K. Sruthi, B. Anupama, N.L. Sudeepthi, P. Gopi Krishna, Abdul Kareem, Mohammad Habeeb
K. Sruthi1*, B. Anupama2, N.L. Sudeepthi2, P. Gopi Krishna2, Abdul Kareem2, Mohammad Habeeb3
1Department of Pharmaceutical Chemistry, Vijaya Institute of Pharmaceutical Sciences for Women, Vijayawada, Andhra Pradesh, India.
2Department of Pharmaceutical Chemistry, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India.
3Department of Pharmaceutics, Crescent School of Pharmacy, B.S. Abdur Rahman Crescent Institute of Science and Technology, Vandalur, Chennai, Tamilnadu, India.
Volume - 15,
Issue - 2,
Year - 2022
There are many drugs for alopecia such as minoxidil, glucocorticoids, sulfasalazine, nitric oxide, and vitamin D3. Hair loss is also known as alopecia, or baldness commonly refers to hair loss from either a portion of a head or body. Fresh Leaves, and bark from twigs of Wrightia tinctoria were taken and used for extraction. A phytochemical assessment was continued to carry out on W.tinctoria extract and the presence of aromatic hydrogen and aromatic protons was demonstrated by spectral characterization. Insilco studies were done. Compounds 1and6 shown more docking values than the minoxidil and Swiss ADME confirmed that the compounds can enter the blood-brain barrier and not the substrates of PgP carriers. In vivo evaluation of alopecia activity using albino male, Wistar rats were done by comparing it with standard drug involves the evaluation of in vivo hair development activities of W.tinctoria extract. It also involves histopathological studies which include the action of hair growth at anagen and telogen phases on 15th and 30th day. The study indicates that the material elements of W.tinctoria which include Quercetin and Indirubin playing a critical role in hair promoting activity.
Cite this article:
K. Sruthi, B. Anupama, N.L. Sudeepthi, P. Gopi Krishna, Abdul Kareem, Mohammad Habeeb. Investigation of Wrightia tinctoria extract activity on Alopecia using In-silco and In-vivo studies. Research Journal of Pharmacy and Technology. 2022; 15(2):643-9. doi: 10.52711/0974-360X.2022.00106
K. Sruthi, B. Anupama, N.L. Sudeepthi, P. Gopi Krishna, Abdul Kareem, Mohammad Habeeb. Investigation of Wrightia tinctoria extract activity on Alopecia using In-silco and In-vivo studies. Research Journal of Pharmacy and Technology. 2022; 15(2):643-9. doi: 10.52711/0974-360X.2022.00106 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-2-26
1. Amruthesh S. Dentistry and Ayurveda-III (basics-ama, immunity, ojas, rasas, etiopathogenesis and prevention). Indian J Dent Res. 2007; 18(3):112.
2. Anusharaj, Chandrashekar, Prabhakar Adake, SN Rao, Santanusaha. Wrightia Tinctoria: An Overview. J. Drug DelivTher. 2013; 3(2):196-98.
3. Ashish Dixit, A K Jain, Pawan Tiwari, Nidhi Gupta, Priyanka Gangele. A Phyto pharmacological review on an important medicinal plant – Wrightia tinctoria. Curr. Res. Pharm. Sci. 2014; 04(03):70-76.
4. Battu Ganga Rao, Dodda L. N. Rajeswari, Devarakonda Ramadevi and Battu Heera. Phytochemical and pharmacological studies on Wrightia tinctoria. World J Pharm Pharm Sci. 2018; 7(4):562-8.
5. Dandagi PM, Pandey P, Gadad AP, Mastiholimath VS. Formulation and Evaluation of Micro emulsion based Luliconazole Gel for Topical Delivery. Indian J. Pharm. Educ. Res. 2020; 54(2):293-301.
6. Daniel M, Sabnis SD. A chemotaxonomic appraisal of the status of Apocynaceae and Asclepiadaceae.Curr. Med. Chem. 1982; 1(2): 84-90.
