Author(s): Anuradha N. Chivate, Prathibha S. Salve, Rajendra C. Doijad, Avinash M. Mane, Niranjan D. Chivate

Email(s): anc_20@rediffmail.com

DOI: 10.52711/0974-360X.2022.00923   

Address: Anuradha N. Chivate1*, Prathibha S. Salve2, Rajendra C. Doijad3, Avinash M. Mane4, Niranjan D. Chivate5
1,2,4Department of Pharmacology, Krishna Institute of Medical Sciences, Malkapur, Karad Maharashtra - 415539 India.
3Department of Pharmaceutics, Krishna Institute of Pharmacy, Malkapur, Karad. Maharashtra - 415539 India.
5Department of Pharmaceutics, KCT’S Krishna College of Pharmacy, Malkapur, Karad, Maharashtra - 415539 India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 12,     Year - 2022


ABSTRACT:
Introduction:- Capecitabine resealed erythrocytes were prepared as a potential targeted drug delivery system for the treatment of hepatocellular carcinoma. This system has been developed to increase the therapeutic efficacy of drug and to reduce its adverse effects. This paper demonstrated the acute toxicity study of the formulation in Swiss albino mice. Methodology:- Dose of Capecitabine was 2000 mg/kg orally with zero toxicity as per the previous studies. The doses of formulation selected were 1/5th, 1/10th, 1/20th of the oral dose calculated on the basis of the % encapsulation efficiency of drug in formulation. The doses were given intravenously in single dose in mice tail vein. All the animals were observed for 14 days period for mortality, behavioral symptoms and locomotor activity. Results:- After sacrification of animals, serum was collected to analyze hematological and biochemical parameters. The isolated liver was observed for histopathological changes in mice. Here, we found that all the three to be selected would be safe for further in- vivo screening of Hepatocellular carcinoma. Conclusion:- So, we concluded that, all the three doses of formulation low (0.90mg/kg), medium (1.79mg/kg) and high (3.58mg/kg) with no signs of mortality, behavioural changes and no necrosis in liver histopathology. This revealed that, all the three selected doses were safe for further in vivo studies.


Cite this article:
Anuradha N. Chivate, Prathibha S. Salve, Rajendra C. Doijad, Avinash M. Mane, Niranjan D. Chivate. Acute Toxicity Study of Intravenously Administered Capecitabine Resealed Erythrocytes in Mice. Research Journal of Pharmacy and Technology2022; 15(12):5473-7. doi: 10.52711/0974-360X.2022.00923

Cite(Electronic):
Anuradha N. Chivate, Prathibha S. Salve, Rajendra C. Doijad, Avinash M. Mane, Niranjan D. Chivate. Acute Toxicity Study of Intravenously Administered Capecitabine Resealed Erythrocytes in Mice. Research Journal of Pharmacy and Technology2022; 15(12):5473-7. doi: 10.52711/0974-360X.2022.00923   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-12-17


