Author(s): Appu Anoop, Thilagar Gobinath, Samuthirapandian Ravichandran


DOI: 10.52711/0974-360X.2022.00966   

Address: Appu Anoop1, Thilagar Gobinath1, Samuthirapandian Ravichandran2*
1Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
2Government Thirumagal Mills College, Gudiyatham.
*Corresponding Author

Published In:   Volume - 15,      Issue - 12,     Year - 2022

Chitosan is derived from Murex trapa as the starting source by the process deacetylation of chitin, which is carried out for 6 hours using 40% NaOH at 90ºC. The yield (17%) and physiochemical properties like ash (0.954%), moisture content (4.2%), and solubility, degree of deacetylation (73), fat binding capacity (252%), and water binding capacity (280%) were indicated the M. trapa is a substantial alternate source of chitosan. Fourier transforms infrared spectroscopy (FT-IR) analysis shows characteristic peaks of OH at 3450cm-1 and amine at 1660cm-1, X-ray diffraction (XRD) analysis indicated two vital characteristic peaks 10° and 20° at (2?). Scanning electron microscope (SEM) was used to determine surface morphology of isolated chitosan. Also, Thermogravimetric analysis (TG/DTA) was employed to characterize the thermal stability of M. trapa chitosan. Procoagulant ability, plasma recalcification time assays and minimum bactericidal activity confirmed the hemocompatibility and antibacterial activity of the prepared chitosan. The isolated chitosan can be considered as a potent anticoagulant and antibacterial drug in future.

Cite this article:
Appu Anoop, Thilagar Gobinath, Samuthirapandian Ravichandran. Physicochemical properties and Structural Characterization of Chitosan Synthesized from Rare Spined Murex, Murex trapa (Roding, 1798) Shell Waste. Research Journal of Pharmacy and Technology 2022; 15(12):5729-5. doi: 10.52711/0974-360X.2022.00966

Appu Anoop, Thilagar Gobinath, Samuthirapandian Ravichandran. Physicochemical properties and Structural Characterization of Chitosan Synthesized from Rare Spined Murex, Murex trapa (Roding, 1798) Shell Waste. Research Journal of Pharmacy and Technology 2022; 15(12):5729-5. doi: 10.52711/0974-360X.2022.00966   Available on:

