Sreekala. V, K. N. Dwivedi, Santosh Kumar Singh
Sreekala. V1*, K. N. Dwivedi2, Santosh Kumar Singh3
1Research Scholar (Senior Research Fellow - CCRAS), Dept. of Dravyaguna Vijnana, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
2Dean, Faculty of Ayurveda, Institute of Medical Science, Banaras Hindu University, Varanasi, India.
or Scientist, Centre for Experimental Medicine and Surgery, Banaras Hindu University, Varanasi, India.
Volume - 15,
Issue - 12,
Year - 2022
Background: Cocos nucifera Linn. belongs to the Arecaceae family and is the only accepted species from the genus Cocos. Coconut shell is a usually discarded material from the plant source Cocos nucifera Linn. However, scientifically validated data regarding the chemical and conforming therapeutic profile of Coconut shell is not available till date. It is important to develop analytical standards of Coconut shell using pharmacognostic, phytochemical and chromatographic techniques. Materials and Methods: The present analytical study was designed to determine the organic compounds present in the active fraction of dried ripe Coconut shell and to develop drug standards using powder microscopy, quantitative and qualitative phytochemical analysis and TLC techniques. Results: Powder microscopy and preliminary phytochemical analysis of Cocos nucifera Linn. endocarp indicated the presence of high fibre content in the drug. Further studies revealed the test drug as a rich source of alkaloids, steroids, phenols, flavonoids and tannins. Bioactive chemical profile and analytical standards of the test drug were derived by Thin Layer Chromatographic studies as well. Conclusion: The findings of the current research work ensued in establishing the botanical and analytical standards for Coconut (Cocos nucifera Linn.) shell.
Cite this article:
Sreekala. V, K. N. Dwivedi, Santosh Kumar Singh. Development and Validation of Analytical Standards of Coconut (Cocos nucifera Linn.) shell based on Pharmacognostic, Phytochemical and Chromatographic Techniques. Research Journal of Pharmacy and Technology 2022; 15(12):5677-1. doi: 10.52711/0974-360X.2022.00957
Sreekala. V, K. N. Dwivedi, Santosh Kumar Singh. Development and Validation of Analytical Standards of Coconut (Cocos nucifera Linn.) shell based on Pharmacognostic, Phytochemical and Chromatographic Techniques. Research Journal of Pharmacy and Technology 2022; 15(12):5677-1. doi: 10.52711/0974-360X.2022.00957 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-12-51
1. Altemimi A. Lakhssassi N. Baharlouei A. Watson DG. Lightfoot DA. Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants (Basel). 2017; 6 (4):42.doi:10.3390/plants6040042
2. Punam S. Desai. Rajeshwar V. Chavan. Nikita B. Amane. Sanmati D. Shete. Archana R. Dhole. High Performance Liquid Chromatography – A Validation View.Asian J. Pharm. Ana. 2019; 9(4):232-236.doi: 10.5958/2231-5675.2019.00040.1
3. Sasidharan S. Chen Y. Saravanan D. Sundram KM. Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plant extracts. Afr J Tradit Complement Altern Med. 2011; 8 (1):1-10. PMID: 22238476; PMCID: PMC3218439.
4. R. Suja Pandian, A. Thajun Noora. GC-MS Analysis of Phytochemical Compounds Present in the Leaves of Citrus medica. L. Research J. Pharm. and Tech. 2019; 12(4):1823-1826. doi: 10.5958/0974-360X.2019.00304.4
5. World Health Organization. (2004). WHO guidelines on safety monitoring of herbal medicines in pharmacovigilance systems. World Health Organization.
6. Suraj J. Patil. Shivani D. Patil. Pratibha B. Patil. Pranali S. Patil. Ganesh B. Vambhurkar. Indryani D. Raut. Evaluation of Standardization Parameters of Ayurvedic Marketed Polyherbal Formulation. Asian J. Pharm. Ana. 2018; 8(4): 220-226. doi: 10.5958/2231-5675.2018.00040.6
7. Shubham Jaiswal. Sarin A Chavhan. Sushilkumar A Shinde. N K Wawge. New Tools for Herbal Drug Standardization. Asian J. Res. Pharm. Sci. 2018; 8(3): 161-169. doi: 10.5958/2231-5659.2018.00029.2.
