Author(s): Inas Sattar Abd, Ahmed S. Mohammed, Mohammed Flyyiah Treaf

Email(s): inasabd4@gmail.com

DOI: 10.52711/0974-360X.2022.00909   

Address: Inas Sattar Abd1*, Ahmed S. Mohammed2, Mohammed Flyyiah Treaf2
1Department of Microbiology, College of Science, Al-Karkh University of Science, Baghdad, Iraq.
2College of Health and Medical Technology, Middle Technical University, Baghdad, Iraq.
*Corresponding Author

Published In:   Volume - 15,      Issue - 12,     Year - 2022


ABSTRACT:
S. typhi is causes typhoid in Iraq and developing countries. The abuse of antibiotics make the patient infected with S. typhi wich is resistant to many antibiotics. Aims of the study: Molecular and phylogenetic tree of genomic resistance genes associated Aminoglycoside nucleotidyltransferase (aad-A1), and detection mutation occur in aadA1 gene. Disc diffusion method and VITEK2 compact system to detect the resistance of S.typhi to 11 antibiotics and using the PCR technique to detect the prevalence of aadA1 resistance gene. The genomic DNA extracted from all S. typhi isolated harboring resistance gene. The prevalence of the aadA1 gene was 12(24%) from all S. typhi isolates and the presence of a silent mutation in sample number 2 Iraqi isolate. The first isolate (MW805237.1) was almost just like the isolates of each of the countries Korea, Iran Iran, India, and Madagascar, but The second Iraqi isolate (MW805238.1) was the share of congruence between them at 99% at position G. The aadA1 gene was present in S. typhi isolated from blood, the stool of human, and the detected mutation has no effect.


Cite this article:
Inas Sattar Abd, Ahmed S. Mohammed, Mohammed Flyyiah Treaf. Whole-genome sequencing of Aminoglycoside nucleotidyltransferase aadA1 in patients with typhoid. Research Journal of Pharmacy and Technology2022; 15(12):5393-6. doi: 10.52711/0974-360X.2022.00909

Cite(Electronic):
Inas Sattar Abd, Ahmed S. Mohammed, Mohammed Flyyiah Treaf. Whole-genome sequencing of Aminoglycoside nucleotidyltransferase aadA1 in patients with typhoid. Research Journal of Pharmacy and Technology2022; 15(12):5393-6. doi: 10.52711/0974-360X.2022.00909   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-12-3


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