974-360X.2022.00165 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-3-8
1. American Diabetes Association. Standards of medical care in diabetes—2015 abridged for primary care providers. Clinical diabetes: a publication of the American Diabetes Association. 2015 Apr; 33(2): 97.
2. Patil R, Patil R, Ahirwar B, Ahirwar D. Isolation and characterization of anti-diabetic component (bioactivity—guided fractionation) from Ocimum sanctum L. (Lamiaceae) aerial part. Asian Pacific journal of tropical medicine. 2011;4(4): 278-82.
3. Sudha P, Zinjarde SS, Bhargava SY, Kumar AR. Potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Complementary and Alternative Medicine. 2011 ; 11(1):5.
4. Sales PM, Souza PM, Simeoni LA, Magalhães PO, Silveira D. α-Amylase inhibitors: a review of raw material and isolated compounds from plant source. Journal of Pharmacy & Pharmaceutical Sciences. 2012; 15(1):141-83.
5. Tripathi BK, Srivastava AK. Diabetes mellitus: complications and therapeutics. Medical Science Monitor. 2006; 12(7):130-47.
6. Jadhav GB, Saudagar RB. Free radical Scavenging and Antioxidant Activity of Punica granatum Linn. Asian Journal of Research in Pharmaceutical Science. 2014; 4(2):51-4.
7. Satish K, Vivek K and Chandrashekhar M.S. In-vitro anti-oxidant and alphaamylase inhibitory activity of isolated fractions from methanolic extract of Asystasia dalzelliana leaves. International Journal of Pharm Tech Research. 2011;3 (2): 889-894
8. Ahmad A, Senapati S, Khan MI, Kumar R, Ramani R, Srinivas V, Sastry M. Intracellular synthesis of gold nanoparticles by a novel alkalotolerant actinomycete, Rhodococcus species. Nanotechnology. 2003 Jun 6;14(7): 824.
9. Daniel MC, Astruc D. Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chemical Reviews. 2004; 104(1): 293-346.
10. Das S, Sharangi AB. Madagascar periwinkle (Catharanthus roseus L.): Diverse medicinal and therapeutic benefits to humankind. Journal of Pharmacognosy and Phytochemistry. 2017; 6(5): 1695-701.
11. Thilagam E, Parimaladevi B, Kumarappan C, Mandal SC. α-Glucosidase and α-amylase inhibitory activity of Senna surattensis. Journal of Acupuncture and Meridian Studies. 2013; 6(1): 24-30.
12. Mensor LL, Menezes FS, Leitão GG, Reis AS, Santos TCD, Coube CS and Leitão SG. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research. 2001; 15(2): 127-130.
13. Oyaizu M. Studies on product of browning reaction prepared from glucosamine. Japanese Journal of Nutrition and Dietetics.1986; (44): 307-15.
14. Nikavar B and Yousefian N. Inhibitory effects of six Allium species on α-amylase enzyme activity. 2009: 53-57.
15. Lipinski CA, Lombardo F, Dominy BW, Feeney PJ. Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Advanced Drug Delivery Reviews. 1997; 23(1-3):3-25.
16. DeLano WL. Pymol: An open-source molecular graphics tool. CCP4 Newsletter on protein crystallography. 2002; 40(1):82-92.
17. Maestro. Schrodinger. https://www.schrodinger.com/maestro
18. Friesner RA, Murphy RB, Repasky MP, Frye LL, Greenwood JR, Halgren TA, Sanschagrin PC, Mainz DT. Extra precision glide: Docking and scoring incorporating a model of hydrophobic enclosure for protein− ligand complexes. Journal of Medicinal Chemistry. 2006; 49(21):6177-96.
19. Velraj M, Shiney P, Paul B, Rashmi S. Biosynthesis of Silver Nano Particles from the Ethanolic Extract Fruits of Mallotus phillipensis. Research Journal of Pharmacy and Technology. 2017; 10(1):21-5.