REFERENCES:
1.    Parashuraman S. Toxicological screening. Educatuional forum.  Journal of Pharmacology and  Pharmacotherapeutics. 2011 Jul 27; 2(2): 74-79. doi: 10.4103/0976-500X.81895
2.    Selvan T, Suresh R., Subramanian NS, Ramadevi M. Anxiolytic Effect of Polyherbal Extracts on Rodents Res.  J. Pharmacognosy and Phytochem. 7(1): Jan.-Mar. 2015; 01-05. doi: 10.5958/0975-4385.2015.00001.1.
3.    Jijith US, C. R. Sudhakaran Nair, K. Pramod. Acute toxicity and Gross Behavioural effects of Alcoholic Extract of Anamirta cocculus willd.Research J. Pharmacology and Pharmacodynamics. 2016; 8(1): 1-4. doi: 10.5958/2321-5836.2016.00001.X.
4.    Tanuja Yadav, Sachin Rohane. Acute Toxicity study of Synthesized drug and Herbal Product. Asian Journal of Pharmaceutical Research. 2021; 11(4):251-6. doi: 10.52711/2231-5691.2021.00044
5.    Akhila JS, Shyamjith, Deepa and Alwar M. C. Acute toxicity studies and determination of median lethal dose. Current Science. 2007 Jul 17; 93(7): 917-920. doi: https://www.jstor.org/stable/24099255.
6.    Parimalakrishnan S. Sundar VD, Ulaganathan I, Nandhakumar S, Dey A. Preliminary Toxicity Evaluation of Bidens pilosa Extracts Using Experimental Mice. Research J. Pharmacognosy and Phytochemistry 2010; 2(5): 397-402.
7.    Vijayan PS, Rekha PD, Dinesh U, Arun AB. Determination of acute lethal dose 50 (LD50) of uranyl nitrate in male swiss albino mice. Research J. Pharm. and Tech. 2018; 11(3): 1086-1088. doi: 10.5958/0974-360X.2018.00203.2
8.    Shejwal KP, Randive DS, Bhinge SD, Bhutkar MA, Wadkar GH, Jadhav NR. Green synthesis of silver and nanoparticles of isolated proanthocyanidin, its characterization, antioxidant, antimicrobial and cytotoxic activities against COLO320DM and HT29. J. Genet. Eng. Biotechnol. 2020; 18(43): 1-11. http://doi.org/10.1186/s43141-020-00058-2.Todkar SS, Bhutkar MA, Randive DS, Wadkar GH, Bhinge SD, Risk and management of Nanoparticles- an overview. Int. J. Phar. Res. Life Sci. 2017; 1:1-13.
9.    Yue X et al. Acute toxicity of nickel nanoparticles in rats after intravenous injection International Journal of Nanomedicine. 2014 Mar 12; 9: 1393–1402. doi: https://doi.org/10.2147/IJN.S56212.
10.    Li SX et al. Acute and Sub-chronic Toxicity of Tetrandrine in Intravenously Exposed Female BALB/c Mice. Chinese Journal of Integrative Medicine. 2015 Oct 29; 1-7. doi: 10.1007/s11655-015-2303-2.
11.    S. Vijayalakshmi, K. G. Kripa, Suganthi V. Toxicological Study of Hydroalcoholic Extract of Blepharis maderaspatensis (L.) Heyne ex Roth in Wistar Albino Rats. Research J. Pharm. and Tech. 2020; 13(12):5673-5679. doi: 10.5958/0974-360X.2020.00988.9
12.    Lingyun X, Acute and subacute toxicity studies on triptolide and triptolide-loaded polymeric micelles following intravenous administration in rodents. Food and Chemical Toxicology. 2013 Apr 11; 57: 371–379. doi: 10.1016/j.fct.2013.03.044.
13.    Jiaying X. Acute Toxicity of Intravenously Administered Titanium Dioxide Nanoparticles in Mice. PLOS ONE. 2013 Aug 8; 8(8): 1-6. https://doi.org/10.1371/journal.pone.0070618.
14.    Sullivan DW, Peterson RC, CV Mujer, SC Gad. A 7-Day Intravenous Toxicity Study and Neurotoxicity Assessment of Pyridorin in Sprague-Dawley Rats. Human and Experimental Toxicology. 2016 Aug 9; 1–9. doi: 10.1177/0960327116661023.
15.    Sengottuvelu S, Srinivasan D, Ramasamy S. Toxicological Evaluation of a Novel Anti-Ulcer Herbal Formulation. Research J. Pharm. and Tech. 1(3): July-Sept. 2008; Page 207-210. doi: 10.5958/0974-360X.
16.    Pramod V. Burakale, Manish R. Bhise, Dinesh M. Sakarkar, Suresh G. Sudke. Synthesis and Assessment of Sub-acute Toxicity of Novel Rosin Esters of Polyethylene Glycol 200 in Swiss Albino Mice. Research Journal of Pharmacy and Technology. 2021; 14(4):1859-6. doi: 10.52711/0974-360X.2021.00329
