Author(s): Rajiniraja Muniyan, Sadhana Sundararajan, Johana Stalin Jochebed

Email(s): rajiniraja.m@vit.ac.in

DOI: 10.52711/0974-360X.2022.00946   

Address: Rajiniraja Muniyan*, Sadhana Sundararajan, Johana Stalin Jochebed
School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore - 632014, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 12,     Year - 2022


ABSTRACT:
This study aims to investigate the traditional value of home-based medicinal plants, their importance in improving the immune system and their antimicrobial/antimycobacterial potential. This is based on the fact that traditionally indigenous plants are used to treat ailments but the constituents of the plants are largely unexplored. The aqueous extracts of some medicinal plants based on their ease of availability were screened against 2 common strains of microorganisms namely Escherichia coli (E.coli) and Mycobacterium smegmatis (M. smegmatis) using plate assay. Aqueous extract of S.trilobatum L., with significant inhibition properties against E.coli and M. smegmatis was taken further to bioactivity guided extraction. The extraction was done using solvents of increasing polarity. Subsequently, the extract with maximum activity was subjected to GC-MS to explore the presence of phytochemical (s). On screening with the extracts, it was seen that, the Petroleum ether extract showed significant bactericidal activity with an inhibition zone of 12 and 16mm in diameter for E.coli and M.smegmatis respectively. In the current study, it is demonstrated that the extracts of S.trilobatum L possess antimycobacterial activity. Our data draws attention towards S.trilobatum as a promising source of active biomolecules that can be included in diet and supports the fact that it is used as a traditional medicine.


Cite this article:
Rajiniraja Muniyan, Sadhana Sundararajan, Johana Stalin Jochebed. Antimicrobial and Antimycobacterial properties of Solanum trilobatum L. - In-vitro. Research Journal of Pharmacy and Technology 2022; 15(12):5603.9 doi: 10.52711/0974-360X.2022.00946

Cite(Electronic):
Rajiniraja Muniyan, Sadhana Sundararajan, Johana Stalin Jochebed. Antimicrobial and Antimycobacterial properties of Solanum trilobatum L. - In-vitro. Research Journal of Pharmacy and Technology 2022; 15(12):5603.9 doi: 10.52711/0974-360X.2022.00946   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-12-40


