Author(s): Maram Jbaily, Firas Hussein, Nisrin Kaddar

Email(s): maramgbily@gmail.com

DOI: 10.52711/0974-360X.2022.00913   

Address: Maram Jbaily1, Firas Hussein2, Nisrin Kaddar3
1Postgraduate Student, Department of Biochemistry and Microbiology, Faculty of Pharmacy, Tishreen University, Lattakia, Syria.
2Assistant Professor, Department of Internal Medicine and Heamatology, Faculty of Medicine, Tishreen University, Lattakia, Syria.
3Professor, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Tishreen University, Lattakia, Syria.
*Corresponding Author

Published In:   Volume - 15,      Issue - 12,     Year - 2022


ABSTRACT:
Background: Macrocytic anemia is common and is characterized by decreased hemoglobin levels with elevated Mean Corpuscular Volume (MCV), due to a range of diseases and divided into Megaloblastic and Non-megaloblastic anemia. Serum vitamin B12 and folic acid tests are usually performed but they are limited by their low sensitivity and specificity. To confirm diagnosis of macrocytic anemia bone marrow examination is required but it is invasive procedure. Vitamin B12 and/or B9 deficiency leads to a defect in DNA synthesis leading to ineffective erythropoiesis and intramedullary hemolysis in patients of megaloblastic anemia, this leads to increased serum LDH (Lactate Dehydrogenase) and unconjugated bilirubin. Aims of study: This study was carried out to evaluate the role of serum LDH in the distinction between megaloblastic and non-megaloblastic anemia, and to study the correlation between serum LDH and MCV Patient and Methods: The study included 60 patients with non-regenerative macrocytic anemia (We exclude patients with regenerative macrocytic anemia because elevated reticulocytes leads us to hymolysis anemia or bleeding and it’s not a diagnostic problem(. Complete blood count, biochemical investigation, peripheral blood examination, reticulocyte count, bone marrow examination was performed in all cases Results: The most common cause of macrocytic anemia was Megaloblastic anemia (65%). The other causes were primary bone marrow disorders (35%). There was a significant difference in the mean values of serum LDH and MCV between two groups ( megaloblastic and non-megaloblastic anemia). When LDH>2076.5 IU/L, there is more probability of having megaloblastic anemia than non-megaloblastic anemia but when LDH>2076.5 and MCV>109.45 with bilirubin> 1.2mg/dl will must more probably not have non-megaloblastic anemia (the specificity was 100%), all three parameters combined together can be used as screening test to distinguish between the 2 groups of macrocytic anemia (megaloblastic and non-megaloblastic) without necessity of bone marrow aspiration in patients of non- regenerative macrocytic anemia. Present study had also shown that there were a positive relationship between serum LDH and MCV (r=+0.613).


Cite this article:
Maram Jbaily, Firas Hussein, Nisrin Kaddar. The Role of Serum Lactate Dehydrogenase in Etiological Diagnosis of Macrocytic Anemia. Research Journal of Pharmacy and Technology2022; 15(12):5421-4. doi: 10.52711/0974-360X.2022.00913

Cite(Electronic):
Maram Jbaily, Firas Hussein, Nisrin Kaddar. The Role of Serum Lactate Dehydrogenase in Etiological Diagnosis of Macrocytic Anemia. Research Journal of Pharmacy and Technology2022; 15(12):5421-4. doi: 10.52711/0974-360X.2022.00913   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-12-7


