Author(s): Gajender Singh, Dheeraj Bisht, Rajeshwar Kamal Kant Arya, Sunil Kumar, Deepak Kumar, Ashutosh K. Dash

Email(s): ashutosh.dash10@gmail.com , guptadeepak002@gmail.com

DOI: 10.52711/0974-360X.2022.00974   

Address: Gajender Singh1, Dheeraj Bisht2, Rajeshwar Kamal Kant Arya2, Sunil Kumar1, Deepak Kumar1*, Ashutosh K. Dash3*
1Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Shoolini University, Solan - 173229, India.
2Department of Pharmaceutical Sciences, Sir J. C. Bose Technical Campus Bhimtal, Kumaun University, Nainital - 263136, Uttarakhand, India.
3Devsynthesis India Pvt. Ltd, Drug Discovery Chemistry CRO, Hyderabad, India – 500076.
*Corresponding Author

Published In:   Volume - 15,      Issue - 12,     Year - 2022


ABSTRACT:
Picrorhiza kurroa (kutki) is a hairy herb belonging to Plantaginaceae/ Scropulariacea family and found in some parts of India (Kashmir to Sikkim), Nepal, and China.They found application in various ailments such as inflammation, antioxidant activity, asthmatic activity, hepatic activity, etc. The compound constituents using HPLC retrieved the presence of eighteen compounds. HPTLC analysis confirmed and quantified the presence of six major compounds. The quantity of diosgenin was found to be (2.80%) maximum followed by chlorogenic acid (2.70% w/w of extract) in the DCM/Methanol leaf extract. Antibacterial activity was performed using the zone of inhibition method for eight different leaf extracts of Picrorhiza kurroa. Among the extracts, ethyl acetate extract showed good potency in terms of zone of inhibition (13.33±1.52) against S. aureus whereas n-butanol extract showed good potency (12.01±1.00) against S. mutans, acetone extracts showed good potency (16.66±.47) against E. coli, acetone extracts showed good potency (11.33±1.52) against K. pneumoniae and acetone extracts showed good potency (11.00±1.00) against P. aeruginosa. Methanol extract showed good antioxidant activity (41.3±3.1µg/ml) whereas n-hexane showed the least (389.5±1.3µg/ml). Chlormphenicol and Ascorbic acid were taken as standard in the antibacterial and antioxidant study. IC50 values were calculated from the known protocol.


Cite this article:
Gajender Singh, Dheeraj Bisht, Rajeshwar Kamal Kant Arya, Sunil Kumar, Deepak Kumar, Ashutosh K. Dash. Identification and Quantification of six natural compounds from Picrorhiza kurroa leaf extract and their Antibacterial and Antioxidant activity. Research Journal of Pharmacy and Technology 2022; 15(12):5774-8. doi: 10.52711/0974-360X.2022.00974

Cite(Electronic):
Gajender Singh, Dheeraj Bisht, Rajeshwar Kamal Kant Arya, Sunil Kumar, Deepak Kumar, Ashutosh K. Dash. Identification and Quantification of six natural compounds from Picrorhiza kurroa leaf extract and their Antibacterial and Antioxidant activity. Research Journal of Pharmacy and Technology 2022; 15(12):5774-8. doi: 10.52711/0974-360X.2022.00974   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-12-68


