Author(s): Rollando Rollando, Warsito Warsito, Masruri Masruri, Nashi Widodo

Email(s): ro.llando@machung.ac.id

DOI: 10.52711/0974-360X.2022.00884   

Address: Rollando Rollando1,2*, Warsito Warsito2, Masruri Masruri2, Nashi Widodo3
1Pharmacy Department, Faculty of Science and Technology, Ma Chung University, Malang 65151, Indonesia.
2Doctoral Student, Chemistry Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, Indonesia.
3Chemistry Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, Indonesia.
4Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 11,     Year - 2022


ABSTRACT:
In our previous study, aurone compound were found in the n-butanol fraction of Sterculia quadrifida leaves. The aurone compound (compound 1) had high cytotoxic activity in cell lines with IC50 values of 4.05, 12.53, 15.38, and 25.91 µg/mL in 4T1, MCF7, MDA-MB-435, T47D, respectively. In this study, we performed the MMP-9 enzyme inhibition test with FRET assay, and to deepen the mechanism of action of the compounds use molecular docking and molecular dynamics. The results showed that compound 1 had the MMP-9 enzyme inhibition of 91.23% and IC50 48.27%. Furthermore, molecular docking and molecular dynamic simulation suggest compound 1 interacts firmly and stably at the enzyme catalytic site. In conclusion, this study can provide important information on the development of herbal medicines that are potentially cancer drugs.


Cite this article:
Rollando Rollando, Warsito Warsito, Masruri Masruri, Nashi Widodo. Potential matrix metalloproteinase-9 inhibitor of aurone compound isolated from Sterculia quadrifida leaves: In-vitro and in-silico studies. Research Journal of Pharmacy and Technology. 2022; 15(11):5250-4. doi: 10.52711/0974-360X.2022.00884

Cite(Electronic):
Rollando Rollando, Warsito Warsito, Masruri Masruri, Nashi Widodo. Potential matrix metalloproteinase-9 inhibitor of aurone compound isolated from Sterculia quadrifida leaves: In-vitro and in-silico studies. Research Journal of Pharmacy and Technology. 2022; 15(11):5250-4. doi: 10.52711/0974-360X.2022.00884   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-11-69