8. Ragavee A, Tenzin Choedar, Sourabh Suman, S. Asha Devi. Evaluation of Bioactive Constituents and Antioxidant Activity of Mucilage Isolated from Tinospora cordifolia. Research J. Pharm. and Tech 2018; 11(7): 2747-2751. doi: 10.5958/0974-360X.2018.00507.3
9. Steinhoff. Barbara. Review: Quality of herbal medicinal products: State of the art of purity assessment. Phytomedicine. International Journal of Phytotherapy and Phytopharmacology. 2019: vol. 60. 153003. doi: 10.1016/j.phymed.2019.153003.
10. Trease and Evans WC. Pharmacognosy. 16th ed. London.Saunders Elsevier.2009; 567-568.
11. Government of India. Ayurvedic Pharmacopoeia of India. New Delhi: Government of India Ministry of Health and Family Welfare Department of AYUSH. Part1, Volume 1 (2018).
12. M.E. Pascual, M.E. Carretero, K.V. Slowing and A. Villar (2002) Simplified Screening by TLC of Plant Drugs, Pharmaceutical Biology, 40:2, 139-143, doi: 10.1076/phbi.22.214.171.12449
13. Qingyue Wang. J Sarkar. Pyrolysis behaviours of waste Coconut shell and husk biomasses.International Journal of Energy Production and Management. 2018; 1(3):34-43. doi: 10.2495/EQ-V3-N1-34-43
14. Maria Pilar Vinardell. Montserrat Mitjans. Lignins and their derivatives with beneficial effects on human health. International Journal of Molecular Sciences. 2017; 18(6):12-19. doi: 10.3390/ijms18061219.
15. Rajesh R. Singh SA. Vaithy KA. Manimekalai K. Kotasthane D. Rajasekar SS. The effect of mucuna pruriens seed extract on pancreas and liver of diabetic wistar rats. Int J Cur Res Rev. 2016;8(4):61-70.
16. Bhavana Srivastava. Himanshu Sharma. Vikas Chandra Sharma. S.C. Verma. A.D. Jadhav. Pterocarpus marsupium, physicochemical analysis, phytochemical analysis, HPTLC profile. Res. J. Pharmacognosy and Phytochem. 2016; 8(2): 53-59. doi:10.5958/0975-4385.2016.00010.8
17. P. Selvakumar. Devi Kaniakumari. V. Loganathan. Physicochemical Analysis of Alocasia sanderiana W. Bull. Asian J. Pharm. Ana. 2016; 6(1): 31-34.
18. Vidya S. Kukde, Mrunali A. Bhongade, Sweety S. Kamde. Standardization and Phytochemical Screening of Ficus religiosa. Research J. Pharm. and Tech. 2021; 14(2):971-976. doi: 10.5958/0974-360X.2021.00173.6
19. Sreekala V. Vijaykishan B. Rajasekar S. Rajesh R. Phytochemical analysis and in vitro DPPH assay of ethanolic extract of Mucuna pruriens seed. International Journal of Current Pharmaceutical Research. 2020; 12(6):93-96. doi: http://dx.doi.org/10.22159/ijcpr.2020v12i6.40300
20. R Ramasubramaniaraja. Pharmacognostical Phytochemical Including GC-MS Investigation of Ethanolic Leaf Extracts of Abutilon indicum (Linn). Asian J. Pharm. Ana. 2011; 1(4):88-92.
21. V. Krishna Murthy Naik,. K. Sudhakar Babu. P Malleswara Reddy. J Latha. Evaluation of Phytocomponents from Ether Extract of Azdiractha indica by HPLC-Fame Method. Asian J. Research Chem. 2018; 11(2):400-404. doi: 10.5958/0974-4150.2018.00041.X