Manas Ranjan Sahoo, Marakanam Srinivasan Umashankar, Ramesh Raghava Varier
Manas Ranjan Sahoo1, Marakanam Srinivasan Umashankar1*, Ramesh Raghava Varier2
1Department of Pharmaceutics, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamilnadu, India, 603203.
2AVN Ayurveda Formulation Pvt Ltd, Madurai, Tamilnadu, India, 600034.
Volume - 15,
Issue - 11,
Year - 2022
The throat lozenegs are most commonly used first line of treatment for relief of various throat infections. The most common ingredient used in the lozenegs are various herbal extracts and essential oil having local action of throat area. in the present studies a essential oil based lozenges were prepared using eucalyptus oil and menthol as active ingredient. Antibacterial activity was evaluated for menthol and eucalyptus oil against human pathogens E.coli and S.aureus. both the sugar based and sugar free base was used to develop two types of lozenges formulation. Quality parameters were evaluated for lozenges formulations such as hardness, friability, dissolving time, and moisture content. Compatibility study was carried out using thin layer chromatography and gas chromatography analysis. The eucalyptus oil and menthol displayed moderate antibacterial activity against E.coli and S.aureus. The lozenges were found to be having satisfactory quality parameters. GC and TLC analysis was found to be a suitable method for identification of menthol and eucalyptus oil in the lozenges. TLC was found to be a simple, quick and simple technique for rapid identification of the essential oil and to confirm the batch-to-batch consistency. 1H-NMR and 13C-NMR was found to be useful tool for rapid identification of essential oils on basis of diagonostic peaks. Overall this study can be used for standard methods for quality control of the essential lozeneges formulation for the new formulation and product available in the market as well.
Cite this article:
Manas Ranjan Sahoo, Marakanam Srinivasan Umashankar, Ramesh Raghava Varier. Development and Evaluation of Essential Oil-based Lozenges using Menthol and Eucalyptus and in vitro Evaluation of their Antimicrobial activity in S.aureus and E.coli. Research Journal of Pharmacy and Technology. 2022; 15(11):5283-8. doi: 10.52711/0974-360X.2022.00890
Manas Ranjan Sahoo, Marakanam Srinivasan Umashankar, Ramesh Raghava Varier. Development and Evaluation of Essential Oil-based Lozenges using Menthol and Eucalyptus and in vitro Evaluation of their Antimicrobial activity in S.aureus and E.coli. Research Journal of Pharmacy and Technology. 2022; 15(11):5283-8. doi: 10.52711/0974-360X.2022.00890 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-11-75
1. Morokutti-Kurz M. Graf C. Prieschl-Grassauer E. Amylmetacresol/2,4-dichlorobenzyl alcohol, hexylresorcinol, or carrageenan lozenges as active treatments for sore throat. International Journal of General Medicine 2017; 10: 53–60. doi: 10.2147/IJGM.S120665
2. Sousa R. Lakha DR. Brette S. Hitier S. A Randomized, Double-Blind, Placebo-Controlled Study to Assess the Efficacy and Safety of Ambroxol Hard-Boiled Lozenges in Patients with Acute Pharyngitis. Pulm Ther, 2019; 5:201–211. doi: 10.1007/s41030-019-00100-w
3. Sahoo MR. Umashankar MS, Varier RR. The research updates and prospects of herbal hard-boiled lozenges: a classical platform with promising drug delivery potential. International journal of applied pharmaceutics 2021; 13(2): 1-13. doi.org/10.22159/ijap.2021v13i2.40165
4. Yousuf Ali M. Shamim Qureshi M. Hamed M. Das B, Rao KP. Design and Evaluation of Nimesulide Lozenges for Pediatrics. Research Journal of Pharmacy and Technology 2010; 3 (3):818-20. doi:10.5958/0974-360X
5. Bhandarkar A, Alexander A, Bhatt A, Sahu P. Agrawal P. Tripti B et al. Evaluation of Ascorbic acid Lozenges for the treatment of Oral Ulcer. Research Journal of Pharmacy and Technology 2018; 11(4):1307-12. doi:10.5958/0974-360X.2018.00243.3
6. Pravalika L. Shravan Kumar Y. Formulation and Evaluation of Theophylline Lozenges. Research Journal of Pharmacy and Technology 2021; 14(3):1601-6. doi:10.5958/0974-360X.2021.00284.5
7. Wise PM, Paul AS. Dalton P. Sweet Taste and Menthol Increase Cough Reflex Thresholds. Pulm Pharmacol Ther 2012; 25(3): 236-41. doi:10.1016/j.pupt.2012.03.005
8. Addey D. Shephard A. Incidence, causes, severity and treatment of throat discomfort: a four-region online questionnaire survey. BMC Ear Nose Throat Disorder 2012; 12: doi:10.1186/1472-6815-12-9
9. Valussi M. Antonelli M. Donelli D. Firenzuoli F. Appropriate use of essential oils and their components in the management of upper respiratory tract symptoms in patients with COVID-19. J Herb Med. 2021; 28: 100451. doi: 10.1016/j.hermed.2021.100451.
10. Da Silva JK. Figueiredo PL. Byler KG. Setzer WN. Essential Oils as Antiviral Agents, Potential of Essential Oils to Treat SARS-CoV-2 Infection: An In-Silico Investigation. International Journal of Molecular Sciences 2020; 21(10): 3426. doi: 10.3390/ijms21103426.
11. Kamilla A. Bencsik T. Boszormenyi A. Kocsis B. Horvath G. Essential Oils and their vapours as potential antibacterial agents against respiratory tract pathogens. Natural Product Communication 2016; 11(11):1709-12. PMID: 30475513
12. Fischer J. Dethlefsen U. Efficacy of cineole in patients suffering from acute bronchitis: a placebo-controlled double-blind trial Cough. 2013; 9: 25. doi: 10.1186/1745-9974-9-25
13. Sahoo MR. Umashankar MS. Varrier RR. Formulation Development of Curcumin Loaded Lozenges: Extensive Characterization and In Vitro Evaluation of Antioxidant Activity, Anti-Inflammation and Antimicrobial Activity. International Journal of Pharmaceutical Research 2020; 12(2):2706-16. doi: https://doi.org/10.31838/ijpr/2020.SP2.310
14. Ghaffar A. Yameen M. Kiran S. Kamal S. Jalal F. Munir B et al. Chemical Composition and in-Vitro evaluation of the antimicrobial and antioxidant activities of essential oils extracted from seven eucalyptus species. Molecules 2015; 20: 20487-98. doi: 10.3390/molecules201119706
15. Gusain P. Ohki S. Hoshino K. Tsujino Y. Shimokawa N. Takagi M. Chirality-Dependent Interaction of d- and l-Menthol with Biomembrane Models. Membranes 2017; 7(4): 69. doi: 10.3390/membranes7040069.