REFERENCES:
1.    Etusim PE, Okafor EE, Nwachukwu NC, Melariri PE, Ogbonnaya CI. A Study on Antibacterial activities of Aloe vera Leaves, Stems and Roots on some selected organisms. Asian Journal of Research In Chemistry. 2013 Jun 1; 6(6):570-2.
2.    Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology. 2014 Jan 10; 4:177. doi.org/10.3389/fphar.2013.00177
3.    WHO report. (2009) doi:10.1017/CBO9781107415324.004.
4.    Bodeker GC, Bhat KK, Burley J, Vantomme P. Medicinal Plants for Forest Conservation and Health Care. FAO; 1997.
5.    Mital NM. Antibacterial Activity of Leaves and Flowers of Ipomoea aquatica Forsk.(Convolvulacea). Asian Journal of Pharmaceutical Research. 2018; 8(2). doi.org/10.5958/2231-5691.2018.00016.3
6.    Dahake AP, Joshi AB, Patel KM. Antimicrobial investigation of different extracts of Dolichos lablab beans. Research Journal of Pharmacognosy and Phytochemistry. 2009; 1(2):123-5.
7.    Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. 2017 Sep 22; 6(4):42.
8.    Brothers, H. Environmental Health Perspectives. Oxford Univ. XXX, 60 (1895).
9.    Rao N, Mittal S, Menghani E. Assessment of phytochemical screening, antioxidant and antibacterial potential of the methanolic extract of Ricinus communis L. Asian Journal of Pharmacy and Technology. 2013; 3(1):20-5.
10.    Santa Packyanathan J, Prakasam G. Antibacterial effect of clove oil against clinical strains of Escherichia coli. Journal of Pharmaceutical Sciences and Research. 2017 Jul 1; 9(7):1203.
11.    Kumar KS, Yadav A, Srivastava S, Paswan S, sankar Dutta A. Recent trends in Indian traditional herbs Syzygium aromaticum and its health benefits. Journal of Pharmacognosy and Phytochemistry. 2012; 1(1):13-22.
12.    Yap PS, Krishnan T, Chan KG, Lim SH. Antibacterial mode of action of Cinnamomum verum bark essential oil, alone and in combination with piperacillin, against a multi-drug-resistant Escherichia coli strain. Journal of Microbiology and Biotechnology. 2015; 25(8):1299-306.
13.    Qais FA, Khan MS, Althubiani AS, Al-Ghamdi SB, Ahmad I. Understanding biochemical and molecular mechanism of complications of glycation and its management by herbal medicine. InNew Look to Phytomedicine 2019 Jan 1 (pp. 331-366). Academic Press.doi:10.1016/B978-0-12-814619-4.00013-6.
14.    Sharif MK, Ejaz R, Pasha I. Nutritional and therapeutic potential of spices. InTherapeutic, probiotic, and unconventional foods 2018 Jan 1 (pp. 181-199). Academic Press.doi:10.1016/b978-0-12-814625-5.00011-x.
15.    Mathew S, Abraham TE. Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models. Food Chemistry. 2006 Mar 1; 94(4):520-8.
16.    De Falco E, Mancini E, Roscigno G, Mignola E, Taglialatela-Scafati O, Senatore F. Chemical composition and biological activity of essential oils of Origanum vulgare L. subsp. vulgare L. under different growth conditions. Molecules. 2013 Dec 4; 18(12):14948-60.
17.    Huda JA, Imad HH, Salah AI, Mohammed YH. Biochemical analysis of Origanum vulgare seeds by fourier-transform infrared (FT-IR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Journal of Pharmacognosy and Phytotherapy. 2015 Sep 30; 7(9):221-37.
18.    De Santis F, Poerio N, Gismondi A, Nanni V, Di Marco G, Nisini R, Thaller MC, Canini A, Fraziano M. Hydroalcoholic extract from Origanum vulgare induces a combined anti-mycobacterial and anti-inflammatory response in innate immune cells. PloS one. 2019 Mar 4; 14(3):e0213150.
19.    Singh R, Shushni MA, Belkheir A. Antibacterial and antioxidant activities of Mentha piperita L. Arabian Journal of Chemistry. 2015 May 1; 8(3):322-8.
20.    İşcan G, Ki̇ri̇mer N, Kürkcüoǧlu M, Başer HC, Demirci F. Antimicrobial screening of Mentha piperita essential oils. Journal of Agricultural and Food Chemistry. 2002 Jul 3; 50(14):3943-6.
21.    Shirwaikar A, Rajendran K, Bodla R, Kumar CD. Neutralization potential of Viper russelli russelli (Russell’s viper) venom by ethanol leaf extract of Acalypha indica. Journal of Ethnopharmacology. 2004 Oct 1; 94(2-3):267-73.
22.    Rahman MA, Bachar SC, Rahmatullah M. Analgesic and antiinflammatory activity of methanolic extract of Acalypha indica Linn. Pak. J. Pharm. Sci. 2010 Jul 1; 23(3):256-8.