7. Dhanabal SP, Baskar Anand Raj, Muruganantham N, Praveen TK, Raghu PS. Screening of Wrightia tinctoria leaves for Anti psoriatic activity. Hygeia J. D. Med, 2012: 4(1),73-78.
8. Basavaraj S Adi, Geeta B Adi and Siva Rami Reddy E. Wrightia tinctoria: A review. Int. J. Hom. Sci. 2019; 3(2): 10- 13.
9. Evan Darwin, Penelope A Hirt, Raymond Fertig, Brett Doliner, Gina Delcanto and Joaquin J Jimenez. Alopecia areata : Review of epidemiology, clinical features, pathogenesis and new treatment options. Int J Trichology.2018; 10(2):51-60.
10. Frederich M, Tits M and Angenot L. Potential antimalarial activity of indole alkaloids. Trans R Soc Trop Med Hyg. 2008; 10(2):11-19.
11. Gade VKV, Mony A., Munisamy M, Chandrashekar L, Rajappa M. An investigation of Vitamin D3 in alopecia areata. Clin Exp Med. 2018; 18(4):577-84.
12. Ghosh D, Thejmoorth P, Veluchamy G. Anti-inflammatory, analgesic and antipyretic activities of777 oil-a siddha medicine. Bull Med Ethno Botany and Research.1985;6(2-4):141-54.
13. Jain PS and Bari SB. Preliminary phytochemical screening of woody stem extracts of A belmsochusmani hot and Wrightia tinctoria. Int. Pharma Sci. 2011; 1(3):59-63.
14. Jain PS, Bari SB. Antibacterial and antifungal activity of extracts of woody stem of Wrightia tinctoria R. Br. Int. J. Pharm. Sci. Res. 2009; 1:18-21.
15. Jolly C L. and Mercher. N R. Comparative pharmacognostical, physicochemical and antibacterial studies on seeds of Holarrhenaantidysenterica wall and Wrightia tinctoria R. Br. Indian J Pharm Sci. 1994; 58(2):51-4.
16. Kannan P, Petchiammal C, Mohankumar R and Hopper W. In vitro antifungal activity of indirubin isolated from a south Indian ethno medicinal plant Wrightia tinctoria R. Br. J Ethnopharmacol. 2010; 132:349-54.
17. Khyade MS, Vaikos NP. Pharmacognostical and physio-chemical standardization of leaves of Wrightia tinctoria R. Br. Indian J Nat Prod Resour. 2011; 2(4): 464-471.
18. Khyade MS, Vaikos NP. Wrightia tinctoria R. Br. A review on its ethno botany, Pharmacognosy’ and pharmacological profile. J. Coast Life Med. 2014; 2(10):826-40.
19. Kothari MJ, Rao KM. Ethno botanical studies of Thane District Maharashtra,” In, JK Maheshwari Ed.: Ethno botany and Medicinal plants of Indian Subcontinent. Scientific Publisher. 2000; 4:78-9.
20. Kumaran RS, Muthumary J, Hur BK. Isolation and identification of an anticancer drug, taxol from Phyllostictatabernaemontanae, a leaf spot fungus of an angiosperm Wrightia tinctoria. J. Appl. Microbiol. 2009; 47(1):40
21. Leona Yip, Nick Rufaut, Rod Sinclair. Role of genetics and sex steroid hormones in alopecia areata. Australas. J. Dermatol. 2011; 52(2):81-8.
22. M. Sandhya Rani, Rao S. Pippalla, G. Krishna Mohan, M. Gangaraju. Anti- diabetic activity of methanolic and ethyl acetate extracts of Wrightia tinctoria R.BR. Fruit. Int. J. Pharm. Sci. Res. 2012; 3(10):3861-66.
