Gururaja MP, Nidhi P Shetty, Himanshu Joshi
Gururaja MP1*, Nidhi P Shetty1, Himanshu Joshi2
1Department of Pharmacology, NGSM Institute of Pharmaceutical Sciences, NITTE (Deemed to be University), Deralakatte, Mangaluru, Karnataka, India-575018.
2College of Pharmacy, Graphic Era Hill University, Bhimtal campus, Uttarakhnd, India- 263136.
Volume - 15,
Issue - 11,
Year - 2022
The present study was undertaken to evaluate the effect of Panchagavya ghrita on experimentally induced anxiety in swiss albino mice. The study was carried out with an objective of scientifically validating the traditional claims and to compare the ancient knowledge with the latest pharmacological aspects of preclinical research. The Swiss albino mice were divided into 4 groups of 6 mice each. First group received vehicle 10mg/kg orally. Second group was treated with the standard drug diazepam (2mg/kg i.p) one hour before the experiment. Third and fourth group animals were treated with the two different doses of Panchagavya ghrita i.e., 4680mg/kg p.o and 6840mg/kg p.o once daily for 14 days. On the 15th day one hour after administration of the test dose, anti anxiety activity was evaluated using Elevated Plus maze model, Light Dark Transition model & Open field test. Animals treated with Panchagavya Ghrita showed significant result by increasing the number of entries into the open arm and time spent in open arm in Elevated Plus maze model. In Light & Dark Transition model, it showed increase in the Number of entries into the light chamber and time spent in the light chamber and in the open field test, the number of rearings, time spent in the central square and the number of squares crossed increased thus proving the antianxiety activity of Panchagavya ghrita.
Cite this article:
Gururaja MP, Nidhi P Shetty, Himanshu Joshi. Attenuation of Experimentally Induced Anxiety by Panchagavya Ghrita in Mice. Research Journal of Pharmacy and Technology. 2022; 15(11):4897-0. doi: 10.52711/0974-360X.2022.00822
Gururaja MP, Nidhi P Shetty, Himanshu Joshi. Attenuation of Experimentally Induced Anxiety by Panchagavya Ghrita in Mice. Research Journal of Pharmacy and Technology. 2022; 15(11):4897-0. doi: 10.52711/0974-360X.2022.00822 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-11-7
1. Yadav A V, Kawale LA, Nade V S. Effect of Morus alba L. (mulberry) leaves on anxiety in mice. Indian J Pharmacol. 2008;(40): 32.
2. Pavithra H Dave, V. Vishnupriya, R. Gayathri. Herbal Remedies for Anxiety and Depression- A Review. Research J. Pharm. and Tech. 2016; 9(8):1253-1256.
3. Rabbani M, Sajjadi S, Ezarei HR. Anxiolytic effects of Stachys lavandulifolia on the elevated plus- maze model of anxiety in mice. J Ethnopharmacol. 2003; 89: 271-276.
4. Habibur Rahman, M. Chinna Eswaraiah, Anoosha T., Nagaveni K., Manjula K. Evaluation for CNS Activities of Hexane Extract of Citrullus lanatus Seeds. Research J. Pharm. and Tech. 6(8): August 2013; Page 878-884.
5. G.S Achilya ,N.R Kotagale, S.G Wadodkar, A.K Dorle. Hepatoprotactive activity of Panchagavya ghrita against carbon tetrachloride induced Hepatotoxicity in Rats. Indian Journal of Pharmacology. 2003; 35(1):308-311.
6. M.Jithesh. Panchagavya Ghrita A Promising Drug in Ayurvedic Psychiatry.Asian Journal of Pharmaceutical Research and Development. 2013 May-June;1(3):7-15.
7. Sarnga dhar-Samhitha (A treatise on Ayurveda).3rd edition., Chaukhambha Orientalia Publications 1997. )
8. H.Gerhard Vogel (Ed.) Drug Discovery and Evaluation: Pharmacological assays. 2nd ed. Germany. Springer. 2008; 2;(E): 622-626.
9. Divneet Kaur, Richa Shri, Anjoo Kamboj. Evaluation of anti-anxiety effect of Brassica oleracea L. extracts in experimental animals. Pharmacogn J. 2007; 9(5): 638-643.
10. Zeena Fernandes, Prasanna Shama Khandige, Ullas Prakash D’Souza. Anxiolytic potential of Perseaamericana M. by elevated plus maze test. Research J. Pharm. and Tech. 2020; 13(7): 3326-3328.
11. Sanjay Singh Bhandari, Mahaveer Prasad Kabra. To evaluate anti-anxiety activity of thymol. Journal of Acute Disease- 2014;136-140.
12. Ullas Prakash D’Souza, Himanshu Joshi, Nabeesa Alsheena. Anti-anxiety effect of Aerva lanata (L.) using mice model. Research J. Pharm. and Tech. 2020; 13(2):565-568.
13. B.S.Thippeswamy, Brijesh Mishra, V.P Veerapur, Gourav Gupta. Anxiolytic activity of Nymphaea alba Linn. in mice as experimental models of anxiety. Indian J Pharmacol. 2011; 43(1): 50-55.d
14. S. Gomathi, R. Shanmuga Sundaram, M. Vijayabaskaran, C. Kannan, R. Sambathkumar. Pedalium murex Linn leaves against LPS-induced oxidative stress, anxiety and depression behavioural alterations in rats. Research J. Pharm. and Tech. 2017; 10(5): 1333-1338.
15. Roopesh Poojary, Nayanatara Arun Kumar, Reshma Kumarchandra2, Ganesh Sanjeev. Cynodon dactylon alleviates radiation-induced behavioral and biochemical changes in the cerebral cortex of mice. Research Journal of Pharmacy and Technology. 2021; 14(5):
16. Farrahdilla Hamzah, Khairi Che Mat, Vidya Bhagat, Safiya Amaran, Haszalina Hassan. Assessing Test Anxiety among the First Year Nursing Students’ of University Sultan Zainal Abidin. Research J. Pharm. and Tech. 2018; 11(4): 1448-1451.
17. Bharat Mishra, Elezabeth John. A Systematic Review on Neuro-Psychopharmacological effects of Celastrus paniculatus (Malkangani) Oil. Research J. Pharm. and Tech. 2020; 13(5):2452-2458.
18. Imad Hadi Hameed, Maria Rosario Calixto Cotos, Mohammed Yahya Hadi. Antimicrobial, Antioxidant, Hemolytic, Anti-anxiety, and Antihypertensive activity of Passiflora species. Research J. Pharm. and Tech 2017; 10(11): 4079-4084.
19. Rupa Joshi, K. H. Reeta, Surinder Kumar Sharma, Manjari Tripathi, Yogendra Kumar Gupta. Pharmacodynamics and pharmacokinetic interaction of panchagavya ghrita with phenytoin and carbamazepine in maximal electrical shock induced seizures in rat. AYU 2015 April- June,36(2):196-202.
20. Role of panachagavya ghrita in the management of Atattvabhinivesha (Obsessive Compulsive Disorder). IAMJ 2015 September,3(9):2637-2646.
21. Bharathirajan M, L. Jeyanthi Rebecca, M. Jothi Muniyandi. Study on the Antibacterial Activity and Identification of cellulolytic bacteria from Cow Urine. Research J. Pharm. and Tech 2018; 11(9): 3993-3997.