REFERENCES:
1.    Rajan T.S, Yeswanthi S. A Prospective Study on Quality of Life in Menorrhagia Patients with Anemia. Research Journal of Pharmacy Technology. 2017 10(7): p. 2233-2235. Doi: 10.5958/0974-360X.2017.00395.X
2.    Garg S.K, et al. Prevalence of Anemia among Antenatal mothers attending antenatal OPD's in selected hospitals of Punjab. International Journal of Nursing Education. 2020 8(2): p. 175-178. Doi: 10.5958/2454-2660.2020.00039.3
3.    Kumar V, et al.  A Study to assess knowledge about Anemia among the women (18-45years) at rural area of Mysore District. Asian Journal of Nursing Education Research. 2020 10(4): p. 471-473. Doi: 10.5958/2349-2996.2020.00100.7
4.    Seppä K,  et al.  Evaluation of macrocytosis by general practitioners. J Stud Alcohol. 1996 Jan 57(1): p. 97-100. Doi: 10.15288/jsa.1996.57.97.
5.    Aslinia F, et al. Megaloblastic anemia and other causes of macrocytosis. Clinical Medicine & Research. 2006 Sep 4(3): p. 236-241.Doi: 10.3121/cmr.4.3.236.
6.    Nadar S, Vijaykumar M. Prevalence of anemia in urban children attending a pediatric hospital of a metro city in South India. Research journal of Pharmacy Technology. 2016 9(10): p. 1571-1574. Doi: 10.5958/0974-360X.2016.00308.5
7.    Colon-Otero G, et al.  A practical approach to the differential diagnosis and evaluation of the adult patient with macrocytic anemia. Med Clin North Am. 1992 May 76(3): p. 581-97. Doi: 10.1016/s0025-7125(16)30341-8.
8.    Castellanos-Sinco H, et al. Megaloblastic anaemia: Folic acid and vitamin B12 metabolism.Revista Médica Del Hospital General De México. 2015 Sep 78(3): p .135-143.  Doi:10.1016/j.hgmx.2015.07.001.
9.    Nagao T, Hirokawa M. Diagnosis and treatment of macrocytic anemias in adults. J Gen Fam Med. 2017 Apr. 18(5): p. 200-204.Doi: 10.1002/jgf2.31.
10.    Nasser R, Hussein F. Corelation between Pretreatment Serum LDH levels and Therapeutic response in Cancer patients. Research Journal of Pharmacy Technology, 2020 13(3): p. 1107-1112. Doi: 10.5958/0974-360X.2020.00203.6
11.    Salameh H, Hussein F. Diagnostic value of serum LDH in pre-Treatment Cancer Patients. Research Journal of Pharmacy Technology. 2020 13(3): p. 1303-1308. Doi: 10.5958/0974-360X.2020.00240.1
12.    Shekar P, et al. Analysis of Lymphoma Neoplasm and their Phenotypes. Research Journal of Engineering Technology. 2013 4(4): p. 191-194.
13.    Nandakumar E, Savitha G. A study of Salivary Lactate Dehydragenase (LDH) level in normal individuals and the Oral Cancer Patients. Research Journal of Pharmacy Technology. 2015 8(7): p. 932-934 Doi: 10.5958/0974-360X.2015.00155.9
14.    Gupta M, et al.  Serum lactate dehydrogenase in diagnosis of megaloblastic anaemia-An observational study in Central India. Journal of Medical Science and Clinical Research. 2019 Jul. 7(7): p. 603-610. Doi: 10.18535/jmscr/v7i7.106.
15.    Kaferle J, Strzoda CE. Evaluation of macrocytosis. American family physician 2009 Feb. 79(3): p. 203-208.
16.    Patidar D, et al. A Study to assess the effectiveness of Planned teaching Programme on knowledge regarding Vitamin B12 deficiency among the people in selected Areas of Mehsana City. International Journal of Nursing Education Research. 2021 9(1): p. 88-90. Doi: 10.5958/2454-2660.2021.00022.3
17.    Haddad N.AI, Abdulrahman N.A. Correlation of Vitamin B12 deficiency with H. pylori infection and other Biochemical parameters in Iraqi patients. Research Journal of Pharmacy Technology. 2020 13(11): P. 5451-5454. Doi: 10.5958/0974-360X.2020.00951.8
18.    Khanduri U, Sharma A. Megaloblastic anaemia: prevalence and   causative factors. The National    Medical Journal of India 2007.  20(4): p. 172-175.
19.    Unnikrishnan V, et al. Clinico-aetiologic profile of macrocytic anemias with special reference to megaloblastic anemia. Indian Journal of Hematology Blood Transfusion, 2008 Dec. 24(4): p. 155-165.Doi: 10.1007/s12288-008-0039-2.
20.    Chakravarty N, et al. Study of serum lactate dehydrogenase level as diagnostic and prognostic indicator of megaloblastic anemia. International Journal of  Advances in Medicine, 2019 Aug. 6(4): p. 1199-1206. Doi: 10.18203/2349-3933.ijam20193271.
21.    Libnoch JA, et al. Lactate dehydrogenase in megaloblastic bone marrow. American  journal of clinical patholog. 1966 Mar. 45(3): p. 302-305. Doi: 10.1093/ajcp/45.3.302.
22.    Jaswal T, et al. Serum lactate dehydrogenase in diagnosis of megaloblastic anaemia. Indian journal of pathology microbiology. Indian journal of pathology microbiology. 2000 Jul. 43(3): p. 325-329
23.    Kannan  A, et al. Evaluation of clinical, biochemical and hematological parameters in macrocytic anemia. International Journal of Research in Medical Sciences. 2016 Jul. 4 (7): p. 2670-2678.Doi: 10.18203/2320-6012.
24.    Thimmappa N, et al. Evaluation of clinical, biochemical and hematological parameters in macrocytic anemia. International Journal of  Advances in Medicine. 2019 Apr. 6(2): p. 489-494. Doi: 10.18203/23493933.ijam20191164
25.    Chaudhari  S, Bindu S. Correlation of lactate dehydrogenase in megaloblastic anemia. . International Journal of Current Medical Applied Sciences. 2015 Dec. 9(1) p. 28-32.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available