REFERENCES:
1.    Sharma SK. Kumar N. Antimicrobial screening of Picrorhiza kurroa Royle Ex Benth rhizome. International Journal of Current Pharmaceutical Review and Research. 2012; 3(3): 60-65.
2.    Kumar N. Kumar T. Sharma SK. Phytopharmacological review on genus Picrorhiza. International Journal of Universal Pharmacy and Bio Sciences. 2013; 2(4): 334-346.
3.    Bhattacharjee S., Jana S., Baghel DS. A review on medicinally important species of Picrorhiza. International Journal of Pharmaceutical Research and Biosciences. 2013; 2(4): 1-16.
4.    Ali M., Sultana S., Mir SR. Chemical constituents from the roots of Picrorhizakurroa Royle Ex Benth. International Journal of Pharmacy and Pharmaceutical Research. 2017; 9(3): 25-35.
5.    Kant K., Wallia M,. Agnihotri VK., Pathania V., Singh B. Evaluation of antioxidant activity of Picrorhizakurroa (Leaves) extracts. Indian Journal of Pharmaceutical Sciences. 2013; 75(3): 324-329.doi: 10.4103/0250-474X.117438
6.    Bisht D., Kumar D., Kumar D., Dua K., Chellappan DK. Phytochemistry and pharmacological activity of the genus artemisia. Arch. Pharm. Res. 2021; 44: 439–474.  https://doi.org/10.1007/s12272-021-01328-4
7.    MadhviSK.SharmaM. IqbalJ. YounisM. Phytochemistry, Traditional uses and Pharmacology of Rhododendron arboreum: A Review. Research Journal of Pharmacy and Technology. 2019;12(9): 4565-4574.
8.    Shukla SS, Sharma V, Gidwani B, Vyas A, Daharwal SJ, Pandey RK. Chromatographic fingerprint: A modern scientific tool for standardization of traditional medicines. Research Journal of Pharmacy and Technology. 2021; 14(7): 4003-4010.
9.    AL-Taey, Duraid K, Abbas K, Mijwel, Saad S, Al-Azawy. Study efficiency of poultry litter and kinetin in reduced effects of saline water in Viciafaba. Research Journal of Pharmacy and Technology. 2018;11(1): 294-300.
10.    Panchale.Wrushali A. Rajnandini S. Minakshi S. Rathod. YL. Pandhare. Chromatographic analysis of famotidine, paracetamol and ibuprofen from tablet formulation. Research Journal of Pharmacy and Technology. 2019;12(1): 231-236.
11.    McCloud TG. High throughput extraction of plant, marine and fungal specimens for preservation of biologically active molecules. Molecules, 2010; 15:4526-4563.
12.    Singh G. Pathania R. Khan M.Tonk RK. Kumar D. DashAK. Identification and quantification of some natural compounds of Pinus gerardiana leaf extract and its antimicrobial and antioxidant activities.Pharmacologyonline. 2021; 2: 333-351.
13.    Aatral GV. Samraj S. Kavitha S. Arunkumar N. Nakkeeran E. Surendran G. Preparation of Anti-immunodeficiency pills by the Herbal solution. Research Journal of Pharmacy and Technology. 2021; 14(6):3244-3248.
14.    Mohanty S. Sharma M. Dubey K. Alexander A. Khan J. Khan S. Tripathi DK.Significance of Herbal Plants in the Treatment of Sickle Cell Anaemia. Research Journal of Pharmacy and Technology. 2012; 5(3): 297-301.
15.    Krishna L. Venkata S. Narenderan ST. Nayak R. Meyyanathan SN. Babu B. Kalaivani M. Development and Validation of An Analytical Liquid Chromatography-Tandem Mass Spectroscopy Method for the Estimation Febuxostat in Pharmaceutical Formulation. Research Journal of Pharmacy and Technology. 2019; 12(5): 2137-2140.
16.    MariA. David L. Lena F. Paola M. Sonia P. Stefanie W. et al Phytochemical composition of Potentilla anserina L. analyzed by an integrative GC-MS and LC-MS metabolomics platform, Metabolomics. 2013; 9: 599–607.
17.    Praveen P. Singh S. Dubey D. Dashora K. Estimation of Rutin in Tephrosiapurpurea by HPTLC Method. Research Journal of Pharmacy and Technology. 2013; 6(1): 58-60.
18.    Bhole RP. Shinde SS. Chitlange SS. Wankhede SB.  A High-Performance Thin Layer Chromatography (HPTLC) Method for Simultaneous Determination of Diphenhydramine Hydrochloride and Naproxen Sodium in Tablets, Analytical Chemistry Insights. 2015; 10: 47-51.
19.    Xie PS.  A feasible strategy for applying chromatography fingerprint to assess quality of Chinese herbal medicine, Traditional Chinese Drug Research and Clinical Pharmacology. 2001; 12 (3): 141–169.
20.    Devi J. Pachwania S. Kumar D. Jindal DK. Suraya J. Dash AK. Diorganotin(IV) complexes derived from thiazole Schiff bases: synthesis, characterization, antimicrobial and cytotoxic studies. Research on Chemical Intermediate. 2021; 1-23.
21.    Husain A. Rashid M. Mishra R. Kumar D. Bis-chalcones and flavones: synthesis and antimicrobial activity. Acta Poloniae Pharmaceutica -Drug Research. 2013; 70 (3): 443-449.
22.    Devi J. Kumar S. Kumar D. Jindal DK. Poornachandra Y. Synthesis, characterization, in vitro antimicrobialand cytotoxic evaluation of Co(II), Ni(II), Cu(II) and Zn(II) complexes derived from bidentate hydrazones. Research on Chemical Intermediate. 2022; 423–455.
23.    Jagtap NS. Khadabadi SS. Ghorpade DS. Banarase NB. Naphade SS. Antimicrobialand antifungal activity of Centella asiatica (L.) Urban, Umbeliferae. Research Journal of Pharmacy and Technology. 2009; 2(2): 328-330.
24.    Vignesh S. Roy A. Rajesh KS. Lakshmi.T.  Evaluation of the antimicrobialactivity of cumin oil mediated silver nanoparticles on oral microbes. Research Journal of Pharmacy and Technology. 2019; 12(8): 3709-3712.
25.    KumarD. Kumar R. Ramajayam R. Lee KW. ShinD-S.Synthesis, antioxidant and molecular docking studies of (−)-catechin derivatives, Journal of the Korean Chemical Society. 2021; 65: 106-112.
26.    Kumar K. Kumar D. Jindal DK. Yadav M. Sharma N. GuptaR.. Comparative antioxidant activity of roots and fruits of Piper sylvaticum Roxb, J. Compr. Pharm. 2016; 3: 132- 135.
27.    Valarmathy J. Samueljoshua L.Rathinavel G. Thanuja CS. Kumar S.RP-HPLC Method development and validation for assay of Levetiracetam in tablet dosage Form. Research Journal of Pharmacy and Technology. 2008;1(4): 395-397.

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