20. Anima N, Shahnaz M, Mohd S, Nayak BK, Rizvi EH. Efficacy of nanosilver from soil fungus enhancing the antiseptic activity of Ciprofloxacin against pathogenic bacteria. Der Pharma Chemica. 2015; 7(6):141-6.
21. Devaraj P, Kumari P, Aarti C, Renganathan A. Synthesis and characterization of silver nanoparticles using Cannonball leaves and their cytotoxic activity against MCF-7 cell line. Journal of Nanotechnology. 2013.
22. Ahmed S, Saifullah, Ahmad M, Swami B, SaiqaIkram. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. Journal of Radiation Research and Applied Sciences. 2016; (9):1-7.
23. Mohamed NH, Ismail MA, Abdel-Mageed WM, Shoreit AA. Antimicrobial activity of latex silver nanoparticles using Calotropis procera. Asian Pacific Journal of Tropical Biomedicine. 2014; 4(11):876-83.
24. Vinay CH, Goudanavar P, Acharya A, Ahmed MG, Kumar PS. Development and characterization of orange peel extract-based nanoparticles. Asian Journal of Pharmaceutical Research. 2018;8(2):71-7.
25. Govindappa M, Hemashekhar B, Arthikala MK, Rai VR, Ramachandra YL. Characterization, antibacterial, antioxidant, antidiabetic, anti-inflammatory and antityrosinase activity of green synthesized silver nanoparticles using Calophyllum tomentosum leaves extract. Results in Physics. 2018; 9:400-8.
26. Ding R, Zhang H, Li Y, Wang J, Shi B, Mao H, Dang J, Liu J. Graphene oxide-embedded nanocomposite membrane for solvent resistant nanofiltration with enhanced rejection ability. Chemical Engineering Science. 2015; 138:227-38.
27. Priya AM, Selvan RK, Senthilkumar B, Satheeshkumar MK, Sanjeeviraja C. Synthesis and characterization of CdWO4 nanocrystals. Ceramics International. 2011;37(7):2485-8.
28. Hossain S, Hossain M, Haque Z, Uddin MP. Phytochemical screening of Catharanthus roseus and Ficus racemosa leaves extracts: A statistical inference. International Journal of Bioassays. 2015; 1:3606-10.
29. Govindasamy C, Srinivasan R. In vitro antibacterial activity and phytochemical analysis of Catharanthus roseus (Linn.) G. Don. Asian Pacific Journal of Tropical Biomedicine. 2012; 2(1):155-8.
30. Al-Shmgani HS, Mohammed WH, Sulaiman GM, Saadoon AH. Biosynthesis of silver nanoparticles from Catharanthus roseus leaf extract and assessing their antioxidant, antimicrobial, and wound-healing activities. Artificial Cells, Nanomedicine, and Biotechnology. 2017;45(6):1234-40.
31. Subhashini N, Thangathirupathi A, Lavanya N. Antioxidant activity of Trigonella foenum graecum using various in vitro and ex vivo models. International Journal of Pharmacy and Pharmaceutical Sciences. 2011;3(2):96-102.
32. Archana D, Dixitha M, Santhy KS. Antioxidant and anti clastogenic potential of Piper longum L. International Journal of Applied Pharmaceutics. 2015;7(2):11-4.
33. Tiwari P, Patel RK. Estimation of total phenolics and flavonoids and antioxidant potential of Ashwagandharishta prepared by traditional and modern methods. Asian Journal of Pharmaceutical Analysis. 2013;3(4):147-52.
34. Helen PA, Bency BJ. Inhibitory potential of Amaranthus viridis on α-amylase and glucose entrapment efficacy In vitro. Research Journal of Pharmacy and Technology. 2019;12(5):2089-92.
35. Bhusnure OG, Jadhav PP, Hindole SS, Gholve SB, Giram PS, Kuthar VS. Green Synthesis of Silver Nanoparticle Using Catharanthus roseus Extract for Pharmacological Activity. International Journal of Pharmacy and Pharmaceutical Research. 2017;10(4): 77-88.
