Inas Sattar Abd, Ahmed S. Mohammed, Mohammed Flyyiah Treaf
Inas Sattar Abd1*, Ahmed S. Mohammed2, Mohammed Flyyiah Treaf2
1Department of Microbiology, College of Science, Al-Karkh University of Science, Baghdad, Iraq.
2College of Health and Medical Technology, Middle Technical University, Baghdad, Iraq.
Volume - 15,
Issue - 12,
Year - 2022
S. typhi is causes typhoid in Iraq and developing countries. The abuse of antibiotics make the patient infected with S. typhi wich is resistant to many antibiotics. Aims of the study: Molecular and phylogenetic tree of genomic resistance genes associated Aminoglycoside nucleotidyltransferase (aad-A1), and detection mutation occur in aadA1 gene. Disc diffusion method and VITEK2 compact system to detect the resistance of S.typhi to 11 antibiotics and using the PCR technique to detect the prevalence of aadA1 resistance gene. The genomic DNA extracted from all S. typhi isolated harboring resistance gene. The prevalence of the aadA1 gene was 12(24%) from all S. typhi isolates and the presence of a silent mutation in sample number 2 Iraqi isolate. The first isolate (MW805237.1) was almost just like the isolates of each of the countries Korea, Iran Iran, India, and Madagascar, but The second Iraqi isolate (MW805238.1) was the share of congruence between them at 99% at position G. The aadA1 gene was present in S. typhi isolated from blood, the stool of human, and the detected mutation has no effect.
Cite this article:
Inas Sattar Abd, Ahmed S. Mohammed, Mohammed Flyyiah Treaf. Whole-genome sequencing of Aminoglycoside nucleotidyltransferase aadA1 in patients with typhoid. Research Journal of Pharmacy and Technology2022; 15(12):5393-6. doi: 10.52711/0974-360X.2022.00909
Inas Sattar Abd, Ahmed S. Mohammed, Mohammed Flyyiah Treaf. Whole-genome sequencing of Aminoglycoside nucleotidyltransferase aadA1 in patients with typhoid. Research Journal of Pharmacy and Technology2022; 15(12):5393-6. doi: 10.52711/0974-360X.2022.00909 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-12-3
1. Elumalai,S., G. Muthu, R.E.M. Selva and S.Ramesh, Detection of TEM, SHV and CTX-M-type B-lactamase production among clinical isolates of Salmonella species. J.Med. Microbial., 2014, 63: 962-967.
2. Barlow M, Hall BG. Origin and evolution of the AmpC beta-lactamases of Citrobacter freundii. Antimicrob Agents Chemother. 2002;46(5):1190–1198. doi:10.1128/AAC.46.5.1190-1198.2002
3. Majowicz SE, Musto J, Scallan E, et al. The global burden of nontyphoidal Salmonella gastroenteritis. Clin Infect Dis. 2010; 50(6): 882–889. doi:10.1086/650733
4. Crump JA, Lubsy SP, Mintz ED. The global burden of enteric fever. Bull World Health Organ 2004; 82: 346–53
5. Parry CM, Hien TT, Dougan G, White NJ, Farrar JJ. Typhoid fever. N Engl J Med 2002;347:1770-82. PUBMED | CROSSREF
6. Lee K.H., Yoo J.R., Kim Y.R, and Heo S.T. Phylogenetic Analysis for the Origin of Typhoid Fever Outbreak on Jeju Island, Korea, in 2017. Infect Chemother. 2020; 52(3): 421-426.
7. Soheila Moradi Bidhendi, Ali Mojtahedi, Farshideh Alaei. Identification and Serotyping of Salmonella Strains from poultry by PCR-RFLP in Shiraz, Iran. Research J. Pharm. and Tech 2018; 11(6): 2591-2594.
8. Kishnani Khushboo, Bhandari Saloni, Rathore Kamal Singh. A Briefing of a Global Crisis: Antibiotic Resistance. Asian J. Res. Pharm. Sci. 2020; 10(4): 264-272.
9. Gurudharshini Natarajan, Madhumitha Muthusamy, Muthusaravanan Sivaramakrishnan, Perianayaki Periasamy, Poornimmashree A, Kumaravel Kandaswamy. A Big Picture on Antimicrobial Strategies then and now. Research J. Engineering and Tech. 2017; 8(4): 361-364.
10. Suresh A. Marnoor. A Review on Antimicrobial Resistance and Role of Pharmacist in tackling this Global Threat. Res. J. Pharm. Dosage Form. & Tech. 2017; 9(4): 143-146.
11. Wal Pranay, Wal Ankita, Srivastava Rishabh, Rastogi Prateek, Rai Awani K. Antibiotic Therapy in Pediatric Patients. Research J. Pharm. and Tech. 3(1): Jan.-Mar. 2010; Page 118-120.