23.    Govindarajan M, Jebanesan A, Reetha D, Amsath R, Pushpanathan T, Samidurai K. Antibacterial activity of Acalypha indica L. Eur Rev Med Pharmacol Sci. 2008 Sep 1; 12(5):299-302.
24.    Govindarajan M, Jebanesan A, Pushpanathan T, Samidurai K. Studies on effect of Acalypha indica L.(Euphorbiaceae) leaf extracts on the malarial vector, Anopheles stephensi Liston (Diptera: Culicidae). Parasitology Research. 2008 Aug; 103(3):691-5.
25.    Sudhakar C, Vankudothu N, Panjala S, Rao NB, Anupalli RR. Phytochemical analysis, anti-oxidant and anti-microbial activity of Acalypha indica leaf extracts in different organic solvents. International Journal of Phytomedicine. 2016 Nov 9; 8(3):444-52.
26.    Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: a food plant with multiple medicinal uses. Phyther Res. 2007; 21: 17–25.
27.    Yamani HA, Pang EC, Mantri N, Deighton MA. Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria. Frontiers in Microbiology. 2016 May 17; 7:681.
28.    Naik LS, Shyam P, Marx KP, Baskari S, Devi VR. Antimicrobial activity and phytochemical analysis of Ocimum tenuiflorum leaf extract. Int. J. PharmTech Res. 2015; 8(1):88-95.
29.    Islam AK, Kato-Noguchi H. Phytotoxic activity of Ocimum tenuiflorum extracts on germination and seedling growth of different plant species. The Scientific World Journal. 2014 Jul; 2014.doi:10.1155/2014/676242.
30.    Singh D, Chaudhuri PK. A review on phytochemical and pharmacological properties of Holy basil (Ocimum sanctum L.). Industrial Crops and Products. 2018 Aug 1; 118:367-82.
31.    Sahu J, Rathi B, Koul S, Khosa RL. Solanum trilobatum (Solanaceae)-an overview. Journal of Natural Remedies. 2013; 13(2):76-80.
32.    Emmanuel, S., Ignacimuthu, S., Perumalsamy, R. and Amalraj, T. Antiinflammatory activity of Solanum trilobatum. Fitoterapia 77, 611–612 (2006).
33.    Latha PS, Kannabiran K. Antimicrobial activity and phytochemicals of Solanum trilobatum Linn. African Journal of Biotechnology. 2006; 5(23).
34.    Shahjahan M, Sabitha KE, Jainu M, Devi CS. Effect of Solanum trilobatum against carbon tetra chloride induced hepatic damage in albino rats. Indian Journal of Medical Research. 2004 Sep 1; 120:194-8.
35.    Manivannan E, Kothai R, Arul B. Hepatoprotective activity of Solanum trilobatum Linn against CCL4 induced hepatotoxicity in rats. Int. J. Advances in Pharmacy, Biology and Chemistry. 2012; 1(1).
36.    Raja L, Pooshan GV, Venkatachalam VV, Vijayakumar M. Immunomodulatory activity of leaves of Solanum trilobatum in experimental rats. Pharmacologyonline. 2009; 3:758-65.
37.    Govindan S, Viswanathan S, Vijayasekaran V, Alagappan R. A pilot study on the clinical efficacy of Solanum xanthocarpum and Solanum trilobatum in bronchial asthma. Journal of Ethnopharmacology. 1999 Aug 1; 66(2):205-10.
38.    Govindan S, Viswanathan S, Vijayasekaran V, Alagappan R. Further studies on the clinical efficacy of Solanum xanthocarpum and Solanum trilobatum in bronchial asthma. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2004 Oct; 18(10):805-9.
39.    Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology. 2005 Aug 19; 4(7):685-8.
40.    Doss A. Preliminary phytochemical screening of some Indian medicinal plants. Ancient Science of Life. 2009 Oct; 29(2):12.
41.    Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G. Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitology Research. 2009 Apr; 104(5):1163-71.
42.    Paul S, Saha D. Comparative Study of the Efficacy of Barleria prionitis Leaf extracts against Bacteria. Asian J. Pharm. Res. 2012 Jul; 2(3):107-10.
43.    Rajiniraja M, Jayaraman G. Bioautography guided screening of selected Indian medicinal plants reveals potent antimycobacterial activity of Allium sativum extracts-implication of non-sulfur compounds in inhibition. Int J Pharm Pharm Sci. 2014; 6:671-6.
44.    Singh AK, Reyrat JM. Laboratory maintenance of Mycobacterium smegmatis. Current Protocols in microbiology. 2009 Aug; 14(1):10C-.
45.    Vlachos V, Critchley AT, Von Holy A. Establishment of a protocol for testing antimicrobial activity in southern African Macroalgae. Microbios. 1996 Jan 1; 88(355):115-23.
