Vinay NS, Pooja T, Nandeesh R, Syed Mansoor Ahmed, Veeresh P Veerapur, Babitha S
Vinay NS1, Pooja T1, Nandeesh R2, Syed Mansoor Ahmed1, Veeresh P Veerapur3, Babitha S1*
1Department of Pharmacology, Sree Siddaganga College of Pharmacy, Tumakuru, India.
2 Department of Pharmacognosy, Sree Siddaganga College of Pharmacy, Tumakuru, India.
3Department of Quality Assurance, Sree Siddaganga College of Pharmacy, Tumakuru, India.
Volume - 15,
Issue - 11,
Year - 2022
The anti-inflammatory potential of methanol and ethyl acetate fractions of Sesbania grandiflora Linn., roots were evaluated by carrageenan induced rat paw oedema method and cotton pellet induced granuloma in rats. Female Wistar rats were treated orally with methanol (MFSGR) and ethyl acetate (EAFSGR) fractions of Sesbania grandiflora roots (100 and 200 mg/kg) and Diclofenac sodium (15 mg/kg). One-hour post treatment, Carrageenan (0.1 ml of 1 % w/v, s.c.) was administered in the sub plantar region of the left hind limb of rat. Thereafter, paw volume was measured at 0, 30 min, 1, 2, 3 and 4 h. Percentage inhibition (PI) of oedema was calculated. In cotton pellet-induced granuloma model, male Wistar rats were treated with Diclofenac sodium (10 mg/kg, p.o.) and MFSGR and EAFSGR (100 and 200 mg/kg, p.o.) respectively. Thirty-minutes after respective treatment, an autoclaved cotton pellet of 20±1 mg was aseptically implanted subcutaneously. Fraction and standard diclofenac were administered once daily for the next 7 days. On 8th day, excised cotton granuloma was dried and weighed, body weight and organ index were calculated. MFSGR and EAFSGR (100 and 200 mg/kg) exhibited a significant inhibition in paw volume against carrageenan-induced paw oedema. In cotton pellet induced granuloma model, MFSGR and EAFSGR exhibited significant inhibition of cotton dry and wet weight. The present study suggests that the methanol and ethyl acetate fractions of Sesbania grandiflora roots exhibit anti-inflammatory activity. The observed effect could be attributed to the presence of vital phytoconstituents.
Cite this article:
Vinay NS, Pooja T, Nandeesh R, Syed Mansoor Ahmed, Veeresh P Veerapur, Babitha S. Anti-inflammatory activity of methanol and ethyl acetate fractions of Sesbania grandiflora roots in rats. Research Journal of Pharmacy and Technology. 2022; 15(11):5115-9. doi: 10.52711/0974-360X.2022.00860
Vinay NS, Pooja T, Nandeesh R, Syed Mansoor Ahmed, Veeresh P Veerapur, Babitha S. Anti-inflammatory activity of methanol and ethyl acetate fractions of Sesbania grandiflora roots in rats. Research Journal of Pharmacy and Technology. 2022; 15(11):5115-9. doi: 10.52711/0974-360X.2022.00860 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-11-45
1. Killedar SG and HN More. Analgesic and anti-inflammatory studies of Memecylon umbellatum Burm roots in experimental animals. Research J. Pharm. and Tech. 2009; 2 (4):858-861.
2. Karim N, Khan I, Khan W, et al. Anti-nociceptive and anti-inflammatory activities of Asparacosin A involve selective cyclooxygenase 2 and inflammatory cytokines inhibition: An in-vitro, in-vivo, and in-silico approach. Frontiers in immunology. 2019; 10:581. doi: 10.3389/fimmu.2019.00581.
3. Patel T, Dudhpejiya A, Sheath N. Anti inflammatory activity of Myrica nagi Linn. Bark. Ancient science of life. 2011; 30(4):100-103. PMCID: PMC3336264
4. Cai C, Chen Y, Zhong S, et al. Anti-inflammatory activity of N-butanol extract from Ipomoea stoloniferain vivo andin vitro. PLoS One. 2014; 9(4):e95931. doi:10.1371/journal.pone.0095931.