36. Vega-Ávila E, Cano-Velasco JL, Alarcón-Aguilar FJ, Fajardo Ortíz MD, Almanza-Pérez JC, Román-Ramos R. Hypoglycemic activity of aqueous extracts from Catharanthus roseus. Evidence-Based Complementary and Alternative Medicine. 2012.
37. Govers R. Cellular regulation of glucose uptake by glucose transporter GLUT4. Advances in Clinical Chemistry. 2014; 66:173-240.
38. Al-Shaqha WM, Khan M, Salam N, Azzi A, Chaudhary AA. Anti-diabetic potential of Catharanthus roseus Linn. and its effect on the glucose transport gene (GLUT-2 and GLUT-4) in streptozotocin induced diabetic wistar rats. BMC Complementary and Alternative Medicine. 2015;15(1):1-8.
39. Kim MH, Jo SH, Jang HD, Lee MS, Kwon YI. Antioxidant activity and α-glucosidase inhibitory potential of onion (Allium cepa L.) extracts. Food Science and Biotechnology. 2010;19(1):159-64.
40. Pai V, Savadi RV, Bhandarkar A. In-Vitro Alpha-Amylase Inhibition Action of Isolated Phytoconstituent in Zanthoxylum rhetsa (Roxb) Bark. Research Journal of Pharmacy and Technology. 2011;4(7):1147-50.
41. Kumar KK, Sharma RS, Babu PC, Rao MS, Prasadu KD, Kumar DR. Synthesis, characterization and pharmacological evaluation of novel spiro heterocyclic compounds as anti diabetic agents. Asian Journal of Research in Chemistry. 2017;10(3):393-8.
42. Rahmani Z, Douadi A, Rahmani, Z. In vitro inhibition of a-amylase enzyme, phytochemical study and antioxidant capacity for Cupressus sempervirens extracts growing in arid climate. Asian Journal of Research in Chemistry. 2019;12(6):359-365.
43. Kumar PP, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. African Journal of Biochemistry Research. 2010;4(7):191-5.
44. Hamidi N, Lazuoni HA, Moussaouie A, Ziane L, Amal S. GC-MS Analysis of ethanol extract from the aerial parts of Fagonia longispina (family Zygophyllaceae). Asian Journal of Natural and Science. 2012; 1:136-42.
45. Lee YS, Kang MH, Cho SY, Jeong CS. Effects of constituents of Amomum xanthioides on gastritis in rats and on growth of gastric cancer cells. Archives of Pharmacal Research. 2007;30(4):436-43.
46. Thanwar M, Dwivedi D, Gharia AK. GC-MS study of methanolic extract of leaves of Catharanthus roseus. International Journal of Chemical Studies. 2017;5(2):517-8.
47. Malik A, Malik N, Dhiman P, Khatkar A, Kakkar S. Molecular docking, synthesis, α-amylase inhibition, urease inhibition and antioxidant evaluation of 4-hydroxy-3-methoxy benzoic acid derivatives. Research Journal of Pharmacy and Technology. 2019;12(12):5653-63.
48. Hameed SA, Varkey J, Jayasekhar P. Schiff bases and Bicyclic derivatives comprising 1, 3, 4-thiadiazole moiety-A Review on their Pharmacological activities. Asian Journal of Pharmaceutical Research. 2019;9(4):299-306.
49. Sensi M, Pricci F, De Rossi MG, Morano S, Di Marlo U. D-lysine effectively decreases the non-enzymic glycation of proteins in vitro. Clinical Chemistry. 1989;35(3):384-7.
50. Mehta V, Sharma A, Tanwar S, Malairaman U. In vitro and in silico evaluation of the antidiabetic effect of hydroalcoholic leaf extract of Centella asiatica. International Journal of Pharmacy and Pharmaceutical Sciences. 2016;8(8):357-62
51. Sivanandan S, Das A and Pimple S. Molecular Docking Studies of Alpinia galanga Phytoconstituents for Psychostimulant Activity. Advances in Biological Chemistry. 2018; 8:69-80.