46.    Hammuel C, Anyim BP, Raplong HH, Inekwe VU, Batari ML. Phytochemical screening and in vitro antibacterial activity of methanol and aqueous extracts of Acalypha racemosa leaves. Aceh International Journal of Science and Technology. 2014 Jul 15; 3(2):117-23.
47.    Arun P, Purushotham KG, Jayarani J, Kumari V, Chamundeeswari D. Screening Antibacterial Activity of Various Extracts of Lawsonia inermis. Research Journal of Pharmacognosy and Phytochemistry. 2010 Jun 22; 2(3):185-7.
48.    Dos Santos AC, Marinho VH, Silva PH, Macchi BD, Arruda MS, Silva EO, do Nascimento JL, Sena CB. Microenvironment of Mycobacterium smegmatis Culture to Induce Cholesterol Consumption Does Cell Wall Remodeling and Enables the Formation of Granuloma-Like Structures. BioMed Research International. 2019 Apr 15; 2019.
49.    Krishnaveni M, Kalaivani M, Banu CR, Kumari GK. GC-MS/MS study of Parthenium hysterophorus L (N. Am) stem, antimicrobial activity. Research Journal of Pharmacy and Technology. 2015 May 1; 8(5):517.
50.    Kumar T, Ray S, Brahmachary RL, Ghose M. Preliminary GC-MS analysis of compounds present in the root exudates of three mangrove species. Acta Chromatographica. 2009 Mar 1; 21(1):117-25.
51.    Manikandan P, Ramalingam SM, Vinothini G, Ramamurthi VP, Singh IP, Anandan R, Gopalakrishnan M, Nagini S. Investigation of the chemopreventive potential of neem leaf subfractions in the hamster buccal pouch model and phytochemical characterization. European Journal of Medicinal Chemistry. 2012 Oct 1; 56:271-81.
52.    Doss A, Dhanabalan R. Preliminary Phytochemical Screening and Antibacterial Studies of Leaf Extract of Solanum trilobatum Linn. Ethnobotanical Leaflets. 2008; 2008(1):85.
53.    Nagarajan SM, Kandasamy S, Chinnappa R. Comparative antimicrobial activity of callus and natural plant extracts of Solanum trilobatum L. Ancient Science of Life. 2009 Jan; 28(3):3.
54.    Bange FC, Collins FM, Jacobs WR. Survival of mice infected with Mycobacterium smegmatis containing large DNA fragments from Mycobacterium tuberculosis. Tubercle and Lung Disease. 1999 Jun 1; 79(3):171-80.
55.    Goins CM, Schreidah CM, Dajnowicz S, Ronning DR. Structural basis for lipid binding and mechanism of the Mycobacterium tuberculosis Rv3802 phospholipase. Journal of Biological Chemistry. 2018 Jan 26; 293(4):1363-72.
56.    Chandran AV, Jayanthi S, Vijayan M. Structure and interactions of RecA: plasticity revealed by molecular dynamics simulations. Journal of Biomolecular Structure and Dynamics. 2018 Jan 2; 36(1):98-111.
57.    Yamada H, Yamaguchi M, Igarashi Y, Chikamatsu K, Aono A, Murase Y, Morishige Y, Takaki A, Chibana H, Mitarai S. Mycolicibacterium smegmatis, basonym Mycobacterium smegmatis, expresses morphological phenotypes much more similar to Escherichia coli than Mycobacterium tuberculosis in quantitative structome analysis and CryoTEM examination. Frontiers in Microbiology. 2018 Sep 11; 9:1992.
58.    Gahleitner F, Guallar-Hoyas C, Beardsmore CS, Pandya HC, Thomas CP. Metabolomics pilot study to identify volatile organic compound markers of childhood asthma in exhaled breath. Bioanalysis. 2013 Sep; 5(18):2239-47.
59.    Rajkumar V, Gunasekaran C, Dharmaraj J, Chinnaraj P, Paul CA, Christy IK. Insecticidal property of Solanum trilobatum L. Extract against stored-grain pests. Int. J. Adv. Res. Biol. Sci. 2018; 5(9):145-55.
60.    Babu A, Anand D, Saravanan P. Phytochemical analysis Ficus arnottiana (Miq.) Miq. leaf extract using GC–MS analysis. Int J Res Phytochem Phar‑macol Res. 2017; 9(6):775-9.
61.    Badwaik H, Sonkar S, Singh M, Rajpal S, Sisodiya D, Pandey A. Synthesis and Antibacterial Evaluation of Some Novel Phenylthiazolyl-Quinqzolin-4 (3h)-One Derivative. Asian Journal of Research in Chemistry. 2009 Dec 28; 2(4):544-6.
62.    Rane YS, Varma RR, Patil LS, Athlekar SV, Chowdhary AS, Bobade AS. Synthesis and antimicrobial activity of 5-substituted-2-(1-H-benzimidazole) sulfonamides. Asian Journal of Research in Chemistry. 2010 Jun 28; 3(2):335-8.

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