23. McElwee KJ, Gilhar A, Tobin DJ, Ramot Y. What causes alopecia areata? EXP Dermatol. 2013; 22(9): 609-26.
24. Melbi Babu and Hareeshbabu E. Pharmacological Evaluation of Wrightia tinctoria: A Review. Int. J. Pharm. Med. Sci. 2018; 1(4): 73-74.
25. Nagariya AK, Meena AK, Jain D, Gupta BP, Yadav AK et al. Medicinal plants used in the healing of skin diseases in different regions of India: a review. Int.J.Chem. Anal Sci. 2010; 1(5):110-13.
26. P. Selvam, N. Murugesh, M. Witvrouw, E. Keyaerts, J. Neyts. Study of anti-viral and cytotoxicity of Wrightia tinctoria. Indian J Pharm Sci. 2009; 71(6):670-72.
27. Parvathi A, Kumari DS. Chemo taxonomical studies of some Apocynaceae and Asclepiadaceae. Indian J Bot. 1984; 11(5): 607-28.
28. Ponnusamy K, Petchiammal C, Mohankumar R, Hopper W. In-vitro antifungal activity of indirubin isolated from south Indian ethno medical plant Wrightia tinctoria. J. Ethnopharmacol. 2010; 132(1): 349-54.
29. R Suraja, G Rejitha. A, J Anbu Jeba Sunilsonab, K Anandarajagopala, P Promwichit. In-vivo hair growth activity of Prunusdulcis seeds in rats. Free Radic. Biol. Med. 2009; 1(4):34-8.
30. Ramalakshmi S, Edaydulla. N, Ramesh. P, Muthuchelian. K. Investigation on cytotoxic, antioxidant, antimicrobial and volatile profile of Wrightia tinctoria (Roxb.) R. Br. Flower used in Indian medicine. Asian Pac J Trop Dis. 2012; 2(10):68-75.
31. Rashidi T, Mahd AA. Treatment of persistent alopecia areata with sulfasalazine. Int. J. Dermatol. 2008; 47(8):850-2.
32. Sharma VK. Pulsed administration of corticoids in the treatment of alopecia areata. Int. J. Dermatol.1996; 35(3):133-6.
33. Sravanthi Maddila and K.P.J. Hemalatha. Phytochemical Screening and Invitro Antimicrobial Properties of Crude Leaf Extracts of Wrightia tinctoria R. Br. Int J Curr Microbiol Appl Sci. 2017; 6(1):707-20.
34. Srivastava R. A review on phytochemical, pharmacological, and pharmacognostical profile of Wrightia tinctoria: Adulterant of kurchi. Pharmacognosy Reviews. 2014; 8(15):36–44.
35. T. C. Wikramanayake. S. Amini, J. Simon, L. M. Mauro. A novel rat model for chemotherapy-induced alopecia. Clin. Exp. Dermatol. 2012; 37(3):284-89.
36. Tare HL, Gore MS, Deore SR and Bidkar JS. Comparative hemintholytic potential of extracts obtained from Cymbopogoncitratus and Wrightia tinctoria leaves. Int. J. Pharma and Bio-Sci. 2011; 2(1):321-27.
37. Thas JJ. Siddha medicine background and principles and the application for skin diseases. Clin Dermatol. 2008; 26(1):62-78.
38. Tomar R, Kumar R, Jagannadham. A stable serine protease, Wrightia, from the latex of the plant Wrightia tinctoria (Roxb.) R. Br.: Purification and Biochemical Properties. J. Agric. Food Chem. 2008; 56(4):1479-87.
39. Weiss VC, West DP, Fu TS, Robinson LA, Cook B, Cohen RL, Chambers DA. Arch Dermatol.1984; 120(4):457-63.
40. Cassiana Mendes, Lilian Klein, Maximiliano da Silva Sangoi, Larissa Sakis Bernardi, and Marcos Antonio Segatto Silva. Formulation Development and Stability Studies of Norfloxacin Extended-Release Matrix Tablets. Bio med Research International. 2013; 4: 716-736.