17.    Gajendra Pratap Choudhary, Ashutosh Pal Jain. Evaluation of Acute, Subacute and LD50 values of Methanolic extract of Sphaeranthus indicus leaves in Albino mice. Research Journal of Pharmacy and Technology. 2021; 14(5):2487-2. doi: 10.52711/0974-360X.2021.00438
18.    Meena Rajarethinam, Ramaswamy Selvaratnam Ramaswamy. Long term toxicity study of a Siddha formulation Kandhaga rasayanam (KR) in Rats. Research J. Pharm. and Tech 2018; 11(4): 1640-1646. doi: 10.5958/0974-360X.2018.00305.0
19.    Yu Y et al. Acute Toxicity of Amorphous Silica Nanoparticles in Intravenously Exposed ICR Mice. PLOS ONE. 2013 Apr 12; 8: e61346 1-16. doi:10.1371/journal.pone.0061346.
20.    Alipour M, Milton GS, Pucaj K and Suntres ZE. Acute Toxicity Study of Liposomal Antioxidant Formulations Containing N-Acetylcysteine, Α-Tocopherol, And Γ-Tocopherol In Rats. Journal of Liposome Research 2012 Mar 9; 22(2): 158–167. doi: 10.3109/08982104.2012.662654.
21.    Abdallaa A, Nyamathullab S, Shamsuddinb N, Norhafiza MA, Kein SM, Khalijah A, Noor HN Osman Y et al. Acute And 28-Day Sub-Acute Intravenous Toxicity Studies of 1-S-1-Acetoxychavicol Acetate in Rats. Toxicology and Applied Pharmacology. 2018 Aug 21; 356 204–213. doi: 10.1016/j.taap.2018.08.014.
22.    Ouidad A, Sara C, Samir D. Biological properties and Acute Toxicity Study of Copper oxide nanoparticles prepared by aqueous leaves extract of Portulaca oleracea (L). Asian J. Pharm. Res. 2020 Mar 30; 10(2):89-94. doi: 10.5958/2231-5691.2020.00017.9
23.    Donkor K, Okine LNK, Abotsi WKM, Woode E.  Acute and Sub-Chronic Toxicity Studies of Aqueous Extract of Root Bark of Cassia Sieberiana D.C. in Rodents. Journal of Applied Pharmaceutical Science. 2014 Apr 22; 4 (04): 084-089. doi:10.7324/JAPS.2014.40415.
24.    Ronald Darwin, C. Vijaya, K. Sujith, M. Sadhavi, Haneesha Pothuri. Acute and sub-acute toxicological evaluation of aqueous and ethanol fractions of Annona squamosa root a traditional medicinal herb. Research J. Pharm. and Tech. 4(9): Sept. 2011; Page 1475-1479.
25.    Jain VK, Ahirwar D, Jain B, Ahirwar B. Evaluation of Acute Oral Toxicity and Mast Cell Degranulation of an aqueous ethanolic extract of Tritium aestivum Linn. Research J. Pharm. and Tech 2018 Dec 24; 11(2):643-648. doi: 10.5958/0974-360X.2018.00120.8.
26.    Shunmugaram S. Physiochemical and Acute Toxicity Analysis of a Siddha Herbo-Mineral Formulation – Pachai Karpoora Vadagam (PKV). Research J. Pharm. and Tech 2019 Nov 21; 12(1): 227-230. doi: 10.5958/0974-360X.2019.00042.8.
27.    Abdallaa A et al. Acute And 28-Day Sub-Acute Intravenous Toxicity Studies of 1-S-1-Acetoxychavicol Acetate in Rats. Toxicology and Applied Pharmacology. 2018 Aug 21; 356 204–213. doi: 10.1016/j.taap.2018.08.014.
28.    Donkor K, Okine LNK, Abotsi WKM, Woode E.  Acute and Sub-Chronic Toxicity Studies of Aqueous Extract of Root Bark of Cassia Sieberiana D.C. in Rodents. J App. Pharm. Sci. 2014 April 22; 4 (04): 084-089. doi:10.7324/JAPS.2014.40415.
29.    Shakib AM, Hashemzadeh MS, Ali A, Oraei M, Mirshafiey A. Evaluation Of The Acute And 28-Day Sub-Acute Intravenous Toxicity of Α-L-Guluronic Acid (ALG) in Mice. Drug and Chemical Toxicology. 2019 Sept 19: 1-11. https://doi.org/10.1080/01480545.2019.1665679.
30.    Darwin CR, Vijaya C, Sujith K, Sadhavi M, Pothuri H. Acute and sub-acute toxicological evaluation of aqueous and ethanol fractions of Annona squamosa root a traditional medicinal herb. Research J. Pharm. and Tech. Jul 7 2011; 4(9): 1475-1479. doi:10.1142/S0192415X17500501.

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