Author(s): Noviana Dwi Lestari, Wahyu Isnia Adharini, Widodo, Sri Rahayu, Hideo Tsuboi, Yoga Dwi Jatmiko, Muhaimin Rifa’i

Email(s): immunobiology@ub.ac.id

DOI: 10.52711/0974-360X.2022.00103   

Address: Noviana Dwi Lestari1, Wahyu Isnia Adharini1, Widodo1, Sri Rahayu1, Hideo Tsuboi2, Yoga Dwi Jatmiko1, Muhaimin Rifa’i1*
1Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java, Indonesia.
2Department of Immunology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
*Corresponding Author

Published In:   Volume - 15,      Issue - 2,     Year - 2022


ABSTRACT:
This study aimed to evaluate the effect of Moringa-Albumin (MA) combination on pro-inflammatory cytokine expressions, especially IFN-? and TNF-a, in a diabetic mouse model. Streptozotocin with a 145 mg/kg BW dose was used to induce diabetes condition in BALB/c mice. Mice with positive DM (blood glucose levels = 200 mg/dL) were orally administered with MA for 14 days at dose 1, dose 2, and dose 3. On day 15th, spleen cells were isolated to analyze IFN-? and TNF-a expressions by flow cytometry. The data were statistically analyzed with one-way ANOVA (?= 0.05) and Tukey test using SPSS version 16 for Windows. The results showed that the MA combination had anti-inflammatory activity in inhibiting IFN-? and TNF-a. Furthermore, dose 1 affected to decrease in the IFN-? expression while dose 3 decreased the expression of TNF-a. Thus, it can be concluded that the MA combination has a role in inhibiting IFN-? and TNF-a in a dosage-dependent manner. Based on the results, we assumed that MA might be one of the biological materials with efficacy to treat DM patients.


Cite this article:
Noviana Dwi Lestari, Wahyu Isnia Adharini, Widodo, Sri Rahayu, Hideo Tsuboi, Yoga Dwi Jatmiko, Muhaimin Rifa’i. Anti-inflammatory Evaluation of Moringa-Albumin Combination in Inhibiting IFN-γ and TNF-α Expression in Diabetic Mouse Model. Research Journal of Pharmacy and Technology. 2022; 15(2):628-2. doi: 10.52711/0974-360X.2022.00103

Cite(Electronic):
Noviana Dwi Lestari, Wahyu Isnia Adharini, Widodo, Sri Rahayu, Hideo Tsuboi, Yoga Dwi Jatmiko, Muhaimin Rifa’i. Anti-inflammatory Evaluation of Moringa-Albumin Combination in Inhibiting IFN-γ and TNF-α Expression in Diabetic Mouse Model. Research Journal of Pharmacy and Technology. 2022; 15(2):628-2. doi: 10.52711/0974-360X.2022.00103   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-2-23


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