5. Hasan N, Osman H, Mohamad S, et al. The chemical components of Sesbania grandiflora root and their antituberculosis activity. Pharmaceuticals. 2012;5(8):882-889. doi:10.3390/ph5080882.
6. Sreelatha S, Padma PR, Umasankari E. Evaluation of anticancer activity of ethanol extract of Sesbania grandiflora (Agati Sesban) against Ehrlich as cites carcinoma in Swiss albino mice. Journal of ethno pharmacology. 2011;134(3):984-987. doi:10.1016/j.jep.2011.01.012.
7. Ramesh T, Sureka C, Bhuvana S, et al. Sesbania grandiflora diminishes oxidative stress and ameliorates antioxidant capacity in liver and kidney of rats exposed to cigarette smoke. Journal of Physiology and Pharmacology. 2010; 61(4):467-476. PMID:20814075.
8. Pari L, Uma A. Protective effect of Sesbania grandiflora against erythromycin estolate-induced hepatotoxicity. Therapies. 2003; 58(5):439-443.
9. Karthikeyan P, Suresh V, Suresh A et al. Wound healing activity of Sesbania grandiflora (L.) poir. Bark. Int J of Pharm. Research & Development. 2011; 3(2):87-93.
10. Bhalke RD, Giri MA, Anarthe SJ, Pal SC. Antiulcer activity of the ethanol extract of leaves of Sesbania grandiflora (Linn.). International Journal of Pharmacy and Pharmaceutical Sciences. 2010;2(4):206-208.
11. Ouattara MB, Konate K, Kiendrebeogo M et al. Antibacterial potential and antioxidant activity of polyphenols of Sesbania grandiflora. Curr Res J of Biol Sci. 2011;3(4):351-356.
12. Kurian JC. Plants that heal. Pune: Oriental watchman publication house; 2004.
13. Venkateshwarlu Goli, Kanakam Vijay Bhaskar et al. Effects of anti-inflammatory activity of Mimosa pudica. Asian J. Pharm. Res. 2011; 1(3): 69-71.
14. SheshadriShekar D, C Velmurugan, G Ramakrishnan and B Vivek. Anti-inflammatory activity of ethanolic and acetone extracts of Bauhnia Variegate. Research J. Pharm. and Tech. 2009; 2(2):409-411.
15. Sambrekar Sudhir N, Patil PA and Suhas A Patil. Anti-inflammatory effect of the Embelia tsjeriam -Cottam fruit extracts. Research J. Pharmacology and Pharmacodynamics. 2011; 3(5): 246-249.
16. Shraddha K More, Anirudha A Lande, Priti A Jagadale et al. Evaluation of anti-inflammatory activity of Solanum xanthocarpum Schrad and Wendl (Kaṇṭakari) extract in laboratory animals. Ancient science of life. 2013; 32(4):222-226. DOI: 10.4103/0257-7941.131976.
17. Vogel HG, editor. Drug discovery and evaluation: pharmacological assays. Springer Science & Business Media; 2002.
18. Panthong A, Kanjanapothi D, Taesotikul T et al. Anti-inflammatory activity of methanolic extracts from Ventilago harmandiana Pierre. Journal of Ethnopharmacology. 2004; 91(2-3):237-242. doi: 10.1016/j.jep.2003.12.025.
19. Gonzalez R, Ballester I, Lopez-Posadas R et al. Effects of flavonoids and other polyphenols on inflammation. Critical reviews in food science and nutrition. 2011;51(4):331-362. DOI: 10.1080/10408390903584094.
20. Mira L, Tereza Fernandez M, Santos M et al. Interactions of flavonoids with iron and copper ions: a mechanism for their antioxidant activity. Free radical research. 2002; 36(11):1199-208. DOI: 10.1080/1071576021000016463.
21. Srisawat U, Panunto W, Kaendee N et al. Determination of phenolic compounds, flavonoids, and antioxidant activities in water extracts of Thai red and white rice cultivars. J Med Assoc Thai. 2011;94(7):896. DOI: 10.1055/s-0030-1264431.
22. Saxena M, Saxena J, Pradhan A. Flavonoids and phenolic acids as antioxidants in plants and human health. Int. J. Pharm. Sci. Rev. Res. 2012; 16(2):130-134.