1.    K. Mohan et al., “Extraction and characterization of chitin from sea snail Conus inscriptus (Reeve, 1843),” Int. J. Biol. Macromol., vol. 126, pp. 555–560, 2019, doi: 10.1016/j.ijbiomac.2018.12.241.
2.    Samira Boufas, Mohamed El Hocine Benhamza, Bachir Ben Seghir, Ferdenache Hadria. Synthesis and Characterization of Chitosan/Carrageenan/Hydroxyethyl cellulose blended gels. Asian J. Research Chem. 2020; 13(3):209-215. doi: 10.5958/0974-4150.2020.00040.1
3.    AVS Madhu Latha, T Naga Ravikiran, J N Suresh Kumar. Formulation, Optimization and Evaluation of Glibenclamide Transdermal Patches by using chitosan Polymer. Asian J. Pharm. Tech. 2019; 9(1):01-07.
4.    Anitha T, Sathishkumar K.. Comparative Study of Adsorption for Chitosan, Sand and Chitosan Coated Sand. Asian J. Pharm. Tech. 2013; Vol. 3: Issue 4, Pg 185-188.
5.    Kiran Patil, Vasant Helavi. Synthesis of Pyranopyrazoles by using Chitosan Hydrogel as a green and recyclable catalyst. Asian J. Research Chem. 2018; 11(2):477-484. doi: 10.5958/0974-4150.2018.00087.1
6.    H. Rasti, K. Parivar, J. Baharara, M. Iranshahi, and F. Namvar, “Chitin from the Mollusc Chiton: Extraction, Characterization and Chitosan Preparation.,” Iran. J. Pharm. Res.  IJPR, vol. 16, no. 1, pp. 366–379, 2017.
7.    Nikhil D. Shirsat, S. A. Momin, Ashish A. Bandekar, Usha Sayed. Synthesis of Water Soluble Chitosan from Marine Waste and Its Application in Wet Wipes Formulations. Asian J. Research Chem. 5(12): Dec., 2012; Page 1419-1423.
8.    Y. I. Cho, H. K. No, and S. P. Meyers, “Physicochemical Characteristics and Functional Properties of Various Commercial Chitin and Chitosan Products,” J. Agric. Food Chem., vol. 46, no. 9, pp. 3839–3843, 1998, doi: 10.1021/jf971047f.
9.    Z. Hu et al., “Investigation of the effects of molecular parameters on the hemostatic properties of chitosan,” Molecules, vol. 23, no. 12, pp. 1–14, 2018, doi: 10.3390/molecules23123147.
10.    H. Khalili, R. Soltani, S. Negahban, A. Abdollahi, and K. Gholami, “Reliability of disk diffusion test results for the antimicrobial susceptibility testing of nosocomial gram-positive microorganisms: Is e-test method better?,” Iran. J. Pharm. Res., vol. 11, no. 2, pp. 559–563, May 2012, doi: 10.22037/ijpr.2012.1094.
11.    S. O. Majekodunmi, E. O. Olorunsola, and C. C. Uzoaganobi, “Comparative Physicochemical Characterization of Chitosan from Shells of Two Bivalved Mollusks from Two Different Continents,” Am. J. Polym. Sci., vol. 7, no. 1, pp. 15–22, 2017, doi: 10.5923/j.ajps.20170701.03.
12.    P. Ramasamy, N. Subhapradha, V. Shanmugam, and A. Shanmugam, “Extraction, characterization and antioxidant property of chitosan from cuttlebone Sepia kobiensis (Hoyle 1885),” Int. J. Biol. Macromol., vol. 64, no. July 2019, pp. 202–212, 2014, doi: 10.1016/j.ijbiomac.2013.12.008.
13.    M. Syifa Yusharani, I. Ulfin, and Y. Lailun Ni, “Synthesis of water-soluble chitosan from squid pens waste as raw material for capsule shell: temperature deacetylation and reaction time Recent citations Doxorubicin-Loaded Squid Pen Plaster: A Natural Drug Delivery System for Cancer Cells Giulia Magnabos,” doi: 10.1088/1757-899X/509/1/012070.
14.    A. Singh, S. Benjakul, and T. Prodpran, “Ultrasound-Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity,” J. Food Sci., vol. 84, no. 2, pp. 224–234, Feb. 2019, doi: 10.1111/1750-3841.14439.
15.    I. Younes and M. Rinaudo, “Chitin and chitosan preparation from marine sources. Structure, properties and applications,” Marine Drugs, vol. 13, no. 3. MDPI AG, pp. 1133–1174, Mar. 2015, doi: 10.3390/md13031133.
16.    Nikhil D. Shirsat, S. A. Momin, Usha Sayed. Development of biodegradable polysaccharide by modified process from sea desecrates and its proximate analysis. Asian J. Research Chem. 5(8): August, 2012; Page 1078-1083.
17.    S. Kumari and P. K. Rath, “Extraction and Characterization of Chitin and Chitosan from (Labeo rohit) Fish Scales,” Procedia Mater. Sci., vol. 6, pp. 482–489, Jan. 2014, doi: 10.1016/j.mspro.2014.07.062.
18.    Sachin R. Kumbhoje, Zaki Husain J. Tamboli, Dr. John I. D’Souza. Synthesis and Characterization of Schiff bases of Chitosan for their Improved Mucoadhesion. Asian J. Research Chem. 5(9): September, 2012; Page 1099-1103.
19.    L. S. Guinesi and É. T. G. Cavalheiro, “The use of DSC curves to determine the acetylation degree of chitin/chitosan samples,” Thermochim. Acta, vol. 444, no. 2, pp. 128–133, May 2006, doi: 10.1016/j.tca.2006.03.003.
20.    Hassan T. Abdulsahib, Abdulamir H. Taobi, Salah Sh. Hashim. Removal of Bentonite from Raw water by Novel Coagulant Based on Chitosan and Tannin. Asian J. Research Chem 8(4): April 2015; Page 241-252. doi: 10.5958/0974-4150.2015.00042.5
21.    Jeba Jeevitha R.S, Bella G.R, S. Avila Thanga Booshan. Preparation and Characterization of Micro Crystalline Cellulose Fiber Reinforced Chitosan based Polymer Composites. Asian J. Research Chem. 8(7): July- 2015 ; Page 453-458. doi: 10.5958/0974-4150.2015.00074.7
22.    Z. Hu, D. Y. Zhang, S. T. Lu, P. W. Li, and S. D. Li, “Chitosan-based composite materials for prospective hemostatic applications,” Marine Drugs, vol. 16, no. 8. MDPI AG, Aug. 2018, doi: 10.3390/md16080273.
23.    L. W. Chan, C. H. Kim, X. Wang, S. H. Pun, N. J. White, and T. H. Kim, “PolySTAT-modified chitosan gauzes for improved hemostasis in external hemorrhage,” Acta Biomater., vol. 31, pp. 178–185, Feb. 2016, doi: 10.1016/j.actbio.2015.11.017.
24.    Monika Singh, Sangeeta Loonker. Microwave assisted synthesis of chitosan epoxy asparagine hydroxamate (CE-AH) Characterization and Study of its antimicrobial activity. Asian J. Research Chem. 2017; 10(4):497-503. doi: 10.5958/0974-4150.2017.00081.5
25.    A. Shanmugam, K. Kathiresan, and L. Nayak, “Preparation, characterization and antibacterial activity of chitosan and phosphorylated chitosan from cuttlebone of Sepia kobiensis (Hoyle, 1885),” Biotechnol. Reports, vol. 9, pp. 25–30, Mar. 2016, doi: 10.1016/j.btre.2015.10.007.

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