Author(s): Samar El-Sayed Mahmoud, Abeer M. El-Shafey, A.T. Keshta


DOI: 10.52711/0974-360X.2022.00189   

Address: Samar El-Sayed Mahmoud1*, Abeer M. El-Shafey2, A.T. Keshta1
1Biochemistry Division, Chemistry Department, Faculty of Science, Zagazig University.
2Rheumatology and Rehabilitation Department, Faculty of Medicine, Zagazig University.
*Corresponding Author

Published In:   Volume - 15,      Issue - 3,     Year - 2022

Background and objective: Rheumatoid arthritis (RA) is a systemic autoimmune multifactorial complicated disease that is related to widespread morbidity and mortality. The current study aimed to study the relation between vitamin D receptor (VDR) gene polymorphism rs731236 (TaqI) and rs7975232 (ApaI) and RA susceptibility. Subjects, Materials and Methods: This study included 50 individuals were divided into two groups: Healthy volunteers group (10) and RA group (40) from Rheumatology and Rehabilitation Department, Faculty of Medicine, Zagazig university hospitals. DNA was extracted from whole blood for all samples, and PCR was amplified using specific primers rs 731236 (TaqI) and rs 7975232 (ApaI) VDR restriction digestion was carried out for TaqI and ApaI genotyping, respectively. Results: There were non-significant difference in VDR gene polymorphism between RA patients & control subjects. Conclusion: TaqI is more specific than ApaI but Taq1 and Apa1 gene polymorphism not used as a diagnostic marker for RA disease, so there is no relation between VDR and RA.

Cite this article:
Samar El-Sayed Mahmoud, Abeer M. El-Shafey, A.T. Keshta. VDR Gene Polymorphism and Rheumatoid Arthritis in Egyptian patients. Research Journal of Pharmacy and Technology. 2022; 15(3):1128-2. doi: 10.52711/0974-360X.2022.00189

Samar El-Sayed Mahmoud, Abeer M. El-Shafey, A.T. Keshta. VDR Gene Polymorphism and Rheumatoid Arthritis in Egyptian patients. Research Journal of Pharmacy and Technology. 2022; 15(3):1128-2. doi: 10.52711/0974-360X.2022.00189   Available on:

1.    Goldman L. Schafer A. eds. Rheumatoid arthritis. In: Goldman-Cecil Medicine. 2020; 2704.
2.    Burmester GR. Dimitriu-Bona A. Waters SJ. Winchester RJ. Identification of three major synovial lining cell populations by monoclonal antibodies directed to Ia antigens and antigens associated with monocytes/macrophages and fibroblasts. Scand. J. Immunol.1983; 17(1): 69–82.doi: 10.1111/j.1365-3083.1983.tb00767.X
3.    Magalhaes R. Stiehl P. Morawietz L. Berek C. Krenn V. Morphological and molecular pathology of the B cell response in synovitis of rheumatoid arthritis. Virchows Arch. 2002; 411(5)
4.    Singh S. Tiwari S. Tripathi S. Kumar S. A Review-Herbal Cure for Rheumatoid Arthritis. Research Journal of Pharmacognosy and Pytochemistry. 2016; 8(1).doi:10.5958/0975-4385.2016.00008.X
5.    Gary S Firestein. Evolving concepts of rheumatoid arthritis, Nature. 2003; 423(637): 356-361.
6.    Wolfe F. The natural history of rheumatoid arthritis. J. Rheumatoid Suppl. 1996; 44:13-22.
7.    Srivastava S. Patel S. Daharwal SJ. Singh D. Singh. Rheumatoid Arthritis: An Autoimmune Disease Prevalent in Femals. Research Journal of Pharmacy and Technology. 2016; 9(2). doi:10.5958/0974-360x.2016.00030.5
8.    Raychaudhuri S. Recent advances in the genetics of rheumatoid arthritis,. Current Opinion in Rheumatology. 2010; 22(2):109–118.doi:10.1097/BOR.0b013e328336474d
9.    Premkuma B. Antioxidant Defence and Disease activity in Rheumatoid Arthritis. Research Journal of Pharmacy and Technology. 2018; 11(5):0974-360X. doi:10.5958/0974-360x.2018.00336.0
10.     Moore C. Murphy MM. Keast DR. Hoilck MF. Vitamin D Intake in the UNITED Status. J Am Diet Assoc 2004; 104(6):980-983.doi:10.1016/j.jada.2004.03.028
11.    Shwetha MN. Chandra PPN. Knowledge regarding vitamin D deficiency among students. Asian Journal of Nursing Education and Research. 2019; 9(1).doi:10.5958/2349-2996.2019.00013.2
12.    Kiran G. Debashish D. Vitamin D and rheumatoid arthritis: is there a link? Int J Rheum Dis. 2008; 11(3): 206–
13.     Subashree R. Radhika A. Vitamin D Deficiency in Periodontal Health. Research Journal of Pharmacy and Technology. 2014; 7(2): 0974-360X.
14.    Amanzholkyzy A. Nurgaliyeva RE. Kaldybayeva AT. Balmaganbetova FK. Aibassova ZA. Biochemical variability of Viamin D Receptor (vdr) Gene and its Relationship with Bone Mineral Denisty of the Western Region of the Republic of Kazakhstan. Research Journal of Pharmacy and Technology. 2019; 12(2).doi:10.5958/0974-360X2019.00130.6
15.    Mukhtar M. Sheikh N. Suqaina SK. Batool A. Fatima N. Mehmood R. Nazir S. Vitamin D Receptor Gene Polymorphism: An Important Predictor of Arthritis Development. BioMed Research International. 2019; (8)
16.    Sabbagh Z. Markland J. Vatanparast H. Vitamin D status is associated with disease activity among rheumatology outpatients. Nutrients. 2013; 5(7):2268-75.doi:10.3390/nu5072268
17.    Kilic S. Silan F. Hiz MM. Isik S. Ogretmen Z. Odemir O. Vitamin D receptor gene BsmI, FokI, ApaI, and TaqI polymorphisms and the risk of atopic dermatitis. 2016; 26(2):106-110.doi:10.18176/jiaci.0020
18.    Jerome M. Lemaire C. Etienne M direct sequencing method for species identification of canned sardiene and sardine-type products. Journal of Agriculture and Food Chemistry. 2003; 51(25)
19.    Sheebanancy K. Maheswari C. Kavitha VJ. Vitamin D receptor polymorphism of ApaI (rs7975232) and TaqI (rs731236) in south indian postmenopausal osteoporotic and healthy women. 2017; 8(01)
20.    Levesque R. SPSS Programming and Data Management: A Guide for SPSS and SAS Users, Fourth Edition, SPSS Inc., Chicago, IL.2007; 60606-6412.
21.    Vishwabhan S. Harsh P. Vishal S. Kailash S. Some Traditionally Used Anti-Arthritis Herbs A Review. International Research Journal of Pharmacy. 2011; 2(11): 43-45.