REFERENCES:
1.    Sung H. Ferlay J. Siegel R.L. Laversanne M. Soerjomataram I. Jemal A. Bray F. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: a cancer journal for clinicians, 2021; 71(3): 209–249.doi.org/10.3322/caac.21660
2.    Latest global cancer data: Cancer burden rises to 19.3 million new cases and 10.0 million cancer deaths in 2020 QUESTIONS AND ANSWERS (Q&A) – IARC. (n.d.). Retrieved November 27, 2021, from https://www.iarc.who.int/faq/latest-global-cancer-data-2020-qa/
3.    Figueroa J.D. Gray E. Pashayan N. Deandrea S. Karch A.Vale D.B. Nickson C. The impact of the Covid-19 pandemic on breast cancer early detection and screening. Preventive Medicine. 2021; 151(1), 10-16. doi.org/10.1016/j.ypmed.2021.106585
4.    Zhang J. Wei Q. Dong D. Ren L. The role of TPS, CA125, CA15-3 and CEA in prediction of distant metastasis of breast cancer. Clinica Chimica Acta. 2021; 523(3), 19–25. doi.org/10.1016/j.cca.2021.08.027
5.    Yin L. Duan J.J. Bian X.W. Yu S.C. Triple-negative breast cancer molecular subtyping and treatment progress. Breast Cancer Research: BCR. 2020; 22(1): 61-78. doi.org/10.1186/s13058-020-01296-5
6.    Gautam J. Banskota S. Lee H. Lee Y.J. Jeon Y.H. Kim J.A. Jeong B.S. Down-regulation of cathepsin S and matrix metalloproteinase-9 via Src, a non-receptor tyrosine kinase, suppresses triple-negative breast cancer growth and metastasis. Experimental & Molecular Medicine. 2018; 50(9):1–14.doi.org/10.1038/s12276-018-0135-9
7.    Hariono M. Rollando R. Karamoy J. Hariyono P. Atmono M. Djohan M. Wahab H. Bioguided fractionation of local plants against matrix metalloproteinase9 and its cytotoxicity against breast cancer cell models: in silico and in vitro study. Molecules.2020; 25(20). doi.org/10.3390/molecules25204691
8.    Rollando R. Warsito W. Masruri M. Widodo W. Sterculia foetida leaf fraction against matrix metalloproteinase-9 protein and 4t1 breast cancer cells: in-vitro and in-silico studies. Tropical Journal of Natural Product Research. 2021; 5(1): 113–121.doi.org/10.26538/tjnpr/v5i1.15
9.    Otuokere I.E. Amaku F.J. Alisa C.O. In silico geometry optimization, excited – state properties of (2e)-n-hydroxy-3-[3- (phenylsulfamoyl) phenyl] prop-2-enamide (belinostat) and its molecular docking studies with ebola virus glycoprotein. Asian Journal of Pharmaceutical Research. 2015; 5(3): 131-137. doi. 10.5958/2231-5691.2015.00020.9
10.    Rutuja P. P. Sachin H. R. Role of autodock vina in pyrx molecular docking. Asian Journal of Research in Chemistry. 2021; 14(2):132-134. doi: 10.5958/0974-4150.2021.00024.9
11.    Hariono M. Nuwarda R.F. Yusuf M. Rollando R. Jenie R.I. Al-Najjar, Wahab H.A. Arylamide as potential selective inhibitor for matrix metalloproteinase 9 (mmp9): design, synthesis, biological evaluation, and molecular modeling. Journal of Chemical Information and Modeling. 2020; 60(1): 349–359.doi.org/10.1021/acs.jcim.9b00630
12.    Xavier S.M Mahalakshmi J.A. Vinisha G. Computational modeling of novel drug targets ftse and mpt83 for mdrtb and molecular docking of selected compounds from medicinal plants. Research Journal of Pharmacy and Technology. 2018; 11(4): 1413-1420. doi.10.5958/0974-360X.2018.00264.0
13.    Vijayakumar V. Radhakrishnan N.  Vasantha-Srinivasan P. Molecular docking analysis of triazole analogues as inhibitors of human neutrophil elastase (hne), matrix metalloproteinase (mmp 2 and mmp 9) and tyrosinase. Research Journal of Pharmacy and Technology. 2020; 13(6): 2777-2783. doi.10.5958/0974-360X.2020.00493.X
14.    Skariyachan S. Gopal D. Chakrabarti S. Kempanna P. Uttarkar A. Muddebihalkar A.G. Niranjan V. Structural and molecular basis of the interaction mechanism of selected drugs towards multiple targets of SARS-CoV-2 by molecular docking and dynamic simulation studies- deciphering the scope of repurposed drugs. Computers in Biology and Medicine. 2020; 126(4):10-14. doi.org/10.1016/j.compbiomed.2020.104054
15.    Dong H. Diao H. Zhao Y. Xu H. Pei S. Gao J. Lin D. Overexpression of matrix metalloproteinase-9 in breast cancer cell lines remarkably increases the cell malignancy largely via activation of transforming growth factor beta/SMAD signalling. Cell Proliferation. 2019; 52(5):25-34.doi.org/10.1111/cpr.12633
16.    Huang H. Matrix metalloproteinase-9 (mmp-9) as a cancer biomarker and mmp-9 biosensors: recent advances. Sensors. 2018;18(10):67-74.doi.org/10.3390/s18103249
17.    Aloss K. Jdeed S. Alshehabi. J. Detecting the role of CCR7-CCL21/CCL19 axis in breast cancer progression and lymph node metastasis incidence. Research Journal of Pharmacy and Technology. 2018; 11(1): 231-235. doi.10.5958/0974-360X.2018.00043.4
18.    Mohammadian H. Sharifi R. Rezanezhad A.S. Taheri E. Babazadeh B.A. Matrix metalloproteinase MMP1 and MMP9 genes expression in breast cancer tissue. Gene Reports. 2020; 21(5): 10-19.doi.org/10.1016/j.genrep.2020.100906
19.    Jainab N.H. Raja M.K.M.M. In silico molecular docking studies on the chemical constituents of clerodendrum phlomidis for its cytotoxic potential against breast cancer markers. Research Journal of Pharmacy and Technology. 2018; 11(4): 1612-1618. doi.10.5958/0974-360X.2018.00300.1
20.    Anggi V. Adikusuma W. Total antioxidant and in-vitro cytotoxic of Abelmoschus manihot (L.) medik from palu of central sulawesi and doxorubicin on 4T1 cells line and Vero cells. Research Journal of Pharmacy and Technology. 2019; 12(11): 5472-5476. doi.10.5958/0974-360X.2019.00949.1  
21.    Novitasari D. Jenie R.I. Wulandari F. Utomo R.Y. Putri D.D.P. Kato J. Meiyanto E. Curcumin-like structure (CCA-1.1) induces permanent mitotic arrest (Senescence) on Triple-negative breast cancer (TNBC) cells, 4T1. Research Journal of Pharmacy and Technology. 2021; 14(8):4375-2. doi.10.52711/0974-360X.2021.00760
22.    Wu H.T. Lin J. Liu Y.E. Chen H.F. Hsu K.W. Lin S.H. Chen D.R. Luteolin suppresses androgen receptor-positive triple-negative breast cancer cell proliferation and metastasis by epigenetic regulation of MMP9 expression via the AKT/mTOR signaling pathway. Phytomedicine.2021; 81(3): 15-35.doi.org/10.1016/j.phymed.2020.153437
23.    Li L. Fan P. Chou H. Li J. Wang K. Li H. Herbacetin suppressed MMP9 mediated angiogenesis of malignant melanoma through blocking EGFR-ERK/AKT signaling pathway. Biochimie. 2019; 162(6):198–207.doi.org/10.1016/j.biochi.2019.05.003
24.    Si L. Fu J. Liu W. Hayashi T. Nie Y. Mizuno K. Ikejima T. Silibinin inhibits migration and invasion of breast cancer MDA-MB-231 cells through induction of mitochondrial fusion. Molecular and Cellular Biochemistry. 2020; 463(2):189–201.doi.org/10.1007/s11010-019-03640-6
25.    Li Y. Gan C. Zhang Y. Yu Y. Fan C. Deng Y. Yin W. Inhibition of stat3 signaling pathway by natural product pectolinarigenin attenuates breast cancer metastasis. Frontiers in Pharmacology. 2019; 10(2): 89-95.doi.org/10.3389/fphar.2019.01195
26.    Firoj A.T. Harinath N. More inhibitory effects of successive solvant extracts of Barleria gibsoni Dalz. on the proliferation of MDA MB 4355 (human breast cancer) and Hep G2 (liver cancer cell line). Asian Journal of Pharmaceutical Research. 5(4): 2015; 183-185. doi.10.5958/2231-5691.2015.00028.3
27.    Lavanya M. Bhaumik A. Reddy A.G. Manasa H. Kalyani B. Sushmitha.S. Evaluation of anticancer activity of ethanolic and ethylacetoacetate extracts of sweet cherry against human breast cancer cell line MCF-7. Research Journal of Pharmacology and Pharmacodynamics. 2016; 8(2): 65-70. doi. 10.5958/2321-5836.2016.00012.4

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