Author(s): Vaibhav. S. Patil, Varashree B S, K. Sreedhara Ranganath Pai, Gangadhar Hari, Keerthi Priya

Email(s): varashree.bs@manipal.edu

DOI: 10.52711/0974-360X.2022.00833   

Address: Vaibhav. S. Patil1, Varashree B S2*, K. Sreedhara Ranganath Pai3, Gangadhar Hari4, Keerthi Priya5
1Department of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher education (MAHE), Manipal 576104, Karnataka, India.
2Professor and Head, Department of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher education (MAHE), Manipal 576104, Karnataka, India.
3Professor and Head, Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education (MAHE), Manipal-576104, Karnataka, India.
4Research Scholar, Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education (MAHE), Manipal-576104, Karnataka, India.
5Research Scholar, Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education (MAHE), Manipal-576104, Karnataka, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 11,     Year - 2022


ABSTRACT:
Cytotoxic effects of chloroform-methanol extract, protein extract and various fractions of ethanolic extracts of Curcuma amada rhizome were investigated on Human colorectal cancer cell line (HCT116). The ethanolic extract showed excellent cytotoxicity of all extracts with an IC50 value of 14.46µg followed by the Chloroform-methanol extract with an IC50 value of 18.31µg. The various fractions of ethanolic extracts fractionated with petroleum ether, ethyl acetate, n-butanol were also tested for cytotoxicity independently. Ethyl acetate fraction gave an IC50 value of 1012µg and n-butanol IC50 value was 43.19µg, with petroleum ether fraction giving no cytotoxicity. The crude proteins isolated from the rhizome showed good cytotoxicity and gave an IC50 of 43.1µg.


Cite this article:
Vaibhav. S. Patil, Varashree B S, K. Sreedhara Ranganath Pai, Gangadhar Hari, Keerthi Priya. Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell linew. Research Journal of Pharmacy and Technology. 2022; 15(11):4955-8. doi: 10.52711/0974-360X.2022.00833

Cite(Electronic):
Vaibhav. S. Patil, Varashree B S, K. Sreedhara Ranganath Pai, Gangadhar Hari, Keerthi Priya. Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell linew. Research Journal of Pharmacy and Technology. 2022; 15(11):4955-8. doi: 10.52711/0974-360X.2022.00833   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-11-18


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