22.    Cameron M. Gagnier JJ. Little CV. Parsons TJ. Blumle A. Chrubasik S. Evidence of Effectiveness of Herbal Medicinal Products in the Treatment of Arthritis; part 2: Rheumatoid Arthritis. Phytotherapy Research. 2009; 23(11)
23.     Kaur A. Nain P. Nain J. Herbal Plants Used in the Treatment of Rheumatoid Arthritis: A Review. International Journal of Pharmaceutical Sciences. 2012; 4(4): 44-57.
24.     Lee EB. Fleischmann R. Hall S. Wilkinson B. John D. Bradley, David Gruben, Tamas Koncz, Sriram Krishnaswami, Gene V. Wallenstien, Chuanbo Zang, Samuel H. Zwillich, and Ronald F. Van Vollenhoven, for the Oral Start Investigatoers. Tofacitinib versus Methotrexate in Rheumatoid Arthritis. The New England Journal of Medicine. 2014; 370: 2377-2386. doi:10.1056/NEJMoa131.476
25.    Perricone C. Ceccarelli F. Valesini G. An overview on the genetic of rheumatoid arthritis: a never-ending story. Autoimmun Rev. 2011; 10(10):599–608.doi:10.1016/j.autrev.2011.04.021
26.    Mac Gregor AJ. Snieder H. Rigby AS. Characterizing the quantitative genetic contribution to rheumatoid arthritis using data from twins. Arthritis Rheum. 2000; 43(1)<30::AID-ANR5>3.0.CO;2-B
27.    Rajalakshimi V. Vijey Aanandhi M. Hypovitaminosis D Influencec Chronic Ailments; Implication for Health. Research Journal of Pharmacy and Technology. 2018; 11(6): 0974-3618.
28.    Alam N. Agrawal OP. Rimpi. Alam P. Agrawal S. Kaushik M. Dhari JS. Sharma OP. Natural Immunoenhancers. Research Journal of Pharmacy and Technology. 2011; 10(4): 0974-3618.
29.    Ferrari S. Manen D. Bonjour JP. Slosman D. Rizzoli R. Bone Mineral Mass and Calcium and Phosphate Metabolism in Young Men: Relationships with Vitamin D Receptor Allelic Polymorphisms. Journal of Clinical Endocrinoligy & Metabolim. 1999; 84(6): 2043–2048. Doi:10.1210/jcem.84.6.5790
30.    Norman AW. Bouillon R. Vitamin D nutritional policy needs avisin for the future.; 235(9):1034-1045.doi:10.1258/ebm.2010.010014
31.    Dattola A. Silvestri M. Bennardo L. Passante M. Scali E. Patruno C. Nisticٍ SP. Role of Vitamins in Skin Health: A Systematic Review. Curr. Nutr. Rep. 2020; 9(3):226–235.doi:10.1007/s13668-020-00322-4
32.    Bragazzi, N.L.; Watad, A.; Neumann, S.G.; Simon, M.; Brown, S.B.; Abu Much, A.; Harari, A.; Tiosano, S.; Amital, H.; Shoenfeld, Y. Vitamin D and rheumatoid arthritis: An ongoing mystery. Curr. Opin. Rheumatol. 2017; 29(4): 378–388.doi:10.1097/BOR.0000000000000397
33.    Agmon-Levin, N.; Theodor, E.; Segal, R.M.; Shoenfeld, Y. Vitamin D in systemic and organ-specific autoimmune diseases. Clin. Rev. Allergy Immunol. 2013; 45(2): 256–266.doi:10.1007/s12016-012-8342-y
34.    Tizaoui K. Kaabachi W. Salah MO. AmorA AB. Hamzaoui. Hamzaoui K. Vitamin D receptor TaqI and ApaI polymorphisms: a comparative study in patients with Behcet’s disease and Rheumatoid arthritis in Tunisian population. Cellular Immunology. 2014; 290(1): 66–71.doi:10.1016/j.cellimm.2014.05.002
35.    Maalej A. Petit-Teixeira E. Michou L. Rebai A. Cornelis F. Ayadi H. Association study of VDR gene with rheumatoid arthritis in the French Population: 2005; 6(8): 707–711.
36.    Ateş O. Dölek B. Dalyan L. Topal-Sarikaya A. Vitamin D Receptor Gene Polymorphisms in Rheumatoid Arthritis: Turk J Rheumatol. 2011; 26(2):145-149.
37.    Wang W. Ailing Wu. Zhou Y. Wang Y. Cao K. Association between Vitamin D Receptor polymorphisms and rheumatoid arthritis risk: a meta-analysis: Int J Clin Exp Med. 2017; 10(2):4221-4233.
38.    Atoum M. Alzoughool F. Vitamin D deficiency and rs731236 (Taq1) vitamin D receptor gene polymorphism as possible risk factors for rheumatoid arthritis and osteoarthritis: Acta Medica Mediterranea. 2018; 34: 209.

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