12. Saurabh Bharadwaj, U.V.S. Teotia, Kishan Singh, Rajib Sharma, Yogendra Singh. Effect of Antibiotic on Various Microorganisms Isolated from Nosocomial Infected Patients in General Hospital. Research J. Pharm. and Tech. 7(4): April, 2014; Page 408-414.
13. G M. Vaishali, R.V. Geetha. The Superbug Threat. Research J. Pharm. and Tech. 8(3): Mar., 2015; Page 343-346.
14. Mingeot-Leclercq MP, Glupczynski Y, Tulkens PM. Aminogly¬cosides: activity and resistance. Antimicrob Agents Chemother. 1999;43(4):727–37.
15. Kotra LP, Haddad J, Mobashery S. Aminoglycosides: perspectives on mechanisms of action and resistance and strategies to coun¬ter resistance. Antimicrob Agents Chemother. 2000;44(12):3249–56.
16. Fluit AC, Visser MR, Schmitz FJ. Molecular detection of antimicro¬bial resistance. Clin Microbiol Rev. 2001;14(4):836–71.
17. Akhilesh Gupta. Plazomicin: A step toward next generation aminoglycosides. Review. Asian J. Res. Pharm. Sci. 2017; 7(3):173-180.
18. Akhilesh Gupta. Plazomicin: A step toward next generation aminoglycosides. Review. Asian J. Res. Pharm. Sci. 2017; 7(3):173-180.
19. Frye JG and Jackson CR. Genetic mechanisms of antimicrobial resistance identified in Salmonella enterica, Escherichia coli, and Enteroccocus spp. isolated from U.S. food animals”. Frontiers in Microbiology, 2013; 4: 1-22.
20. Vanka Kanth Swaroop, Aparna Mukherjee, Sumit Sharma, William Jabez Osborne. Isolation and characterization of drug resistant Salmonella typhi from sewage water. Research J. Pharm. and Tech. 8(2): Feb. 2015; Page 167-171.
21. Bancer., Doudai., Thong K.L., Watanabi H., and Puthucheary S.D. Characterization of drug resistant Salmonella enterica serovar typhimurium by antibiograms, plasmids, integrons, resistance gene, and PFGE. Journal of Microbiology and biotechnology, 2010; 20(6):1042-52.
22. Doosti A., Jami. M.S., and Farsani A.M. Prevalence of aadA1, aadA2,aadAB, strA and strB genes and their associations with multidrug resistance phenotype in Salmonella.Thai J Vet Med. 2016; 46(4):691-697.
23. Ma M., Wang H, Yu Y., Zhang D and Liu S., Detection of antimicrobial resistance genes of pathogenic Salmonella from swine with DNA microarray. J Vet Digan Invest. 2007; 19(2):161-167.
24. Afzal A., Sarwa Y., Ali A., Moqbool A., Salman M., Habeeb M.A and Haque A. Molecular evaluation of drug resistance in clinical isolates of Salmonella enterica serovar Typhi from Pakistan. J Infect Dev Ctries; 2013; 7(12):929-940.
25. Alcaine S.D., Warnick L.D., and Wiedmann M., Antimicrobial Resistance in Nontyphoidal Salmonella, Review. Journal of Food Protection, 2007; Vol. 70, No. 3, Pages 780–790 Copyright, International Association for Food Protection.
26. Mascaretti, O. A. Bacteria versus antimicrobial agents: an integrated approach. ASM Press, Washington, D.C. 2003.
27. Chen, S., S. Zhao, D. G. White, C. M. Schroeder, R. Lu, H. Yang, P. F. McDermott, S. Ayers, and J. Meng. Characterization of multiple-antimicrobial-resistant Salmonella serovars isolated from retail meats. Appl. Environ. Microbiol. 2004; 70:1–7
28. Pezzella, C., A. Ricci, E. DiGiannatale, I. Luzzi, and A. Carattoli. Tetracycline and streptomycin resistance genes, transposons, and plasmids in Salmonella enterica isolate from animals in Italy. Antimicrob. Agents Chemother. 2004; 48:903–908.
29. Frech, G., C. Kehrenberg, and S. Schwarz. Resistance phenotypes and genotypes of multiresistant Salmonella enterica subsp. enterica serovar Typhimurium var. Copenhagen isolates from animal sources. J. Antimicrob. Chemother. 2003; 51:180–182
30. Potenski, C. J., M. Gandhi, and K. R. Matthews. Exposure of Salmonella Enteritidis to chlorine or food preservatives decreases susceptibility to antibiotics. FEMS Microbiol. Lett. 2003; 220:181–186.
31. Winokur, P. L., D. L. Vonstein, L. J. Hoffman, E. K. Uhlenhopp, and G. V. Doern. Evidence for transfer of CMY-2 AmpC beta-lactamase plasmids between Escherichia coli and Salmonella isolates from food animals and humans. Antimicrob. Agents Chemother. 2001; 45:2716–2722.
32. Becker, B., and Cooper, M. A. Aminoglycoside antibiotics in the 21st century. ACS Chem. Biol. 2013; 8, 105–115 CrossRef Medline