Author(s): X. Asbin Mary, M. Syed Ali, V. Anuradha, N. Yogananth, J. Vardhana, P. Vinoth Kumar

Email(s): syedmicro555@gmail.com

DOI: 10.52711/0974-360X.2022.00844   

Address: X. Asbin Mary1,3, M. Syed Ali1*, V. Anuradha2, N. Yogananth1, J. Vardhana3, P. Vinoth Kumar4
1Post Graduate and Research Department of Biotechnology, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai - 600119, Tamil Nadu, India.
2Post Graduate and Research Department of Biochemistry , Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai - 600119, Tamil Nadu, India.
3Department of Biotechnology, Vels Institute of Science Technology and Advanced Studies, Chennai-600043, Tamilnadu, India.
4Department of Microbiology, Nehru Arts and Science College, Coimbatore - 641105, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 11,     Year - 2022


ABSTRACT:
The tropical red mangrove Rhizophora mucronata (Lam.) has been widely used as an astringent, antiseptic, antibacterial, anti-ulcerogenic, and anti-inflammatory agent in traditional Oriental medicine. Cancer is a concept to be characterized by differentiating features, uncontrolled proliferation of cells, invasion of abnormal cells into healthy cells, and their spread through the bloodstream to vital organs. The key programmed cell death mechanism for silently killing unwanted and detrimental cells during embryonic development, tissue homeostasis, and immune regulation is apoptosis. Our research investigated the activation of apoptosis by the methanolic extract of R. mucronata and the expression of apoptotic and anti-apoptotic proteins. The cell cycle and apoptosis were observed by the Annexin V/PI-flow cytometry technique. The MTT test showed a strong cytotoxic effect on the cell lines of MCF-7. In MCF-7 cells, the sample demonstrated successful cell cycle arrest. More number of MCF7 cells get arrested in Sub G0/G1. In our study, FITC Rabbit Anti-Active Caspase 3 is used as a fluorochrome. It can be inferred from our study that good therapeutic potential against Human Breast derived diseases can be compared to the std control and the compound samples.


Cite this article:
X. Asbin Mary, M. Syed Ali, V. Anuradha, N. Yogananth, J. Vardhana, P. Vinoth Kumar. Induction of Apoptosis by Methanolic Extract of Rhizophora mucronata Lam on MCF-7 Cell Line. Research Journal of Pharmacy and Technology. 2022; 15(11):5021-5. doi: 10.52711/0974-360X.2022.00844

Cite(Electronic):
X. Asbin Mary, M. Syed Ali, V. Anuradha, N. Yogananth, J. Vardhana, P. Vinoth Kumar. Induction of Apoptosis by Methanolic Extract of Rhizophora mucronata Lam on MCF-7 Cell Line. Research Journal of Pharmacy and Technology. 2022; 15(11):5021-5. doi: 10.52711/0974-360X.2022.00844   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-11-29


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